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Biomedical subjects

G Georgiev

Publications and source records attributed to G Georgiev.

At least 145 records · Page 8Linked to original sources
PubMedDate not supplied

Tissue-specific posttranscriptional downregulation of expression of the S100A4(mts1) gene in transgenic animals.

The S100A4(mts1) is a gene associated with generation of metastatic disease. In order to analyze the consequences of alteration of the pattern of expression of the S100A4(mts1) gene we obtained strains of transgenic mice bearing the S100A4(mts1) gene under the control of a ubiquitous and constitutive 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR) gene promoter. In transgenic animals the expression of the transgene RNA was detected in all organs, but only some of the organs showed elevated levels of the protein. Expression of the S100A4(Mts1) protein was downregulated in the organs that normally do not express the gene in the wild-type animal. The transgene RNA is detected in the polysomes indicating that it could be translated into the S100A4(Mts1) protein. The specificity of the S100A4(Mts1) protein expression is determined by a complex mechanism including regulation of translation and/or posttranslational degradation.

Animals↗
PubMedDate not supplied

[Effect of prolonged hyperoxia on rabbit lung lipids and their fatty acid content (author's transl)].

The effect of a 36-hour hyperoxia (60 % oxygen) on rabbit lung lipids has been investigated. It has been established that hyperoxia leads to the increase of all lipid fractions in lung tissue and alveolar surfactant, phospholipid and cholesterol being most significantly affected. It has also been found that hyperoxia leads to the decrease of palmitic acid and to the increase of linoleic and arachidonic acids almost in all lipid fractions. Marked changes in the positional distribution of fatty acids in alveolar surfactant phosphatidylcholine have also been observed. The possible mechanisms of the effect of oxygen on lung lipid metabolism have been discussed, and the consequences ensuing from it which could affect the structural organization of membranes have been evaluated.

Animals↗
PubMedDate not supplied

In vitro effect of insulin and adrenaline on lung triglyceride-lipase activity in rats.

The in vitro effect of insulin and adrenaline on the activity of lung triglyceride-lipases (alkaline and acid) has been investigated. Insulin inhibited strongly both triglyceride-lipases. Only caffein almost eliminated the inhibitory action of insulin, while adrenaline and dibutyryl cyclic adenosine monophosphate did not exhibit such an effect. It was assumed that the inhibition of lung triglyceride-lipases by insulin was effected through the activation of phosphodiesterases. On the other hand since adrenaline markedly activated lung triglyceride-lipases, this action was assumed to be carried out via the activation of lung adenylate cyclase and the increase of cyclic adenosine monophosphate.

Adenylyl Cyclases↗
PubMedDate not supplied

[The Pulma-01 automated microprocessor system for testing external respiratory function].

The automated system consisting of a pneumotachographic transducer, a differential pressure gauge, a precision amplifier and a microcomputer with a built-in printer is designed for the analysis of 20 main indices of external respiration functioning during forced expiration. It evaluates the measured data, compares them with the normal values and automatically diagnoses the pulmonary disease type.

Computers↗
PubMedDate not supplied

Insulin effect on lung subcellular fraction phospholipids and cholesterol.

Lung subcellular fraction (microsomes, mitochondria, 105 000 X g supernatant and plasma membranes) were isolated from normal and insulin treated rats. Phospholipid and cholesterol contents were determined, phosphatidylcholines and phosphatidyl-ethanolamines separated and their fatty acid profiles assayed. Teh investigations carried out indicated that insulin influenced the phospholipid content of all fractions. Cholesterol levels in the 105 000 X g supernatant and especially in the plasma membrane fraction were raised significantly. Insulin administration increased the contribution of the unsaturated long chain fatty acids in both phospholipids studied. The results are discussed in relation to stimulated lipid biosynthesis and to lipid fluidity, of which fatty acyl unsaturation and cholesterol level are main parameters.

Animals↗