Biomedical subjects

G G Polianskaia

Publications and source records attributed to G G Polianskaia.

At least 19 recordsLinked to original sources

PubMed2005

[Continuous human embryonic stem cell lines].

A new continuous human embryonic stem cell line (HESC-5) derived from a blastocyst is described. The cultured cell passed over 200 population doublings, which exceeds the Hayflick's limit sufficiently. The cells maintained a stable proliferative activity, high activity of alkaline phosphatase, and expression of transcription factor Oct-4 and of surface antigens SSEA-3, SSEA-4 and TRA-1-60 known to be characteristic of embryonic stem cells of the human origin. Immunofluorescent detection of antigens, characteristic of ectoderm, endoderm and mesoderm in the new cell line HESC-5, and in the previously described other four stem cell lines confirms the ability of these cells to retain their pluripotency under in vitro condition. In addition, in all the cell lines, a high telomerase activity was revealed, which controls a stable telomere length and, hence, an unlimited ESC proliferation. Unlike other cell lines, HESC-5 was found, under specific conditions, to spontaneously differentiate into hematopoietic cells. A morphological similarity was shown between ESC colonies cultivated both on a feeder layer and in the non-feeder system.

Blastocyst↗

PubMed2005

[The effect of immobilized fibronectin on karyotypic variability in the skin fibroblast cell subline of indian muntjac].

The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblast cell subline MT on cultivating cells on the fibronectin-coated surface. In cell subline MT, cultivated on the fibronectin-coated surface for 1 and 2 days, the character of cell distribution for the chromosome number did not change. In 3, 4 and 8 days, the character of cell distribution for the chromosome number changed. These changes involve a significant decrease in frequency of cells with modal numbers of chromosomes, and an increase in frequency of cells with lower chromosome numbers. Many new additional structural variants of the karyotype (SVK) appear. The observed alterations seem to be due to both disturbances of mitotic apparatus and selection of SVK, which are more advantageous to changed culture conditions of the cell population. Detachment of cells from the fibronectin-coated surface, followed by a 1 day cultivation on a hydrophilic surface, commonly used for routine cell cultivation, does not restore the control cell distribution for the chromosome number, but cultivation in these conditions for 5 days restore control distribution. The frequency of chromosomal aberrations on cultivation on the fibronectin-coated surface for 3 and 4 days significantly increases, mainly at the expence of dicentrics (telomeric association). On prolongating the time of cultivation up to 8 days on the fibronectin-coated surface the frequency of chromosomal aberrations approaches the control value. Structural instability of chromosomes at cultivation on the fibronectin-coated surface demonstrates nonspecific reaction of "markerless" cell lines to unfavourable factors of the environment. We discuss possible reasons of differences in the character of karyotypic variability in cell lines of the Indian muntjac skin fibroblasts on cultivating on laminin and fibronectin.

Animals↗

PubMed2003

[Effect of laminin on numerical karyotype variability of kangaroo rat kidney cell lines].

The numerical karyotypic variability has been investigated in "markerless" epithelial-like Rat kangaroo kidney cell lines NBL-3-11 and NBL-3-17 on cultivation on a laminin-2/4 coated surface. In cell line NBL-3-17, cultivated on the laminin-coated surface for 2, 4 and 12 days, the character of numerical karyotypic variability has changed. In 2 days the general character of cell distribution for the chromosome number did not change, but the frequency of cells with modal number of chromosomes decreases significantly, while that of cells with lower chromosome number show a tendency to increase. At a prolongation of cultivation time to 4 and 12 days, the numerical karyotypic heterogeneity in cell population increases due to a significant change in the general character of cell distribution for the chromosome number, which is caused by a significant decrease in the frequency of cells with the modal number of chromosomes, and by an increase in the frequency of cells with lower chromosome number. The analysis of distribution of individual chromosomes showed that the number of types of additional structural variants of the karyotype (SVK) increases significantly on cultivation on laminin for 2-12 days. In cell line NBL-3-11, cultivated on the laminin-coated surface for 2 and 4 days, the character of numerical karyotypic variability did not change compared to control variants. Possible reasons of the observed changes of numerical karyotypic variability in cell line NBL-3-17 is discussed. The reason of differences in the character of numerical karyotypic variability between cell lines NBL-3-11 and NBL-3-17 possibly consists in the change of gene expression, namely in a dose of certain functioning genes. The polymerase chain reaction with arbitrary primers revealed no differences between DNA patterns of cell lines NBL-3-17 and NBL-3-11. This can reflect a similarity in the primary DNA structure of both cell lines. Hence, these lines differ only in the number of homologous chromosomes (hypotriploid and hypodiploid).

