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Biomedical subjects

G F Bahr

Publications and source records attributed to G F Bahr.

At least 55 records · Page 3Linked to original sources

Scanning electron microscopic observations of surface structure of isolated human chromosomes.

Isolated human chromosomes dried by the critical-point method have been assumed to retain their original three-dimensional shape when viewed under a transmission electron microscope. Our scanning electron microscopic study confirms this interpretation and reveals an appearance like that of a skein of yarn. The existence of fiber bridges between chromatid pairs and among chromosomes is demonstrated.

Chromosomes↗

Karyotyping of single human chromosomes from dry mass determined by electron microscopy.

Human chromosomes from cultures of peripheral lymphocytes were prepared for electron microscopy by surface spreading and critical-point drying. Total and partial determinations of chromosomal dry mass were made from contrast in electron microscopic negatives. The distribution of dry weights was analyzed with respect to the Denver-Chicago classifications. There is considerable variability of mass within each group, but no statistically significant differences between four donors. A single chromosome can be correctly classified from its total mass and the ratio of long- to short-arm mass.

Humans↗