Biomedical subjects

G F Pinto

Publications and source records attributed to G F Pinto.

6 recordsLinked to original sources

PubMed1991

[General surgeon and breast reconstruction after mastectomy].

Breast reconstruction is widely used in patients who have undergone mastectomy for breast cancer. The paper underlines that reconstruction is often performed by the general surgeon who has become an expert in this type of plastic surgery. The Authors present their own clinical data, and describe the indications and techniques used, and the results obtained. In conclusion, it is stressed that ideally the same surgeon should be experienced in oncology and plastic surgery.

Adult↗

PubMed1991

Inhibition of horse liver alcohol dehydrogenase and rabbit muscle lactate dehydrogenase by phenylhydrazine.

The activity of horse liver alcohol dehydrogenase was inhibited by phenylhydrazine. Kinetic experiments showed that this compound produced linear competitive inhibition with respect to NAD+ and linear noncompetitive inhibition with respect to ethanol. These results suggested that the inhibitor competes with NAD+ for the coenzyme binding site of alcohol dehydrogenase, forming a dead-end complex with the free form of the enzyme. A Ki value of 393 +/- 51 microM was estimated for the enzyme-inhibitor complex. Further evidence for this mechanism of inhibition arose from the fact that the same kind of inhibition was found for rabbit muscle lactate dehydrogenase. The Ki value for the lactate dehydrogenase-phenylhydrazine complex was 43.41 +/- 2.10 mM. The significant difference between these Ki values is explained in terms of known differences in hydrophobicity of the nicotinamide binding region in the two enzymes.

Alcohol Dehydrogenase↗

PubMed1988

A microcomputer program for fitting two-substrate enzyme rate equations.

This paper describes a microcomputer program written in BASIC for fitting different two-substrate enzyme kinetic mechanisms. The present program is a complement of one which was recently published and was developed to fit different models of enzyme inhibition. The complete program resulting from this complementation allows the fitting of the most usual rate equations found in steady-state studies of enzyme kinetics. The program based on a non-linear least-squares regression, can be run on any microcomputer having the CP/M operating system. The initial rate equation for a steady-state random bisubstrate mechanism was chosen to evaluate the performance of the program, with contrived data of known error structure.

Computers↗

PubMed1987

A microcomputer program for fitting enzyme inhibition rate equations.

This paper presents a computer program written in BASIC for fitting different enzyme inhibition kinetic models. The program, based on a nonlinear least-squares regression, can be run on any microcomputer with the CP/M operating system. Weighting of observed initial velocities is decided by the user by assessing constant variance, proportional variance or by incorporation of the variances calculated by a subroutine. The program also uses robust regression by bisquare weighting. All questions concerning data input, type of rate function, type of weight and the use of bisquare regression appear on the video display unit.

Enzyme Inhibitors↗

PubMed1979

Application of a simple method to the characterization and differentiation of protein foods.

A screening method for the characterization and differentiation of proteinaceous samples and amino acid mixtures was applied to protein foods (4 protein-rich mixtures, one product sold as dietary supplement, and 3 raw materials of frequent use in the preparation of these products). Graphic profiles which describe the relative amounts of amino acid groups in the samples were obtained and subjected to statistical analysis. According to a previously established criterion of identity or difference, the correlation coefficients showed that at least 90% of the comparisons dealt with different samples. The method is proposed as a valuable tool for the quality control of protein-rich foods and their raw materials.

Amino Acids↗

PubMed1976

A screening method for protein characterization and differentiation.

A circular paper chromatographic method was developed for the separation of the amino acids in proteins into 7 subgroups. Butanol-acetic acid water (4+1+1) was used as the developing solvent. Eluted ninhydrin-stained aminograms gave rise to graphic profiles or numerical indexes based on absorbance percentages. The profiles can be used to compare protein-containing samples. Twenty different samples were studied through 190 comparisons of graphic profiles and coefficients of correlation, with only 4% misleading results. The method showed excellent reproducibility for the identification or differentiation of proteins and has the advantage of being performed with low-priced apparatus and reagents.

Amino Acids↗