Animals↗

PubMed2003

[Effect of laminin on structural karyotype variability of kangaroo rat kidney cell lines].

The structural karyotypic variability has been investigated in the "markerless" epithelial-like Rat kangaroo kidney cell lines NBL-3-17 and NBL-3-11 on cultivation on a laminin-2/4 coated surface. In cell line NBL-3-17, cultivated on the laminin-coated surface for 2, 4 and 12 days, and in cell line NBL-3-11, cultivated on the laminin-coated surface for 2 and 4 days, there is a significant increase in the frequency of chromosomal aberrations, both chromosomal breaks and dicentrics (telomeric associations). Different sensitivity of individual chromosomes to inducing chromosomal breaks was observed in addition to a preferential involvement of some chromosomes in dicentric formation. Structural instability of chromosomes at cultivation on laminin demonstrates nonspecific reaction of the "markerless" cell lines to unfavourable factors of the environment. We discuss possible reasons of differences in the character of karyotypic variability between a cell line of the Indian muntjac skin fibroblasts and epithelial-like Rat kangaroo kidney cell lines cultivated on laminin.

Animals↗

PubMed2003

[Isolation and characterisation of continuous human embryonic stem cell lines].

A long-term cultivation (5-8 months) of human blastocyst-derived embryonic cells (hES) was performed. Several properties of hESs were examined to prove the state of continuous cell lines. These cells have passed through 100-175 population doublings with the average population doubling time equal to 37.0 +/- 1.5 h. Isolated hESs, referred to as HESC-1, HESC-2, HESC-3, HESC-4, cultivated on mitotically inactivated mouse embryonic fibroblasts (STO continuous cell line), formed multilayer colonies of various shape. The cells maintained stable proliferative activity, high activity of alkaline phosphatase and expression of transcription factor Oct4, and all this characterizes embryonic stem cells of different origin. Expression of hES specific cell surface antigens (SSEA-3, SSEA-4, TRA-1-81 and TRA-11-81 and TRA-1-60) was confirmed by immunofluorescence analysis with the corresponding monoclonal antibodies. An additional prove for species specificity of HESC lines is the lack of expression of mouse specific surface antigen SSEA1. The cell cycle of HESC-1 undifferentiated cells and embryoid bodies was analysed cytofluorimetrically.

Alkaline Phosphatase↗

PubMed2002

[Effect of laminin on karyotypic variability in cell line of Indian muntjac skin fibroblasts].

The numerical and structural karyotypic variability has been investigated in the Indian muntjac skin fibroblasts cell line M and karyotypic variant of this line M' on cultivation on a laminin 2/4 coated surface. In cell line M, cultivated on the laminin-coated surface for 4 and 14 days, and in karyotypic variant M', cultivated for 2, 4 and 14 days, the character of cell distribution for the chromosome number has changed. These changes involve a significant decrease in frequency of cells with model numbers of chromosomes, and an increase in frequency of cells with lower chromosome numbers. As a result, new modal chromosome numbers form. The frequency of cells with 4 chromosomes increases significantly; as a rule, such cells are absent in the control cell variants. Many new additional structural variants of the karyotype (SVK) appear. Detachment of cells M' from the laminin-coated surface followed by a 2 day cultivation on a hydrophilic surface, commonly used for routine cell cultivation, does not restore the control cell distribution for chromosomal number. The frequency of chromosomal aberrations on cultivation of the laminin-coated surface does not change relatively to controls. The observed alterations seem to be due to both disturbances of mitotic apparatus and selection of SVK, which are more advantageous to changed culture conditions of the cell population.

