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Biomedical subjects

G Fischer

Publications and source records attributed to G Fischer.

At least 613 records · Page 34Linked to original sources
PubMed1975

[Studies on the distribution of glycogen phosphorylase and succinate dehydrogenase in the humeral epiphysis of various-age rats].

In the chondrocytes of the humerus epiphysis of newborn and 8-, 30- and 60-day-old albino rats, glycogen phosphorylase and succinate dehydrogenase activity was found. The enzymatic pattern of glycogen phosphorylase and succinate dehydrogenase diverges significantly within the age groups. In the chondrocytes of cartilage epiphysis of newborn and 8-day-old animals glycogen phosphorylase is distinctly present. The enzyme is reduced in 30- and 60-day-old rats. The initial moderate reactions of succinate dehydrogenase in the column cartilage and in the proximal hypertrophic cells demonstrate an additional activity from 30-day-old animals. The osteoblasts of the metaphysis demonstrated a strong reaction.

Age Factors↗
PubMed1975

[The influence of electrostatic fields on osmolality and concentrations of neutral fat and cholesterol (author's transl)].

Rats were exposed to electrostatic fields of different intensities and exposure times, after which the osmolality of the serum and the concentration of neutral fat and cholesterol in the serum were measured. The triglyceride and cholesterol concentrations of the sera were independent of the strength of the electrostatic field and independent of the length of exposure of the animals in the constant field or Faraday cages. They always lay within normal limits, but moved according to the conditions of the constant field: the neurtal fat concentrations to the upper, and the cholesterol concentrations to the lower limit. The differences compared to the controls could be confirmed statistically.

Animals↗
PubMed1975

[Immunization experiments for producing antibody-like substances in caterpillars of Mamestra brassicae L. (Insecta, Lepid., Noct.)].

The agglutinins against human blood cells described in caterpillars of Mamestra brassicae L. were not demonstrable when feeding the animals with a semisynthetic food. After injection or oral intake of certain bacteria (E. coli or streptococci of group C) or even Pope's broth the "antibody-like substances" known from feeding with natural food are being formed, and they agglutinated all human blood cells. The individual animals showed differences regarding the strength of agglutinin formation. The immune reactions observed possibly indicate the existence of a primitive immune system in these species (arthropods).

Agglutination↗
PubMed1975

Studies on the cultivation of Clostridium oncolyticum M 55. 5th communication: The influence of iron, zinc, and cobaltous ions on the growth and the kininase activity of clostridium oncolyticum M 55 ATCC 13.732.

The precipitation or iron sulfide by hydrogen sulfide excreting Clostridium oncolyticum M 55 led to further examinations in order to avoid this disturbing factor. The method should render the cultivation of Cl. oncolyticum M 55 under definite conditions and should also intensify the formation and the enrichment of the clostridial kininases. Cobaltous ions and zinc granules were found useful for cultivation, because no disturbances increased from both during sterilization and incubation. The determination of the growth cycles and the replication rates showed the usefulness of zinc for substitution of iron. Cobaltous ions did only allow a low replication rate. These results confirmed those showing the influence of chelate-forming agents on the kininase activity. Cobaltous ions were not able to exchange the central atom of the metallo-proteide. The influence of the peptone quality on the kininase activity in presence of zinc was also examined, since examinations with iron media have shown a direct connection between peptone quality and enzyme activity. The results with zinc-containing media were nearly 5-10% lower than those with iron. The results allowed also the conclusion that the kininase excreted by Cl. oncolyticum M 55 is an iron-containing metallo-proteide, whose central atom is exchangeable for zinc. The consequences of the loss of enzyme activity for the oncolytic therapy with Cl. oncolyticum M 55 will be shown in further examinations.

Acetates↗
PubMed1975

[Posterior osteosynthesis in the treatment of spinal cord tumors].

