Search PubMed⌕ Search

Biomedical subjects

G Friedman

Publications and source records attributed to G Friedman.

At least 163 records · Page 9Linked to original sources
PubMed1978

Lipoprotein lipase of cultured mesenchymal rat heart cells. III. Effect of glucocorticoids and insulin on enzyme formation.

Lipoprotein lipase activity was studied in mesenchymal cells isolated from rat hearts and cultured for up to 8 days. The enzyme activity increased markedly between day 3 and 5 while the subsequent increase was less pronounced. Addition of hydrocortisone to complete culture medium resulted in an increase in lipoprotein lipase activity at all stages of culture. Lipoprotein lipase activity did not increase after addition of insulin to the complete culture medium. In the presence of serum-poor medium between day 3 and 6, the increase in lipoprotein lipase activity was much lower than in the presence of complete culture medium. Addition of hydrocortisone and insulin to the serum-poor medium resulted in a significant rise in lipoprotein lipase activity while less consistent effects were obtained after addition of each hormone alone. Transfer of cells to serum-poor medium between day 6 and 7 of culture caused a fall in enzyme activity. Addition of hydrocortisone alone and with insulin restored enzyme activity to control values. No effect on lipoprotein lipase was seen with estradiol, growth hormone, or glucagon when added to serum-containing medium, or serum-poor medium. These results indicate that the lipoprotein lipase of heart is controlled by glucocorticoids and that this control might require the presence of insulin for optimal expression.

Animals↗
PubMed1978

Acute lymphoblastic leukemia. Its occurrence with hypereosinophilic syndrome' and bilateral spontaneous pneumothorax.

A patient with acute lymphoblastic leukemia had an unusual prodrome, including clinical features of "hypereosinophilic syndrome," pulmonary infiltrates, and bilateral spontaneous pneumothorax, which preceded the onset of leukemia by four months. The mechanism for the production of eosinophilia may have been related to the production of eosinopoietic factors by the leukemic cells.

Adolescent↗
PubMed1978

Study of cultured skin fibroblasts from patients with and without ischemic heart disease. Metabolism of low density lipoprotein and cholesterol ester, synthesis of cellular lipids and effect of chloroquine on accumulation of cholesterol ester.

The aim of the present study was to determine whether skin fibroblasts derived from patients with ischemic heart disease (IHD), which could not be related to accepted risk factors, would show a metabolic abnormality with respect to lipid or lipoprotein metabolism. Male patients 30-52 years old suffering from IHD were subdivided into two groups: those in whom IHD was not associated with risk factors such as hypertension, hyperlipoproteinemia, diabetes or smoking (group I); and those in whom heavy smoking was the only major risk factor recognized (group II). The controls were patients with angiographically normal coronary arteries (group III). Skin fibroblasts obtained from these patients were cultured and investigated with respect to metabolism of low density lipoprotein (LDL), synthesis of cellular lipids and induction of cholesterol ester accumulation in the presence of chloroquine, an inhibitor of lysosomal hydrolases. After 24 h incubation, the uptake and degradation of LDL protein in cells from patients of group II was significantly higher than in the controls, group III, but not different from those of group I. Hydrolysis of [3H] cholesterol linoleate, and incorporation of [3H] oleic acid into total lipids and into cholesterol esters was similar in cell cultures of the 3 groups studied. After exposure to chloroquine and LDL, the cells from the different donors accumulated cholesterol ester to a similar extent. Thus, whereas no significant difference was encountered in the lipid and lipoprotein metabolism in cells of patients with IHD without risk factors and controls, some increase in LDL metabolism was seen in cells from patients with IHD and with a history of smoking. It remains to be determined whether this increase was causally related to smoking.

Adult↗
PubMed1978

Sinus bradycardia in the aged.

In the aged, sinus bradycardia represents the sinus-node-dysfunction component of diffuse conduction system disease associated with coronary and cerebral arteriosclerosis. Findings are presented on 15 patients whose ages ranged from 69 to 93 years at the time of admission to a home for the aged. They remained under observation (with electrocardiographic data) for from less than 1 year up to 18 years. The findings illustrate the evolution of conduction system abnormalities, including sinus node dysfunction, occurring either as an initial or an interval event. These abnormalities represent positive indications for pacemaker insertion in the aged.

Aged↗
PubMed1977

Cerebrospinal fluid lactic acid levels in meningitis.

Measurement of cerebrospinal fluid lactic acid by gas liquid chromatography and by an enzymatic Monotest lactate test was evaluated for the early detection of bacterial meningitis in 396 patients. Spinal fluid specimens from 62/62 patients with a bacterial or mycoplasma etiology yielded lactate levels greater than the upper limits of normal, whereas specimens from 334 patients with no bacterial involvement gave values within the normal range. The duration of elevated CSF lactate values coincided with the clinical response to therapy. When considered along with the history and physical examination of the patient, determination of lactic acid proved to be a rapid and reliable diagnostic test for the early detection of untreated as well as partially treated pyogenic meningitis.