Animals↗

PubMed2000

[The effect of mycoplasmal contamination of human embryonic lung cell line MRC-5 on the karyotypic variability].

Karyotypic variability has been investigated for nonimmortalized human embryonic lung cell line MRC-5, cultivated with Acholeplasma laidlawii strain PG-8 for 15-45 days. The character of cell distribution for chromosome number did not change during this time. In all investigated variants the number of polyploid cells increased considerably with the lengthening of the term after decryoconservation. The number of chromosomal aberrations in 15-45 days contaminated cells increased significantly as compared to the control at the expense of dicentrics (telomeric associations). The number of dicentrics had a tendency to increase with the lengthening of the term of contamination. Thus, in 45 days the number of dicentrics increased twice as much as that in 15 days. The increase of polyploids may be due presumably to the specific character of karyotypic variability in nonimmortalized cell lines with the long-term cultivation. Our present and previous results made it possible to suppose that the formation of dicentrics (telomeric associations) in nonimmortalized "markerless" cell line, following the long-term mycoplasmal contamination, may prove additionally the role played by dicentrics in cell adaptation to in vitro conditions whatever the degree of transformation may be--nonimmortalized line or immortalized nontumorogenic or high tumorogenic lines.

Acholeplasma laidlawii↗

PubMed2000

[Effect of mycoplasma contamination of a human cervical carcinoma cell line M HeLa clone 11 on karyotypic variability].

The karyotypic variability has been investigated for an immortalized human epithelioid cervix carcinoma cell line M HeLa clone 11, cultivated for 15-60 days after contamination with Acholeplasma laidlawii A, strain PG-8, and for 30 days after contamination with Mycoplasma arginini R-16. The character of cell distribution for chromosome number changes in contaminated cells significantly, as compared to the control. So, the frequency of cells with the modal number of chromosomes being equal to 50 decreases significantly, and the range of variability in the number of chromosomes increases. With the prolongation of the term of cultivation in control variants up to 60 days the character of cell distribution for chromosomal number changes, as compared to the preceding terms (15 and 30 days), which is expressed in the extended range of variability in the chromosomal number at the expense of decreased frequency of cells with submodal number of chromosomes equal to 49. But the degree of these changes is significantly smaller than in contaminated variants. The frequency of polyploid cells did not differ in all investigated variants. The number of chromosomal aberrations in cultures contaminated with A. laidlawii (for 15-60 days) and M. arginini (for 30 days) does not differ from that in the corresponding controls. The absence of dicentrics (telomeric association) at a long-term contamination of the human epithelioid cervix carcinoma cell line M HeLa clone 11 having marker chromosomes in karyotype and a comparison of these results with the earlier obtained data on other "marker" and "markerless" cell lines seems to confirm the point of view that dicentrics appear a characteristic feature of karyotypic variability of "markerless" cell lines, mainly with a long-term contamination in different conditions.

HeLa Cells↗

PubMed1999

[Characteristics of quantitative variability of karyotype in cell line of fibroblasts from indian muntjac].

The numerical regularities of karyotypic variability in cell line of the Indian muntjac skin fibroblasts (Muntiacus muntjak) has been studied. It was found that the karyotypic structure of cell population is mainly determined by some number of specific variant deviations from the main structural variant of karyotype (MSVK) to be depended on internal connections between chromosomes. Specific regulations determining the karyotypic structure of cell population are: 1) nonrandom character of cell distribution according to the number of chromosomal deviations from MSVK; 2) specific character of deviations in each chromosome from MSVK; 3) presence of significant connections between individual chromosomes by simultaneous mainly single directed numeral deviations. Results presented in this investigation were thought owner by analysing the number of individual chromosomes. These results extend considerably the known ideas of regulations of karyotypic variability in cell populations in vitro.

