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Biomedical subjects

G Flandrin

Publications and source records attributed to G Flandrin.

At least 19 recordsLinked to original sources
PubMed1992

Use of the new Coulter MAXM for leucocyte differentials.

The Coulter MAXM is an automated instrument designed to give a complete haematological profile including cell counts and WBC differential (DIFF). The WBC DIFF is determined by tridimensional flow cytometry on the basis of cell volume, light scattering and conductivity (VCS technology). Evaluation of the MAXM DIFF was performed by comparison with microscopic examination of blood smear. A total of 467 samples were studied by the MAXM and reference techniques, according to a standard protocol employed in our laboratory to define the criteria for smear examination. Good correlation was obtained between the two methods in non-flagged samples and those flagged only with an isolated "Imm Gran" message. In a group of 407 non-haematological and 60 haematological patients, there were 12 (2.6%) false positive and 5 (1.1%) false negative reports, all in non-haematological cases and the five false negative results corresponding to no more than minor abnormalities. The Coulter MAXM was thus shown to be an excellent haematology analyser. This instrument was capable of detecting significant abnormalities of blood smears with a sensitivity of 97.9%, a specificity of 94.7% and a global efficiency of 96.3% in the samples examined in the present study.

Automation↗
PubMed1979

[Bone lesions in hairy cell leukaemia (author's transl)].

Between 1959 and 1978, 150 patients with tricholeucocytic leukaemia were seen, 5 having radiological bone lesions. This has been reported only once in the literature, its significance and therapeutic consequences remaining ill-defined. One of the commonest sites of bone involvement is the neck of the femur. The usual appearances are those of demineralisation, lysis and pathological fracture. The true prevalence of such lesions could be determined only by routine studies for the presence of such lesions.

Adult↗
PubMed1979

[A new variety of acute non-promyelocytic leukemia with t(15;17)].

Three cases of a new variety of acute leukemia have been reported. The main features were: hyperleukocytosis made of large-sized blasts with a double shaped nucleus, few or no granulations in the cytoplasm, and in a few cell faggots or unique Auer rods; mycloperoxydase reaction was positive. This feature was associated with disseminated intravascular coagulation syndrome and t(15;17)(q22;q21) translocation in the majority of mitoses.

Acute Disease↗
PubMed1979

Leukemic phase of malignant histiocytosis (arguments in favour of the histiomonocytic origin of the abnormal cells).

A 15-year-old female was treated for malignant histiocytosis. The occurrence of a leukemic phase (178,000 blasts/cu mm) during the follow up provided the opportunity of studying a large number of malignant cells by cytochemical electron microscopic, and cell membrane markers techniques. Acid phosphatase reaction was strongly positive and totally inhibited by tartaric acid. Nonspecific esterase reaction was moderately positive with inhibition by sodium fluorid. Electron microscopy revealed the presence of surface membrane pseudopods and the phagocytic activity of the cells. The leukemic cells had a receptor for the Fc fragment of IgG. These findings support the histiomonocytic origin of the abnormal cells in malignant histiocytosis.

Adolescent↗
PubMed1979

A new translocation in Burkitt's tumor cells.

A t(8;22)(q24;q11) translocation was found in blood, bone marrow, and ascites cells from a European Burkitt's lymphoma. Cell surface markers were identified as monoclonal IgG. The relationship between these two unusual findings is questionable in this cytologically typical Burkitt's lymphoma.

Aged↗
PubMed1979

Erythrokinetic studies in hairy-cell leukaemia.

Erythrokinetics were studied in 29 patients with hairy-cell leukaemia. In all cases there was an increase in plasma volume, closely correlated to the size of the spleen, indicating that the true degree of anaemia can only be appreciated by red cell volume measurement. Moderately increased haemolysis was observed in most cases, which did not correlate with the spleen size. Simultaneous study of autologous and isologous red cell life-span suggested an extra-corpuscular mechanism for the haemolysis in most patients. A quantitative erythropoietic defect, either relative or absolute, was found in half the cases, without any qualitative defect. Only one case showed erythroid metaplasia of the spleen. Thus marrow failure appears to be largely responsible for the anaemia and granulocytopenia in hairy-cell leukaemia. A clear correlation was shown between the short-term prognosis after splenectomy and the degree of hypersplenism. However, long-term survival correlated chiefly with the degree of bone marrow failure, whether splenectomy had been carried out or not. The results indicate that isotope studies in hairy-cell leukaemia are useful both in determining the best form of treatment and predicting survival.

Adult↗
PubMed1979

[T (15;17) translocation in acute promyelocytic and acute nonpromyelocytic leukemia (author's transl)].

Seven acute promyelocytic leukemias (APL) were compared with three atypical acute myeloblastic leukemias (AML). These three AML were characterized by high hyperleukocytosis, mostly formed of monocytelike myeloblasts, disseminated intravascular coagulation syndrome, and a t (15;17) translocation in the majority of leukemic cell mitoses. This translocation was inconsistently found in typical APL defined as M3, according to the FAB classification.

Adolescent↗
PubMed1978

[Use of the LARC system in a laboratory specializing in hematology (author's transl)].

Two thousand consecutive white blood cell counts were simultaneously studied by the LARC system and the traditional manual method. This comparison enables the following four couclusions to be drawn.--The reproducibility of the LARC differential is superior to the traditional method;--The similarity between results obtained by the LARC and the manual method are good, as indicated by scatter-plots and calculated correlation coefficients;--The LARC system can be substituted for the traditional manual method, also for markedly pathologic samples, but in this case at the price of a slowing down of the through-put rate of the system;--The detection of abnormal white cell types is as good or better with the LARC system as compared with the manual method.

Autoanalysis↗