Adolescent↗
PubMed1977

Treatment of acute hepatic encephalopathy with L-dopa.

A clinical report of a 30-year-old woman who developed acute hepatic failure in the fifth month of pregnancy is presented. L-dopa administration resulted in a marked improvement in both level of consciousness and electroencephalogram. The literature dealing with L-dopa therapy in hepatic encephalopathy is reviewed.

Adult↗
PubMed1977

In vitro inhibition of human peripheral blood lymphocyte transformation by an extract of Pseudomonas putida.

An extract prepared from a psychrophilic strain of Pseudomonas putida was found to cause a dose-dependent inhibition of [H3]TdR incorporation into human peripheral blood lymphocytes stimulated with PHA, ConA, PWM, or in a mixed lymphocyte reaction. The inhibition was found not to be the result of cytotoxicity, culture medium depletion of a component necessary for lymphocyte transformation, or interference with label uptake by blast lymphocytes. The extract was most effective when added prior to 48 h of mitogen stimulation. The inhibitory material was photeolytic enzyme degradable, heat-stable and non-dialyzable.

Cell Extracts↗
PubMed1976

Deposition and mobilization of cholesterol ester in cultured human skin fibroblasts.

Human skin fibroblasts in culture served as a model system to study intracellular cholesterol ester deposition in mesenchymal cells. Confluent cultures were exposed to homologous low density lipoprotein alone and together with chloroquine. In the presence of low density lipoprotein alone, even at half circulating serum concentrations, cellular free cholesterol increased no more than 12%, while the increase in cholesterol ester ranged from 13--100% during 48 h of incubation. Addition of chloroquine to the culture medium containing low density lipoprotein resulted in a very marked increase in cholesterol ester and the ratio of cellular esterified cholesterol to free cholesterol rose up to 2.2. In the presence of chloroquine the sum of uptake and degradation of 125I-labeled low density lipoprotein was enhanced and at higher chloroquine concentrations the more pronounced inhibition of degradation resulted in the intracellular retention of undegraded protein. Upon removal of the chloroquine-containing medium, there was a slight fall in the cellular cholesterol after 24 h incubation in a medium containing 10% fetal calf serum. Replacement of the fetal calf serum by lipoprotein-deficient serum and a mixture of high density apolipoprotein and sphingomyelin increased very significantly the loss of total cholesterol from the cells. At the same time the rate of loss of the retained labeled low density lipoprotein was also increased. The present results provide an adequate and reproducible model system for the study of cholesterol accumulation in human mesenchymal cells, which is one of the basic changes in atheromatosis. The availability of cholesterol ester laden cells also provides a good system for the study of agents active in cholesterol removal.

Adult↗
PubMed1976

Non-invasive quantitation of corneal copper in hepatolenticular degeneration (Wilson's disease).

The corneal copper content was measured by X-ray excitation spectrometry in two controls and in seven patients with Wilson's disease. Patients who were treated irregularly or not treated at all showed a high corneal copper content. Those who were adequately treated had low levels, comparable to the controls. In one case the corneal copper content declined 45%, after a course of dimercaprol. The corneal copper measured showed no correlation with the slit-lamp appearance of the Kayser-Fleischer ring. It is suggested that non-invasive X-ray excitation spectrometry can provide a fast and reliable method for the early diagnosis of Wilson's disease and for the objective evaluation of the efficacy of the treatment of this disease.

Adolescent↗
PubMed1976

A complex case of sarcoidosis fever.

Febrile episodes in a male patient with coronary artery disease and arteriovenous malformation of the spinal cord had been attributed to urinary-tract infections and pulmonary atelectasis. The autopsy findings, however, indicated that the fever may have been related to sarcoidosis which had not been detected pre mortem.

Aged↗
PubMed1976

Systemic candidiasis and endophthalmitis in neurosurgical patients. Report of three cases.

Three cases of systemic candidiasis in neurosurgical patients are presented. Two of the three patients also had endophthalmitis. All of the cases were treated with broad-spectrum antibiotics and glucocorticoids prior to the appearance of candidiasis. The authors stress the high susceptibility of neurosurgical patients to opportunistic infections of this type.

Adult↗
PubMed1976

An evaluation of the safety of a low-saturated-fat, low-cholesterol diet beginning in infancy.

This study was designed to detect developmental, body chemistry, or somatic differences that might be present in children who had been on a low-saturated-fat, low-cholesterol (LSFLC) diet since birth. Comparison to a matched control group at age 3 years failed to demonstrate significant differences in percentile height, percentile weight, head circumference, age, skinfold thickness, total serum protein, hemoglobin, number of failures on a Denver Developmental Screening test, or number of sick visits. Experimental children did have significantly lower serum cholesterol than a large group of children of similar age. It is concluded that at age 3 years, no significant adverse findings were present in the group who had been nourished on a LSFLC diet since birth as compared to a control group.

Arizona↗