Animals↗

PubMed1999

[Characteristics of quantitative karyotypic variability in cell line of kidney from rat kangaroo (Potorous tridactylis)].

The goal of this work was to investigate the numeral karyotypic variability in different sublines (MT, M2). These sublines are formed spontaneously from the main cell line (M) and have modal number of chromosomes 9 and 10, MSVK (main structural variant karyotype)--3 + 3 + 1 + 2 and 3 + 4 + 2 + 1. There are general regulations which were originally got for the line M. In particular: 1) nonrandom character of cell distribution according to the number of chromosomal deviations from MSVK; 2) specific character of deviations of each chromosome from MSVK; 3) presence of significant connections between separate chromosomes by simultaneous, mainly single directed numeral deviations. These three lines (M, MT, M2) were compared and some differences were found: 1) different frequencies of deviations from MSVK; 2) the same chromosomes have tendency to different numeral deviation; 3) the specificity of some significant connections between separate chromosomes by simultaneous numeral deviations. These results lead us to a conclusion that the balance of numerical karyotypic structure in cell populations depends on the regulations connected with the character of deviations according to the number of chromosomes from MSVK which has the largest selected advantage. Each line has its own specific limits of karyotypic variability.

Animals↗

PubMed1999

[The study of quantitative karyotypic variability by induction of chromosomal instability in cultured cells of the Indian muntjac skin fibroblasts].

The influence of mycoplasmal contamination and somatic cell hybridization on the character of karyotypic variability in cell cultures of Indian muntjac skin fibroblasts has been investigated. Mycoplasma arginini and Acholeplasma laidlawii, used as factors inducing chromosomal instability, do not break the main regulations peculiar to intact control. They regulations are: 1) nonrandom character of cell distribution according to the number of chromosomal deviations from MSVK; 2) specific character of deviations of each chromosome from MSVK; 3) presence of significant connections between separate chromosomes by simultaneous mainly single directed numeral deviations. However, mycoplasmal contamination promotes the increase in the number of deviations in the direction of a decreasing chromosomes number. There is a breach of some connections between chromosomes by simultaneous deviations. They are chromosomes with broken connections according to the number of deviations which form telomeric associations (dicentrics). The number of these associations excel essentially intact control. The formation of new MSVK in subline M2 cells of the Indian muntjac in the process of chromosomal segregation in cell hybrid (M2 x clone of JF1 rat Jensen sarcoma) depends on the presence of significant connections between chromosomes by simultaneous numerical deviations in direction of MSVK formation. They are chromosomes that take part in the formation of new MSVK which form telomeric associations. These associations can be observed till stabilization of new MSVK. Probably, the support of the balance of karyotypic structure by factors inducing chromosomal instability is connected with change of some connections between chromosomes according to the number by simultaneous deviations as well as with the formation of dicentrics.

Acholeplasma↗

PubMed1999

[Characteristics of quantitative karyotypic variability in cell line of kidney from rat kangaroo (Potorous tridactylis)].

The numerical regulations of karyotypic variability in cell line of rat kangaroo kidney, NBL-3-11, has been investigated. These regulations are similar with ones found for cell lines of the Indian muntjac skin fibroblasts (M, MT, M2). In particular the balanced karyotypic structure of cell population in vitro is determined by correlations of the structural variants of the karyotype (SVK). These correlations depend on the following regulations 1) nonrandom character of cell distribution according to the number of chromosomal deviations from MSVK; 2) specific character of deviations of each chromosome from MSVK; 3) presence of significant connections between separate chromosomes with simultaneous numeral deviations some differences in the character of significant connections between the individual chromosomes. These connections have either single directed character, mainly (+) direction, or differently directed one by deviations of each chromosome mainly in one direction in cell line NBL-3-11. At the same time single directed character of simultaneous deviations is observed in cell lines of the Indian muntjac skin fibroblasts (M, MT, M2) either in (+) or (-) direction. Represented results confirm and extend considerably the known ideas of the regulations of karyotypic variability in cell populations in vitro.

