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Biomedical subjects

G Dominok

Publications and source records attributed to G Dominok.

13 recordsLinked to original sources
PubMed1988

[The effect of fixation, dehydration and polymethacrylate imbedding on the results of immuno- and enzymehistochemical studies on lymphatic tissue and bone marrow].

The influence of formaldehyde fixation, dehydration and polymethacrylate embedding on the results of immunofluorescence staining of immunoglobulin and enzymehistochemical demonstration of hydrolases, especially acid phosphatase and alpha-naphthylacetate esterase in human tonsils and bone marrow were studied. The best results were achieved by using 2% formaldehyde and 5% sucrose in 0.02 M phosphate buffer (ph 7.4) for fixation and ethyleneglycol monobutylether for dehydration. The procedures were carried out at 4 degrees C. For tissue embedding we used the commercially available polymethacrylate Kalloplast R. The polymerisation was carried out at -20 degrees C for 18 hours. This method permits a good preservation of morphological details and the survival of antigenic determinants and enzyme activity. Trypsin, pepsin and alpha-chymotrypsin were used to "unmask" the antigenic determinants in methacrylate sections. Trypsin and alpha-chymotrypsin revealed comparable results, but because of better practicability we used alpha-chymotrypsin. Using the described fixation, dehydration and embedding procedures it was possible to demonstrate both intracellular immunoglobulins (gamma, alpha and mu heavy chains; kappa and lambda light chains) and surface-bound immunoglobulins (mu and delta heavy chains; kappa and lambda light chains). Comparable results were achieved in human bone marrow biopsies. The results of histochemical demonstration of both enzymes were better in bone marrow sections than in that of the tonsils. We think, that the presented method is suitable in the diagnosis of myelo- and lymphoproliferative disorders on both the bone marrow and lymphatic tissue.

Bone Marrow↗
PubMed1988

[Immuno- and enzymehistochemical studies of methacrylate-embedded biopsy material, especially iliac crest biopsies].

Immunohistochemical and histochemical methods are increasingly used and their application in surgical pathology is obvious. Especially we used these methods on bone marrow core biopsies. Optimal and comparable results have been obtained by using different methods after halving the biopsy cores longitudinally and/or transversally. The two halves were used for cytologic imprints. Two parts of the biopsy cores were embedded in polymethacrylate at low temperature (-20 degrees C). The methacrylate-embedded biopsy part for routine histology was fixed in Schaffer's solution (methanol-formalin-fixative). The methacrylate-embedded undecalcified section of 4 microns may be stained by most stains commonly employed in routine histopathology after removal of the plastic. The sections are virtually free of artefacts such as shrinkage and swelling in the light microscope. The second methacrylate-embedded part of biopsy cores was fixed in 2% paraformaldehyde with 5% sucrose in 0.02 M phosphate buffer (pH 7.4) and dehydrated in ethyleneglycolmonobutylether. All procedures were carried out at 4 degrees C. This method permits the use of immunohistochemical and histochemical procedures. The immunohistochemistry was carried out at sections of 4 microns after removal of the plastic with methoxide and use of proteolytic enzyme (0.1% alpha-chymotrypsin) to unmask antigens in sections. Surface and intracellular immunoglobulins were very well detected with the indirect FITC method. The histochemical procedures are carried out at sections of 7-8 microns after removal of plastic with xylene and toluol. The sections were incubated for specific esterase and nonspecific esterases, acid and alkaline phosphatase and then examined by light microscopy. A third part of biopsy cores may be immediately frozen, and cryostat sections are stained and evaluated for rapid diagnosis and used for immunohistologic analysis with mono- and polyclonal antibodies (FITC method) and/or histochemical investigations. Imprints of biopsy cores are evaluated for cytological, cytochemical and/or immunocytological analysis with mono- and polyclonal antibodies (FITC method). The cryostat sections and the imprints are fixed for all methods with 2% paraformaldehyde and 5% sucrose in PBS (0.02 M, pH 7.4) at 4 degrees C for 30 minutes. The best diagnostic results were obtained in the myelo- and lymphoproliferative disorders using the combination of methods described here. Examples were demonstrated.

Biopsy↗
PubMed1986

[Quantitative pathomorphological findings in chronic glomerulonephritis at autopsy: characterization of the grade of renal insufficiency for correlation studies].

The investigation is based on 49 autopsies at which chronic glomerulonephritis (GN) was found. The glomerular findings were classified by the WHO classification of renal diseases. The ratio of kidney weight (g) to body height (cm) gave a constitutional kidney index which correlated with the degree of global and segmental (more than 50%) sclerosis of glomeruli in chronic GN (diffuse intracapillary type). The negative correlative coefficient from simple linear regression was rho = -0.606. Chronic GN was then further subdivided into four groups which mutually correlate: diffuse sclerosing GN (so-called end-stage kidney). Index: 0.27 g/cm; diffuse sclerosing GN (over 80% glomerular sclerosis). Index: 0.49 g/cm; diffuse proliferative and extensive sclerosing GN (more than 50% and less than 80% sclerosis) Index: 0.59 g/cm; diffuse proliferative and mild to moderate sclerosing GN (less than 50% sclerosing). Index: 0.93 g/cm. The indices of the diffuse extracapillary GN were high in comparison with diffuse intracapillary GN. The kidney index of diffuse extracapillary proliferative and extensive sclerosing GN with over 80% fibrous crescents was 0.78 g/cm. Index of diffuse extracapillary proliferative and mild to moderate sclerosing GN, mostly epithelial and fibro-epithelial, few fibrous crescents was 1.30 g/cm. The mean values of the indices for normal kidneys were (males) 0.78-0.81 g/cm and (females) 0.73-0.75 g/cm (age: 20-60 years). These results may be used for correlative investigations with morphometric parameters of renal osteopathy.

Adolescent↗
PubMed1984

[Chordoid sarcoma of the urinary bladder].

A case report of a chordoid sarcoma of the urinary bladder of an 81 years old man is presented. After the first description of the extraskeletal chordoid sarcoma in 1948, this is the first report of the tumor of the urinary bladder.

Aged↗
PubMed1972

[Scleromalacia].

Explore the source record for details and available documents.

Aged↗
PubMedDate not supplied

[Uncharacteristic symptoms of the motor apparatus in gout].

Gout may present in such atypical forms as "primary-chronic", "masked", or "asymptomatic gout at all stages affecting almost any joint. These uncharacteristic presentations were found in 22 out of 124 patients observed. The disease of 4 patients is described as casuistics. Important conclusions of practical use for early diagnosis and early treatment are drawn. Every arthropathy in a male where the diagnosis is not certain, must be suspected as being due to gout. The diagnosis can be assured by detecting urate deposits in the tissue. Determinations of the serum uric acid level can only be indicative since they are subject to a variety of influences. In early disease the x-ray appearances may be described as normal; in later disease as "degenerative" in the joints or in the vertebral column. In an acute relapse the colchicine-test is of high specifity, but its interpretation may be difficult in the primary chronic forms. The other clinical manifestations of the gout syndrome are of high value for the selection of patients in whom urate deposits in the tissue must be sought.

Adult↗