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Biomedical subjects

G Enders

Publications and source records attributed to G Enders.

At least 91 records · Page 5Linked to original sources
PubMed1992

Persistence of specific IgM and low avidity specific IgG1 following primary rubella.

Persistence of specific IgM in sera following primary rubella infection was compared with the maturation of the specific IgG1 response. 206 sera, from 171 patients with primary rubella, taken 1 day to 2.5 years after onset of illness, were tested. Rubella-specific IgM was detected by M-antibody capture radioimmunoassay in 100% of sera taken 15-28 days after onset, but in only 9% taken 3-4 months after onset. However, using the diethylamine (DEA) shift value (DSV) method, low avidity specific IgG1 was detected in 91% sera taken at 3-4 months and at 5-7 months 21% of sera remained positive. Using an avidity index method, with urea in the wash buffer, none of the sera were positive for low avidity specific IgG1 beyond 3 months after onset. With DEA in the wash buffer, the number of sera positive rose to 38% at 3-4 months. Thus, the DSV method for detecting low avidity specific IgG1 is a useful additional test for confirming or refuting a diagnosis of primary rubella and is of particular value for assessing pregnant patients.

Antibodies, Viral↗
PubMed1992

Deposition of ozone to a mature spruce forest: measurements and comparison to models.

Removal of ozone at terrestrial surfaces provides a major sink for tropospheric ozone and, therefore, a constraint on the peak concentrations achieved during photochemical episodes. This study reports results from 5 years of almost continuous measurements of vertical profiles of ozone and related meteorological variables over a mature spruce forest in Bavaria. Deposition velocities calculated from flux/gradient and eddy correlation flux measurements have been compared with estimates based on a resistance model and yield satisfactory agreement during fine weather conditions. The results also suggest that biogenic emissions of reactive hydrocarbons from the forest influence the vertical profile of ozone.

Journal Article↗
PubMed1992

[Toxoplasmosis and important virus infections in pregnancy-- diagnosis and management].

Toxoplasmosis and viral infections such as rubella, cytomegaly and parvovirus B19 are much feared risk during pregnancy. The rate of connatal toxoplasmosis and of subclinically infected infants at birth with the risk of late manifestation is still unclear, whereas such data are fairly well-known for rubella-, CMV- and parvovirus-B19-infections. The respective major diagnostic issues in pregnancy, the laboratory diagnosis, and its rational use in combination with clinical information are presented. Also the value of passive prophylaxis, therapy, and prenatal diagnosis as well as the possible management for diminishing the infection problems in pregnancy are discussed.

Cytomegalovirus Infections↗
PubMed1990

Recognition of a hepatitis B virus nucleocapsid T-cell epitope expressed as a fusion protein with the subunit B of Escherichia coli heat labile enterotoxin in attenuated salmonellae.

Two overlapping T-cell sites of the nucleocapsid antigen (HBc) of Hepatitis B Virus (HBV) (amino acids (aa) 120-140) and a B-cell epitope of the pre-S(2) region of the HBV surface antigen (aa 133-140) were expressed as a fusion protein with the subunit B of Escherichia coli heat labile enterotoxin (LT-B) in attenuated salmonellae (aroA Salmonella dublin SL1438). When Balb/c (haplotype H-2d) mice were fed salmonellae expressing LT-B or the LT-B/HBV fusion protein they developed serum IgG anti-LT-B antibodies and splenic cells reactive to LT-B. C57BL/10 (H-2b), in contrast, showed anti-LT-B antibody titres, but no splenic cell priming to LT-B. Neither in Balb/c nor in C57BL/10 mice could an antibody response to the fused HBV antibody binding site be demonstrated. In C57BL/10, however, an HBc T-cell epitope fused to LT-B primed a splenic cell response to an analogous synthetic peptide (HBc aa 121-145) in four out of five mice after three oral immunizations. This is the first description of the priming of a cellular immune response to a defined heterologous epitope expressed in attenuated salmonellae and delivered by the oral route.

Animals↗
PubMed1990

Parvovirus B19 infections in pregnancy.

Since 1986, 2,279 pregnant women were screened for anti Parvovirus B19 IgG and IgM antibodies. Of them 41% were seronegative, 54% had specific IgG but no IgM antibodies, indicating an infection in earlier life. Acute infection could be demonstrated by IgM antibody detection in 114 pregnant women (32% in the first, 54% in the second and 14% in the third trimester). Hydrops fetalis occurred in 10 of the 114 pregnancies (8.7%). In five of these cases intrauterine exchange transfusion was done and until now four healthy babies were born. Three fetuses died and one pregnancy was terminated, and two pregnancies are not yet completed. Fetal loss occurred in 9 of the 114 pregnancies (7.8%) (three fetal deaths after hydrops fetalis in the second trimenon and six spontaneous abortions in the first trimenon). In 51 of the 70 pregnancies which came thus far to term clinical data were available from the newborns. All these children were healthy at birth and later. In 22 of the 51 newborns, serological investigation was possible. In only two of them evidence of prenatal infection was detectable.

Counterimmunoelectrophoresis↗
PubMed1990

Virus detection in the fetal tissue of a premature delivery with a congenital varicella syndrome. A case report.