Animals↗

PubMed1998

[Effect of mycoplasma contamination of the human uterine leiomyosarcoma cell line SK-UT-1B on karyotype structure].

The karyotypic variability has been investigated for human uterine leiomyosarcoma cell line SK-UT-1B, cultivated for 30-90 days after contamination with Acholeplasma laidlawii, strain PG-8. The character of cell distribution for chromosome number gradually changes in contaminated cells, comparatively to the control, with the lengthening of the term of contamination. So, in 30 days the analysed distributions do not differ in the experimental and in the control variants, the modal number of chromosomes being equal to 46. In 60 days the frequency of cells with modal number of chromosomes have a tendency to decrease, and the range of variability in the number of chromosomes tend to increase. In 90 days, the frequency of cells with modal number of chromosomes decreases significantly, and the range of variability on the number of chromosomes increases significantly. The number of chromosomal aberrations gradually increases in contaminated cells, as compared to the control, with the lengthening of the term of contamination. So, in 30 days the number of chromosomal aberrations does not increase, only the number of dicentrics (telomeric associations) has the tendency to increase. In 60 days, the number of chromosomal aberrations, mainly dicentrics, increases significantly. In 90 days, the number of chromosomal aberrations increases significantly, including both dicentrics and chromatid breaks. The possible reasons of the observed character of karyotypic variability is discussed. Our previous results make it possible to suppose that the increase in the number of dicentrics in "markerless" line SK-UT-1B with long term contamination may be an additional evidence on the role of dicentrics in cell adaptation to in vitro conditions in such lines.

Acholeplasma laidlawii↗

PubMed1997

[Effect of an extracellular heat shock protein on chromosome variability in Indian muntjak cultured skin fibroblasts].

It is known that the essential function of extracellular HSP70 (e-HSP70) to protect cell processes, indirectly associated with the genetic structures. A direct influence of e-HSP70 on chromosomal stability has not been studied. This explains actuality of the suggested investigation. A study was made of the influence of e-HSP70 on chromosomal variability at different phases of the first mitotic cycle in both intact and ciprofloxacin (CF) treated cells of the Indian muntjac skin fibroblasts. E-HSP70 (10 mg/ml) exerts no influence on the level of chromosomal aberrations, typical of the control. After a joint action of e-HSP70 and CF (50 mg/ml) no influence was also exerted on the antibiotic induced genotoxicity effect. CF and e-HSP70 (50 and 100 mg/ml, resp.) acting apart on intact cells during 6 and 24 h induce a significant increase of chromosomal aberrations compared to the control, primarily at the expense of chromatid or chromosomal breaks (depending on the duration of respective effect). CF and e-HSP70 acting apart on intact cells during 6 h with the following cultivation for 18 h in the fresh medium prior to fixation also induce a significant increase of chromosomal aberrations compared to the control, primarily at the expense of both breaks and dicentrics (telomeric associations). The joint action of CF and e-HSP70 on cultivated cells during 6 and 24 h and when CF and e-HSP70 were added respectively on 24 and 6 h prior to fixation, a significant decrease in chromosomal aberrations compared to the control level was induced. A simultaneous addition of CF and e-HSP70 in 6 h with the following cultivation for 18 h in fresh medium prior to fixation exerted no influence on the degree of genotoxicity effect, typical of the separate action of these agents. However a significant decrease in the number of dicentrics occurred. Apparently, e-HSP70 has a protective effect on chromosomal stability mainly at phase 62 of the mitotic cycle. The denaturated e-HSP70 (50, 100 mg/ml) has a genotoxicity effect similar to that of the infact e-HSP70 under above conditions. The joint action of CF and denaturated e-HSP70 (50 mg/ml) during 24 h exerts no influence on the degree of genotoxicity effect, induced by the separate action of these agents or leads to an increase in the number of dicentrics, acting separately or jointly compared to the control. The denaturated e-HSP70 (100 mg/ml) acting jointly with CF for 24 h, increases the degree of genotoxicity effects, induced by separate actions of the agents. Lipopolysacharide (50 mg/ml) exerts no influence on the number of chromosomal aberrations in the control. The sensitivity of individual chromosomes and their regions to agents inducing chromosomal instability is different. The preferential involvement of some chromosomes in dicentric formation was observed. A possible role of dicentrics in adaptation of cells belonging to "markerless" lines to infavourable factors of the environment, and possible mechanisms of protecting effect exerted by e-HSP70 on chromosomes are discussed.