Prognosis of cancerous tumors of the spine depends above all on their neurological consequences. The authors have wondered whether osteosynthesis of the spine -- in addition to decompressive surgery -- can improve patient's survival and fonctional results. 23 patients, 20 with a metastatic tumor and 3 with a plasmocytoma were treated in this way. All of them had neurological deficits, 4 had isolated radicular symptoms, 1 a cauda equina syndrome and 18 a spinal cord compression (7 of which were complete). 20 of these patients suffered intractable pain of the spine. All the patients underwent decompressive surgical removal of tumor through laminectomy, associated with a bilateral posterior metallic osteosynthesis. The type of osteosynthesis consisted of two metallic plates placed along each articular column and screwed into the articular processes and the vertebral body through the pedicles of the adjacent normal vertebrae. Cobaltotherapy was added in 14 cases, hormonotherapy in one case and radioactive 131 1 in one another patient. Selective spinal angiography, performed in one case of thoracolumbar tumor was found useful in the location and avoidance of Adamkiedwicz's artery. With regard to the complication, there were two operative wound infections requiring reoperation. Our series -- in comparaison with others -- prove the effectiveness of the combined procedure. Patients without complete spinal deficit had a 20 months survival average. Patients with complete deficit had the same survival as in comparative group. But all the 20 patients suffering pain in the spine were substantially relieved. Such a combined procedure is indicated in cases of tumors involving the vertebrae, whether or not the epidural space is invaded especially when there are riskes of unstable fracture and only partial neurological deficits. The technique may be extended even to complete paraplegia because of its worth while antalgic effects. Thus this method -- by consolidating the spine -- relieving pain and consequently allowing kinesitherapy to be resumed earlier is able to improve the functionnal status and increase survival of the patients.

Adult↗
PubMed1975

[Proteinase-inhibitors and enzyme-active proteins from fish eggs. Fibrinagarelectrophoretic and immunoelectrophoretic studies (author's transl)].

Saline extracts from eggs and homogenized spermaplasma from 2 fresh water fishes and 2 sea-fishes were investigated using fibrin-agar-electrophoresis and the immunoelectrophoretic analysis. Many polyvalent proteinase-inhibitors with different inhibition spectra, lipoproteins and esterase active proteins which split beta-naphthylacetate as substrate were detected and designated according to a proposed nomenclature. Fresh water fishes contained more inhibitors than sea-fishes. The proteinase-inhibitor PI-Sg3 antigen-antibody complex was esterase active; the active complex designated PI-Sg4 could not yet be exactly coordinated in respect to another proteinase-inhibitor from Salmo gairdneri. The separation of pseudo- and euglobuline fractions permitted a significant concentration of proteinase-inhibitors and the elimination of large parts of strong antigenic proteins. A good antiserum could be prepared which showed 3-5 precipitation lines against proteinase-inhibitors from trout eggs. By means of cross reactions it could be demonstrated the nonspecificity of several proteins in respect to species, genus and sex. This concernes also some aspects of immunesera absorptions with heterologous protein mixtures. Cross reactions with proteinase-inhibitors couldn't be detected, suggesting that they are genus-specific. This is also confirmed by their variable number and inhibition-spectra in extracts of different origin.

Animals↗
PubMed1975

[Metabolism of rat liver in the electrostatic field and in the faraday cage before and after hepatectomy (author's transl)].

Investigations were performed with the aim of establishing the influence of various environmental conditions (such as steady field conditions, climatized laboratories, Faraday's cage) on a number of enzymic activities in the rat (including glutamic oxaloacetic tic transaminase, glutamic pyruvic transaminase, lactic dehydrogenase, gamma-glutamyl transpeptidase, acid phosphatase), as well as the serum concentrations of triglycerides, the oxygen consumption of hepatic parenchyma cells, and the influence on the incorporation of 3H-thymidine (following partial hepatectomy). In the steady field, the activities of the cytoplasmic enzymes (GOT, GPT, LDH) were higher then under Faraday conditions. The same applies both to the hepatic oxygen consumption and to the neutral fat serum levels. The control values always remained within the range of the results obtained under steady field or Faraday conditions. In the structure-linked enzymes (gamma-glutamyl transpeptidase, acid phosphatase) the results were not uniform. Following partial hepatectomy, and under steady field conditions, the serum triglyceride concentrations showed a less pronounced drop than they did in the controls. Under selected environmental conditions, the results obtained lie within the physiological range. The present findings, therefore, do not permit definite conclusions to be drawn on favourable or unfavourable effects exerted by the different types of electroclimates.

Acid Phosphatase↗