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Biomedical subjects

G E Pierce

Publications and source records attributed to G E Pierce.

At least 109 records · Page 6Linked to original sources
PubMed1977

Correlation of spontaneous leukocyte blastogenesis with human renal allograft rejection.

Spontaneous blastogenesis of peripheral blood mononuclear cells in recipients of renal allografts was studied by incorporation of 3H-thymidine. Three different levels of reactivity were observed that correlated with the patient's clinical course. Low levels of blast activity (i.e., median 680 dpm/million cells with 95% confidence interval (CI) of 480 to 880 dpm) were observed in pretransplant patients. A moderate elevation (median 3,00 dpm with CI of 2,300 to 5,300 dpm) was noted post-transplantation during quiescent intervals and following the onset of acute reversed rejection. Marked elevations, i.e., values greater than 10,000 dpm frequently were observed within 6 days prior to rejections (median 21,000 with CI of 7,400 to 34,000 dpm) and during sustained rejections (median 14,000 with CI of 8,800 to 19,600 dpm).

Graft Rejection↗
PubMed1976

Organized kidney retrieval on a regional basis. A new approach.

A network of small to medium-sized communities has been developed to increase the supply of cadaveric kidneys for renal allotransplantation. After a training period, community physicians and the community hospitals were designated as members of the transplant team to retrieve kidneys. A threefold increase in the number of cadaveric kidneys has resulted from this project. This mechanism for organized kidney retrieval on a regional basis could be used to augment the supply of cadaveric organs in other areas of the United States.

Hospitals, Community↗
PubMed1975

Effects of human sera on reactivity of lymphocytes in microcytotoxicity assays.

The effects of cancer patient and normal donor serum samples on the reactivity of patient and normal lymphocytes against both normal and malignant target cells were studied in microcytotoxicity assays. There were 17 of 140 cancer patient serum samples and 7 of 116 normal donor lymphocyte samples that selectively increased the growth of target cells in the presence of lymphocytes. This effect was most often noted with cancer patient serum against cultured tumor cells, but the effect was also noted against fibroblasts and with normal serum against both fibroblasts and tumor cells. Of 140 cancer-patient serum samples, 11 selectively decreased target cell survival in the presence of lymphocytes compared to medium and compared to other serum samples. In the absence of lymphocytes these serum samples were nontoxic. The effect was not observed with any of the normal serum samples studied. The lymphocyte-dependent serum toxicity appeared to be selectively directed against tumor target cells.

Animals↗
PubMed1975

Microcytotoxicity assays of tumor immunity in patients with bronchogenic carcinoma correlated with clinical status.

Peripheral blood lymphocytes from patients with bronchogenic carcinoma were tested in microcytotoxicity assays against cultured bronchogenic cancer cells, other types of tumor cells, and skin fibroblasts. Lymphocytes from patients who were postresection with no clinical evidence of residual or recurrent tumor were more frequently toxic against bronchogenic carcinoma than were lymphocytes from normal donors or from patients with clinically evident disease. Lymphocytes from patients with minimal or no tumor were more frequently toxic against bronchogenic cancer than against skin fibroblasts. Serum samples from a few patients rendered lymphocytes toxic for bronchogenic cancer cells, but this serum activity could not be correlated with the patient's clinical status.

Adenocarcinoma↗
PubMed1975

The influence of protein concentration of the perfusate on weight gain and kidney transplant outcome.

We have described a technique to compare parameters of perfusion using canine kidneys. Using this system, we observed that weight gain during pulsatile perfusion varied inversely with perfusate albumin concentration. The weight gain was more rapid with albumin concentration below 4.0 Gm%. Perfusion characteristics deteriorated in association with early rapid weight gain, indicating greater ischemic damage to the kidneys perfused with the low protein perfusate. Finally, the protein concentration of cryoprecipitated plasms is low enough to cause a rapid increase in kidney weight, a condition predisposing to poor function upon re-implantation of the kidney.

Albumins↗
PubMed1975

Effects of human lymphocytes on cultured normal and malignant cells.

With a modified microcytotoxicity assay, the effects of lymphocytes from normal volunteers and from patients with a variety of solid malignant tumors were tested in vitro against both normal and neoplastic target cells. The study was divided into three sequential time phases in each of which a different method of lymphocyte separation was used. Lymphocytes were separated by a Ludox-polyvinyl-pyrrolidone technique in the first phase, by nylon wool filtration in the second phase, and by a Ficoll-Hypaque technique in the third phase of the study. In the first two phases of the study, lymphocytes from both normal volunteers and cancer patients were frequently toxic on both tumor cells and fibroblasts. In the last phase of the study types of target cells. Cancer patient lymphocytes were more frequently toxic on tumor cells but no more frequently toxic on fibroblasts than were normal lymphocytes. The different results obtained in the last phase of the study cannot be attributed solely to the different method of lymphocyte separation, since other factors, such as better growth status of the target cells and greater facility in performing the assays, might also be responsible. Results of the last phase of the study raise the possibility that both specific and nospecific reactions may contribute to the toxicity observed in this laboratory with cancer patient lymphocytes tested against tumor cells. It has not yet been possible to reduce the level or frequency of the nonspecific reactions to such a degree that the microcytotoxicity assay can be used clinically to document clearly or to follow the putative tumor-specific immunity of individual cancer patients.

ABO Blood-Group System↗
PubMed1974

Leukocyte adherence inhibition as measured by a radioisotopic technique for detection of cell-mediated tumor immunity.

It has been previously reported that cell-mediated tumor immunity may be detected by leukocyte adherence inhibition (LAI) as determined by a visual counting technique in the presence of soluble tumor antigen. In the present experiments leukocyte adherence was measured by a radioisotopic technique. Peripheral blood mononuclear cells from Wistar/Fu rats and lymph-node cells (LNC) from C3H/J mice were labelled with Technetium 99m (99mTc) and incubated with medium alone and with medium containing tumor antigens (high dilutions of dialyzed tumor homogenates) in the wells of plastic tissue-culture plates. After incubation, fluid and non-adherent cells were removed. The ratio of residual radioactivity in wells with antigen to that in wells without antigen was determined. Adherence of blood mononuclear cells from rats bearing syngeneic transplants of a polyoma-virus-induced tumor was inhibited by dialyzed homogenates of this tumor. Adherence of blood mononuclear cells from normal rats was not inhibited. Adherence of LNC from mice bearing syngeneic transplants of a 3-methylcholanthrene (3-MCA)-induced fibrosarcoma was inhibited by dialyzed homogenates of this tumor. Adherence of LNC from normal mice or from mice or from mice bearing a different 3-MCA-induced tumor was not inhibited. These results support the use of LAI as a simple test for cell-mediated tumor immunity. Measuring relative leukocyte adherence with the radioisotope 99mTc eliminates possible bias in counting and permits multiple replicate determinations with a small number of leukocytes.

Animals↗