Animals↗

PubMed1996

[The effect of the Mycoplasma contamination of 2 kidney cell sublines from the kangaroo rat on their karyotypic structure].

The karyotypic variability has been investigated for two cell sublines of Rat kangaroo kidney cultured for 40-160 days after contamination with Acholeplasma laidlawii, strain PG-8. The contaminated cultures did not differ from non-contaminated ones in cell distribution for chromosome number. The majority of cells of subline NBL-3-11 with modal number of chromosomes displayed the main structural variant of the karyotype (SVK)--2+2+2+2+2+1; in subline NBL-3-17 the main SVK being 3+3+3+3+3+2. A comparison of intact cultures of these sublines in cell distribution for chromosome number show just the opposite direction of aneuploidy processes: cell heterogeneity for chromosome number decreased in NBL-3-11 and increased in NBL-3-17. The quantity of chromosomal aberrations, primarily chromosomal breaks, increases within 40-160 days of cultivation of contaminated cells of subline NBL-3-11. The number of chromosomal aberrations, mainly at the expense of dicentrics due to telomeric associations, increases after 40 days of cultivation of subline NBL-3-17 contaminated cells. During a long-term cultivation (110 days) of subline NBL-3-17 intact cells, there is an increase in the number of chromosomal aberrations, mainly dicentrics, whereas the extent of chromosomal breaks appears much less. The present results and other additional experimental data make it possible to suppose that the increase in chromosomal instability seen in subline NBL-3-17 at a long-term cultivation may be characteristic of this culture, in distinction to subline NBL-3-11. The most frequent breaks were seen in chromosomes 1, 2 and X of intact and contaminated cells in both the sublines. Chromosomes 1, 2 and 4 are mainly involved in dicentric formations by q (long) arms. The role of dicentrics in cell adaptation to in vitro conditions is discussed.

Acholeplasma laidlawii↗

PubMed1996

[The genotoxic effect of ciprofloxacin on cultured cells from the kangaroo rat kidney and on skin fibroblasts from the Indian muntjac].

