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Biomedical subjects

G Di Coscio

Publications and source records attributed to G Di Coscio.

24 records · Page 2Linked to original sources
PubMed1978

Bioactivity of male (sperm transmitted) mouse mammary tumor virus as influenced by donor, recipient and age at treatment.

The sperm collected from mammary tumor virus (MTV)-carrying mice (C3H, BALB/cfC3H, BALB/cfRIII and RIII) was separately tested for mammary tumor-inducing activity in (BALB/c x C3Hf)F1, (BALB/c x RIIIf) F1, and BALB/c female recipients by i.p., injection of 0.1 ml of the sperm at 1-2 weeks or at 3 months of age. A total of 551 recipents was observed, including control mice. The results may be summarized as follow: 1) mammary tumor incidence in experiments with or without histocompatibility between sperm donor and recipient is the same; 2) bioactivity is related to the type of MTV (C3H, RIII) and to the type of recipient, not to the sperm donor; 3) the activity of RIII MTV released in the sperm appears to be less influenced by the age of recipients than is that of C3H MTV; 4) BALB/c recipients are more susceptible to C3H than to RIII sperm-released MTV; 5) (BALB/c x RIIIf) F1 hybrids are resistant to sperm-released MTV, especially to C3H MTV infection, and show a 34% incidence of late spontneous lymphomas inherited by the RIIf male parent; 6) (BALB/c x C3Hf) F1 hybrids are susceptible to both C3H and RIII sperm-released MTV and show a 30% incidence of late spontaneous mammary tumors due to genetic transmission of MTV by the C3H male parent.

Age Factors↗
PubMedDate not supplied

HPV testing and Pap test: role for a combined approach in a non-screened population.

The aim of the present study was to test the polymerase chain reaction (PCR) as a tool to identify human papillomavirus (HPV) in routine cytological samples scraped from the uterine cervix. Moreover, attention has been focused on the correlation between HPV types and early intraepithelial lesions. The study involved 586 women who had undergone conventional Pap test. Analysis of HPV infection was performed by PCR and HPV typing by dot blot. In a group of 78 cases histologically diagnosed as high-grade squamous intraepithelial lesions (HSILs), the cytological diagnosis was correct in 92.3% and the HPV test was positive in 89.8% of cases; combined positivity at Pap and/or HPV tests raised this figure to 99.0%. In a group of 67 cases histologically diagnosed as low-grade squamous intraepithelial lesions (LSILs), the cytological diagnosis was correct in 73.1% and the PCR-based HPV test was positive in 64.2%; combined positivity at Pap and/or HPV tests raised this figure to 91.0%. This study confirms the limitations of screening programs based on Pap test only. Our results suggest, in fact, that adding the HPV test to primary screening could increase the yield of preinvasive cervical lesions.

Adult↗
PubMedDate not supplied

[Small nodules of adenomatous hyperplasia in the cirrhotic liver. Treatment with percutaneous alcoholization].

Small adenomatous hyperplastic nodules (AHNs) are now detected with increasing frequency in cirrhotic livers thanks to the widespread use of ultrasonography (US) in patients with chronic liver disease. The management of these lesions, which are commonly considered as likely to evolve into hepatocellular carcinomas (HCCs), is difficult: indeed, the surgical resection of a questionable neoplastic lesion does not seem advantageous, if surgical hazards are considered; on the contrary, the simple US/bioptic follow-up may be untimely in the detection of the malignant transformation. Percutaneous ethanol injection (PEI) under US guidance, already employed in the treatment of small HCCs, was recently proposed as a viable therapeutic alternative for AHNs. Our experience includes 16 AHNs submitted to PEI over a 2-year period. The size of the lesions ranged between 0.7 and 2.3 cm. Each nodule was treated on an outpatient basis with 6 weekly ethanol injections; the total amount of alcohol delivered to each lesion was 8-17 ml. No complications occurred. PEI induced volumetric reduction and/or structural modifications of the lesions; 10 of them were hardly differentiable from the surrounding parenchyma at the end of treatment. One month after PEI, CT allowed the detection of 13/16 AHNs; all of them resulted hypodense and showed no contrast enhancement. Repeated biopsies produced necrotic material in all cases. During the follow-up (10-33 months, mean 18.3) no recurrences were observed. At the moment, PEI seems to be the most appropriate therapeutic approach for small AHNs.

Aged↗