Cases of congenital varicella syndrome have been published, to date, a single case reports. Isolation attempts of Varicella-Zoster virus from fetal tissues have, thus far, been unsuccessful. This is a first report of detection of Varicella-Zoster virus in fetal tissue by means of DNA hybridization technique in a typical case of congenital varicella syndrome in a premature delivery of the 27th gestational week. The case is documented anamnestically, sonographically, pathologically and virologically. In women with primary varicella infection during pregnancy good sonographical controls are recommended. In cases with sonographically characteristical signs prenatal diagnosis with puncture of the umbilical vein cord and placentocentesis may be considered. The varicella DNA detection should be supplemented, however, by the polymerase chain reaction.

Abnormalities, Multiple↗
PubMed1989

[The diagnosis of infections in pregnancy].

The frequent questions in which symptoms which infections should be excluded cannot be answered in a simple manner. In daily practise only such infections are possible to be excluded meaning a certain risk for the fetus. Of course other infections may be taken into consideration for differential diagnosis additionally. Especially Coxsackie-echoviruses, mycoplasmas and mononucleosis have been discussed as fetal damaging factors. Coxsackie-echovirus infections in late pregnancy may be responsible for severe neonatal diseases. Occasionally each banal infectious disease of a pregnant woman the gynaecologist is confronted with the question for the fetal risk. According to the symptoms the most important infections listed in tabl. XIV should be excluded. The remaining ones belong to the complex of the so called normal risk of 3.5 per cent in each pregnancy. This fact should be mentioned towards the pregnant women because of legal reasons.

Female↗
PubMed1988

[Suspected syphilis during pregnancy due to cross reactions in Borrelia infection].

A weakly positive titre (1:20) in the Treponema pallidum haemagglutination test and a highly positive titre (1:1280) in the fluorescence Treponema antibody absorption test, but negative result for IgM antibodies, were found in the serum of a 23-year-old pregnant woman. The cardiolipin microflocculation test was at first borderline positive, but negative on repeat. In the absence of a history of syphilis tests for Borrelia antibodies were performed. Those for antibodies against B. burgdorferi were highly positive in the ELISA test (550 units), in the indirect Borrelia immunofluorescence test 1:1280 for IgG antibodies and 1:160 for IgM antibodies. In the Borrelia-specific indirect haemagglutination test, which measures both IgG and IgM antibodies, the titres were 1:640 to 1:1280. These results confirmed the presence of an infection with B. burgdorferi and not with Treponema pallidum.

Adult↗
PubMed1988

Outcome of confirmed periconceptional maternal rubella.

61 pregnant women in whom confirmed rubella occurred from 5 weeks before to 6 weeks after the last menstrual period (LMP) were followed up prospectively. In 39, the pregnancy was terminated and the fetal tissues or mixed products of conception were examined for rubella virus. In 22, the pregnancy continued to term and cord serum was tested for specific IgM antibody. No evidence of intrauterine infection was found in 38 pregnancies in which the mother's rash appeared before, or within 11 days after, the last menstrual period. The shortest interval at which fetal infection occurred was when the rash appeared 12 days after the last menstrual period. All 10 pregnancies in which the rash appeared 3-6 weeks after the last menstrual period resulted in fetal infection: 4 of these pregnancies went to term, and all 4 infants were damaged. The risk to the fetus when rubella occurs before the mother's last menstrual period is probably negligible.

Female↗
PubMed1988

Congenital rubella infection after previous immunity of the mother.

A newborn boy was admitted with a congenital rubella infection. Seven years previously his mother had been vaccinated against rubella; 3 years previously rubella immunity had been confirmed. Therefore, intrauterine transmission must have occurred after maternal reinfection during pregnancy. Prenatal diagnosis of rubella embryopathy with serological methods after subclinical maternal reinfection is nearly impossible.

Adult↗
PubMed1988

Biological effects of shock waves: kidney damage by shock waves in dogs--dose dependence.

The effect of shock waves on normal canine kidneys was examined in three groups of dogs whose right kidneys were exposed to 500, 1500, or 3000 shock waves. Autopsy was performed 24-30 h later. The kidneys were enlarged with haemorrhages in the outer and inner renal capsule and intraparenchymally. Macroscopically intraparenchymal haemorrhages were restricted to the high pressure field of the shock wave and consisted of haematomas up to 18 mm diameter (most frequently 6 mm or less) and diffuse haemorrhages. Histologically, haemorrhages were shown to originate from interlobular and arcuate veins. Venous thrombosis, tubular dilatation, and diffuse interstitial haemorrhage occurred in the same area. The number of haematomas was larger, and diffuse haemorrhages were more extended after the application of 1500 and 3000 than after 500 shock waves. No difference was seen between 1500 and 3000 shock waves.

Animals↗
PubMed1988

Changes of immunoglobulin concentrations in the bile after proximal gastric vagotomy in rats.

We have investigated the influence of proximal gastric vagotomy in rats (PGV) on the immunoglobulin concentration in the serum, bile, and intestinal fluid. Clear differences for serum IgA were noted: after PGV, rats had 1.5 mg/ml IgA in contrast to only 0.25 mg/ml in sham-operated controls. The other serum immunoglobulins remained unchanged. Bile immunoglobulins were elevated in PGV rats with regard to IgA, IgG1, IgG2a, and IgG2b. In addition, PGV rats had higher IgA, IgG1, IgG2a, and IgG2b concentrations in the intestinal fluid than controls. An explanation for these high Ig concentrations in the secretions might be the challenge by intestinal (microbial) antigens and, perhaps, mucosal inflammation with changes in the permeability. Indications for the former were the increase in the number of bacteria after PGV.

Animals↗