The incidence of "leukovirus" in cultured human hematopoietic cells.
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Biomedical subjects
Publications and source records attributed to G E Moore.
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Several synthetic polymers (hydroxyethyl starch, sodium carboxymethyl cellulose, polyvinylpyrrolidone) markedly improved the growth of three human lymphocyte cell lines [Roswell Park Memorial Institute (RPMI) 1348, 1788, and 8098]. Growth was stimulated when each of these polymers was added to RPMI 1640 medium supplemented with only 2% fetal bovine serum. Dextran T-40, T-70, and T-110 varied in their effect on the growth of these cell lines. Dextran T-250 and Haemaccel did not improve cell yields when partially substituted for the serum. The successful partial substitution of polymers for serum was specific for individual cell lines.
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Permanent lymphocytoid cell lines have been established from patients with the XYY and XXY chromosome constitutions. Large amounts of these and other cell lines with genetic defects can be provided for various studies.