The genotoxicity of an antibiotic ciprofloxacin (CF) in doses of 10, 25, 50 and 100 mkg/ml under its short-term (6-48 h) and long-term (15-30 days) action on sublines of Rat kangaroo kidney, NBL-3-11, and Indian muntjak skin fibroblasts has been studied. The emergence of genotoxic effect depends on the dose and time of ciprofloxacin action on both the sublines, but the degree of this effect does not depend on these parameters directly. Ciprofloxacin exerts no influence on cell distribution for chromosome number in subline NBL-3-11, and increases heterogeneity of this parameter in the subline of Indian muntjac skin fibroblasts in 30 days after its addition in doses of 25 and 50 mkg/ml. The degree of increase of chromosomal aberrations in the subline of Indian muntjak skin fibroblasts was in average 1.5 times more than in NBL-3-11 in all examined variants compared to the control. The minimum antibiotic dose that induced chromosomal aberrations was 25 mkg/ml in the subline of NBL-3-11 under a short-term action and 50 mkg/ml under a long-term action. For the subline of Indian muntjac skin fibroblasts the minimum inducing dose was 50 mkg/ml irrespective of the duration of action, except the case of 15 days, when the number of dicentrics increased still at 25 mkg/ml. In both sublines with the duration of ciprofloxacin action within 6-24 h the replacement of chromatid aberrations by chromosomal aberrations occurred. Under a long-term ciprofloxan action differences in types of chromosomal aberrations were discovered: for subline NBL-3-11 these were mainly chromosomal breaks; in the case of muntjac cells both chromosomal breaks and dicentrics (telomeric associations) occurred. The preferential involvement of some chromosomes in dicentric formation was observed. In cells of the muntjac subline, unlike NBL-3-11, the sensitivity of individual chromosomes to ciprofloxacin-induced breaks differed from that to spontaneous breaks. In both the sublines ciprofloxacin induces chromosomal breaks mainly in definite regions of chromosomes. Possible reasons of differences between the examined sublines towards the character of chromosomal instability are discussed in addition to the role of dicentrics as a proposed adaptation of cells to unfavourable factors of the environment.

Animals↗

PubMed1994

[A cytogenetic study of the Chinese hamster lung cell line V-79 infected with Mycoplasma arginini and decontaminated using ciprofloxacin].

Karyotypic variability has been studied in a line of the Chinese hamster cells artificially contaminated with Mycoplasma arginini. The contaminated cultures differed from mycoplasma-free cells in cell distribution for chromosome number. The frequency of cells with modal chromosome number 21 decreased, while that of cells with 20 chromosomes increased. Decontamination of cell culture with ciprofloxacin (10 mg/ml) and a subsequent cultivation of cell in the antibiotic-free medium did not restore the original cell distribution for chromosome number. In-53--131 days after infection, the increased frequency of chromosomal aberrations was registered. Eradication of Mycoplasma by ciprofloxacin and a long-term cultivation in antibiotic-free medium restored the frequency of chromosomal aberrations to the control level corresponding to mycoplasma-free cultures. In contaminated cultures no cyto- or genotoxic effect of ciprofloxacin was observed. These data, together with the previous ones, enable the authors to recommend ciprofloxacin to make cell cultures free from mycoplasmas with minimal risk to change the original properties of cell lines.

Animals↗

PubMed1994

[The effect of mycoplasmal contamination of cultures of skin fibroblasts from the Indian muntjac and of the subsequent decontamination of the cultures using ciprofloxacin on the karyotypic structure of the cell line].

The karyotypic variability of Indian muntjac skin fibroblast cell line, cultured for 95-168 days after contamination with Acholeplasma laidlawii strain PG-8, has been investigated. The contaminated cultures differ from noncontaminated ones in cell distribution for chromosome number. The noncontaminated cultures have modal number of chromosomes equal to 7 with the main structure variant of the karyotype (SVK) 2+2+1+1+1. In the contaminated cultures the cell number with 7 chromosomes and the main SVK 2+2+1+1+1 decreased, whereas the cell number with 6 chromosomes increased along with the main SVK 2+2+1+1 resulting from the loss of chromosome Y1. The treatment of cells with ciprofloxacin for mycoplasma decontamination did not restore the normal cell distribution for chromosome number. The frequency of chromosomal aberrations, mainly dicentrics, due to telomeric associations, increased after 95-168 days of cultivation of contaminated cells. Chromosomes 1 and 2 and their combination are mainly involved in dicentric formations. The treatment of contaminated cells with ciprofloxacin restores the initial frequency of chromosomal aberrations. Chromosomes with altered structures have not been demonstrated. It has been shown that cells became mycoplasma-free after 15 days of treatment with ciprofloxacin. The role of aneuploidy and dicentrics in cell adaptation to culture conditions is discussed.

Acholeplasma laidlawii↗