New substrates for the fluorometric determination of oxidative enzymes.
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Biomedical subjects
Publications and source records attributed to G G Guilbault.
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Enzymatic electrodes are described for the specific and sensitive determination of glucose in blood. The probes are based on the conversion of B-D-glucose by glucose oxidase chemically attached to the surface of a Pt electrode. The peroxide liberated is sensed by measurement of the current generated at +0.7 volts vs. S.C.E. The enzyme, chemically attached to pig intestine via glutaraldehyde coupling is quite stable for months, even at room temperature. Other glucose electrodes developed at the University of New Orleans, using Pt (measurement of the O2 consumed at - 0.7 volts vs. S.C.E.) and I- probes are also described.