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Biomedical subjects

G Degiovanni

Publications and source records attributed to G Degiovanni.

33 records · Page 2Linked to original sources
PubMed1975

Ultrastructural study of the relationship between immune cytotoxic lymphoid cells and target cells in vitro.

Ultrastructural details of the relationship in vitro between sensitized T lymphoid cells and YAC target cells are described. Within a few hours very close connections develop between these two cells. Narrow junctions with parallel plasma membranes and local evaginations of the lymphoid cell into recesses of the target cell are specific aspects. Very fine threadlike material is found between the two plasma membranes at the narrowest zones. The lymphoid cells concerned have an intermediate structure between ordinary small lymphocytes and blast cells. They do not seem to be modified in the process while YAC target cells are severly damaged. Polysomes are dispersed; mitochondria and rough endoplasmic reticulum are affected locally or generally. Later, nucleoli and chromatin become dense and pycnosis occurs. The significance of these results is discussed.

Animals↗
PubMed1975

[In vitro comparison of 2 expressions of cellular immunity induced by a cutaneous allograft in the mouse].

A short-term test (5 h) has been compared to a long-term test (48 h) for evaluation of the cellular cytotoxic response which is induced in recipients of allografts. Spleen cells from mice recipients of skin allografts were tested. We have shown that the cellular cytotoxicity expressed in a short-term test is itself expressed during a short period whereas the cytotoxicity expressed in a long-term test is still detectable for months after a first skin graft. Moreover, the lytic activity of the spleen cells is much higher when it is expressed in a long-term test. The fact that we could get, in vitro, in two days, a low primary cellular cytotoxic response against alloantigens, makes likely that during the long-term test, the anamnestic response which can develop is especially responsible for the cellular cytotoxic response.

Animals↗
PubMed1975

[In vitro distinction between primary and secondary cellular response to alloantigens by means of a subcellular antigenic preparation].

Living spleen cells and a subcellular product of spleen cells have been compared, in mice, for their ability to elicit, for a long term culture (4-5 days), a cellular cytotoxic response against alloantigens. Contrary to living spleen cells which could induce the same high level of cytotoxic activity in a primary or in a secondary response, the antigenic preparation was only able to mount a very low primary response while it could render highly cytotoxic a lymphoid population proved to be able to mount in vitro an anamnestic response.

Animals↗
PubMed1975

[Trial of purification and serological characterization of human and murine transplantation antigens].

Human and murine antigens are purified by exclusion chromatography on Sephadex and preparative polyacrylamide gel electrophoresis and their purification stage checked by analytical methods. The study of antigenic preparations consists of electrofocusing, molecular weight estimation and chemical determinations. A cytotoxicity inhibition test with specific alloantisera reveals the antigenic potency. A microtest using 51Cr labelled tumoral cells has been achieved for the analysis of murine preparations.

Animals↗