Search PubMed⌕ Search

Biomedical subjects

G Duverlie

Publications and source records attributed to G Duverlie.

26 records · Page 2Linked to original sources
PubMed1990

Latex enzyme immunoassay for measuring IgG antibodies to rubella virus.

A latex enzyme immunoassay (LEIA) for detecting IgG class antibody to rubella virus was compared with the latex agglutination test (LA) and a haemagglutination inhibition assay (HI). Of 243 sera tested, four discrepant results were observed among all three techniques, corresponding to borderline values. Except for one sample containing specific IgM class antibody, the difference between quantitative results from each pair of tests was always within a value corresponding to two doubling dilutions and was considered to have acceptable variation. The LEIA technique allowed 10 seroconversions to be detected, as determined by the other techniques. The LEIA required a single 1 in 8 sample dilution, took 30 minutes, and provided a useful alternative assay for the quantitation of rubella antibodies.

Antibodies, Viral↗
PubMed1989

Heterophile IgM, IgA, and IgE antibodies in infectious mononucleosis.

Fifty infectious mononucleosis (IM) and 150 non-infectious mononucleosis (non-IM) sera were tested by a hemadsorption immunocapture test (HIT) for the detection of heterophile antibodies of IgM, IgA, and IgE classes. The specificity of Paul-Bunnell (PB) antibodies was ascertained by a differential absorption test. IgM PB-antibodies were demonstrated in 100% of IM sera, IgA in 92%, and IgE in 88%. PB antibodies were present only in IM sera; but other heterophile antibodies of IgM (68%), IgA (6.7%), and IgE (9.3%) classes were demonstrated in non-IM sera. IgA and IgE heterophile antibodies were thus present, especially in IM sera. HIT was found to be more sensitive than Paul-Bunnell Davidsohn test (PBD test) and suitable for the diagnosis of infectious mononucleosis.

Antibodies, Heterophile↗
PubMed1988

Microparticle enzyme immunoassay for determination of immunoglobulin G antibodies to human cytomegalovirus.

A microparticle enzyme immunoassay (LEIA) that uses passive latex agglutination was used to detect cytomegalovirus immunoglobulin G antibodies in 495 serum samples. LEIA was in excellent concordance with latex agglutination (97%) and an enzyme-linked immunosorbent assay (98.3%). The sensitivity and specificity of LEIA compared with those of the enzyme-linked immunosorbent assay were 100 and 92.5%, respectively.

Antibodies, Viral↗
PubMed1987

Solid phase reverse passive hemadsorption test for hepatitis B surface antigen.

A solid phase reverse passive hemadsorption test (SP-RPHAd) for hepatitis B surface antigen detection is described. It was compared with a commercial reverse passive hemagglutination assay (Hepatest, Wellcome, U.K.). SP-RPHAd is four-fold less expensive than Hepatest and undiluted sera can be used instead of eight-fold diluted sera without risk of non-specific hemagglutination. Thus, the threshold of detection is lowered to about 5 ng/ml by SP-RPHAd.

Enzyme-Linked Immunosorbent Assay↗
PubMed1987

[Response to influenza vaccine in uremic patients: relation to erythrocyte magnesium and the value of a second injection].

Humoral response to influenza vaccination being variable in uremic patients and being negatively correlated to red blood cell magnesium (RBC Mg) in non-uremic subjects, RBC Mg as well as plasma concentration of Mg were measured simultaneously with the antibodies titers after 1 and 2 injections of influenza vaccine in 21 non-uremic subjects and 47 patients on chronic hemodialysis. The RBC Mg and plasma Mg (MgP) were significantly higher in the dialysed patients (74 +/- 12 mg/l; 25 +/- 8 mg/l) than in the non-uremic subjects (54 +/- 5 mg/l; 19.4 +/- 1.5 mg/l). Furthermore, in the uremics RBC Mg was correlated to PMg whereas such a correlation was absent in the non-uremic patients. The humoral response of the uremic patients is depressed and becomes comparable to that of the non-uremic subjects after 1 injection only after 2 injections. In the uremic patients, the depressed humoral response is associated with higher RBC Mg (greater than 70 mg/l). The humoral response to influenza vaccine is depressed in uremic patients who have either the HBs antigen or no response to the hepatitis B vaccine but no link has been found between this immune status against the hepatitis B and RBC Mg. In conclusion, the humoral response of the uremic patients to influenza vaccine is depressed so that a second injection is necessary to give them sufficient protection.

Adult↗
PubMedDate not supplied

Amplification and detection of the terminal 3' non-coding region of hepatitis C virus isolates.

A reverse transcription polymerase chain reaction (RT-PCR) assay was set up to amplify, from chronically infected patients, the recently discovered hepatitis C virus (HCV) 3'non-coding region (3'NCR). A panel of 149 samples was tested by RT-PCR for the 3'NCR. Two detection methods of amplified products were evaluated: ethidium bromide staining on 3% agarose gel electrophoresis and DNA enzyme immunoassay ("DEIA"). Results were compared with those obtained by amplification of the 5' non-coding region (5'NCR), i.e. the "Amplicor" HCV RNA qualitative assay. Genotype distribution of the 86 Amplicor-positive samples was subtype 1a: n = 15 (17.4%); subtype 1b: n = 32 (37.2%); subtype 2a/2c: n = 7 (8.1%); type 3: n = 25 (29%); type 4: n = 2 (2.3%); type 5: n = 1 (1.2%); not determined: n = 4 (2.3%). Sixty-three sera were HCV RNA-Amplicor-negative, 32 of which were from HCV-seronegative patients and 31 from HCV-seropositive patients. All seronegative samples were negative by both PCR methods. None of the Amplicor-negative samples from seropositive patients were positive by the 3'NCR assay. Forty-seven (54.7%) and 83 (96.5%) of the 86 Amplicor-HCV-RNA-positive samples were positive after ethidium bromide staining and by the 3'NCR assay using DEIA, respectively. The limit of detection by end-point dilution was lower with Amplicor. No difference between genotypes was detected for the 3'NCR RT-PCR, and a high degree of concordance was obtained between the Amplicor and the 3'NCR DEIA results (97.4%). Nevertheless, further studies are needed before the 3'NCR RT-PCR assay could be used instead of the 5'NCR RT-PCR for diagnostic purposes.

Base Sequence↗