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Biomedical subjects

G F Sensabaugh

Publications and source records attributed to G F Sensabaugh.

At least 37 records · Page 2Linked to original sources
PubMed1985

Genetic relationships among Neisseria species assessed by comparative enzyme electrophoresis.

The electrophoretic mobilities of 12 enzymes from 19 Neisseria species (including 6 strains of N. perflava), Gemella haemolysans, Escherichia coli and Branhamella catarrhalis were characterized by polyacrylamide slab gel electrophoresis. All strains and species tested exhibited qualitatively different zymogram patterns. Species and strain relationships were quantified by pairwise comparisons of all 12 enzyme systems to obtain similarity indices; these data were subjected to numerical clustering methods to obtain groups and a phenogram. The electrophoretic classification compared favourably with those obtained by other criteria. In addition, the quantitative clustering data indicated that N. ovis and N. caviae are sufficiently different from the other Neisseria species to warrant their separation into a distinct group. These two species also lacked the characteristic NADPH-diaphorase zymogram pattern found in all the other Neisseria species. Intra-species similarity indices were generally greater than the inter-species index values. However, certain species such as N. meningitidis and N. gonorrhoeae had similarity index values in the range of inter-strain index values.

Dihydrolipoamide Dehydrogenase↗
PubMed1982

Flow permeation analysis of bovine cervical mucus.

Geometrical properties of the microstructure of whole bovine cervical mucus were studied. An experimental technique was developed for measuring the flow of fluid through the mucus microstructure in response to application or a prescribed external pressure gradient. The data obtained were analyzed in conjunction with a mathematical model of the hydrodynamics of the flow-permeation process. The sizes of typical interstices within the microstructure were calculated to be of the order of 1 micrometer, with typical macromolecular filament diameters being of the order of 100 A. These dimensions were interpreted as representative of an equivalent network giving rise to measured flow permeability. The values of filament size showed a strong experimental correlation with the solids content of the mucus.

Animals↗
PubMed1982

Hereditary plasma lecithin-cholesterol acyl transferase deficiency: a heterozygous variant with erythrocyte membrane abnormalities.

LCAT esterifies cholesterol to cholesterol ester. This study describes a family with plasma LCAT deficiency with a heterozygous variant. It was found that both parents and one son had half the normal plasma LCAT activity whereas their only daughter and other son exhibited zero plasma LCAT activity, suggesting an autosomal co-dominant inheritance of the enzyme deficiency. Erythrocyte membrane abnormalities (e.g., increased erythrocyte PC and cholesterol) that were associated with patients with zero plasma LCAT were also observed in family members with half the LCAT. Additionally, erythrocytes from LCAT-deficient individuals were more susceptible to peroxidant threat and had membranes that were more mechanically unstable than those from individuals with normal plasma LCAT activity.

Adult↗
PubMed1980

Genetic markers in semen. III: Alteration of phosphoglucomutase isozyme patterns in semen contaminated with saliva.

Contamination of semen by saliva can result in the alteration of seminal phosphoglucomutase (PGM1) isozyme patterns. The alteration is characterized by the gradual loss of the a and b isozyme bands the concomitant generation of anodal bands; eventually, all PGM activity is lost. The conversion of PGM isozyme patterns has been shown to be due to a dialyzable heat-labile factor in saliva and a nondialyzable heat-labile factor in semen. The implications of this conversion for PGM typing in sexual assault evidence are discussed.

Chemical Phenomena↗
PubMed1978

Phenotype dependence in the inhibition of red cell acid phosphatase (ACP) by folates.

Red cell acid phosphatase (ACP) is shown to be inhibited by folic acid and various folates. The degree of inhibition is phenotype dependent with a pattern of variation differing from that of the well recognized variation in red cell activity levels. The pattern of variation is ordered ACP1B less than ACP1A less than ACP1C in terms of the relative allelic contributions to the observed inhibition. This pattern correlates with previously observed patterns of risk for two hemolytic disorders and may thus provide a key to their understanding.

Acid Phosphatase↗
PubMed1977

Immunosuppressive activity of human seminal plasma. I. Inhibition of in vitro lymphocyte activation.

A high molecular weight fraction prepared from human seminal plasma by gel filtration chromatography suppresses human lymphocyte transformation and DNA synthesis induced by mitogens (PHA, Con A, PWM), antigens (Candida albicans, tetanus toxoid), and allogenic cells. This same fraction also suppresses the stimulated response of mouse lymphocytes to allogenic cells and to various mitogens, including T cell-dependent and T cell-independent mitogens. The induction, but not the expression, of cell-mediated cytotoxicity is also suppressed. Similar high molecular weight fractions suppress the in vitro humoral response of mouse spleen cells to both a T cell-dependent (SRBC) and a T cell-independent (DNP-F) antigen. The high m.w. fraction exhibited in vitro suppressive activity at concentrations of 0.1 to 1.0 mg/ml which corresponds to a 1/50 or greater dilution of human seminal plasma. These observations support the concept that a local immune response against sperm in the female reproductive tract is actively suppressed by a component in seminal plasma.

Animals↗
PubMed1976

Esterase D polymorphism in Chinese and Japanese.

Esterase D phenotypes have been determined in Chinese and Japanese populations in the San Francisco area. The EsD1 gene frequencies were 0.612 for the Chinese population and 0.582 for the Japanese population.

Asian People↗
PubMed1976

Sperm diaphorase: genetic polymorphism of a sperm-specific enzyme in man.

Human sperm contains en enzyme with diaphorase activity that appears to be unique to sperm. Electrophoretic analysis of the diaphorase activity in sperm of different individuals reveals three phenotypic patterns. This polymorphism can be explained in terms of two alleles segregating at an autosomal locus; the allele frequencies have been determined to be 0.71 and 0.29. This appears to be the first reported example of a sperm-specific genetic polymorphism in man; its existence raises a number of genetic and biochemical questions.

Biological Evolution↗