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Biomedical subjects

G De Luca

Publications and source records attributed to G De Luca.

At least 199 records · Page 11Linked to original sources

Upper airway muscles dysfunction and levodopa responsiveness in Parkinson's disease.

Pulmonary function tests were performed before and at different times after 250 mg L-Dopa in 12 patients with Parkinson's disease (PD). Six were de-novo patients, the other six patients had been taking L-Dopa over different periods. All patients had an abnormal basal flow-volume loop, which significantly improved only in de-novo patients. This improvement occurred early and was independent on improvement of neurological symptoms. The effect of L-Dopa on pulmonary function could be a useful test in differentiating PD from related extrapyramidal syndromes.

Aged↗

Subcellular distribution of pyridoxal kinase in ox retina.

PL kinase activity has been determined in primary and secondary subcellular fractions of ox retina. Enzymic activity is predominantly located in the soluble fraction (S3). About 65% of the recovered PL kinase activity of crude mitochondria is released from synaptosomal fraction after hypoosmotic treatment. PL kinase activity in supernatant fraction (S3) does not exceed the enzyme activity measured in the homogenate, excluding the presence of a particulate-bound inhibitor to PL kinase in the homogenate. The possible physiological significance of PL kinase cellular compartmentation has been considered.

Animals↗

[Environmental diffusion of Legionella spp and legionellosis frequency among patients with pneumonia: preliminary results of a multicentric Italian survey].

A multicentric Italian investigation on legionnaires' disease is in course to clarify host factors as well as pathogen associated characteristics involved in the infection/disease. The main goal of the research plan is to account for some critical aspects concerning identification and prevention of legionellosis. To improve knowledge on factors associated with Legionella spp colonisation in hot waters, to detect cases and to characterize risk factors in subjects which develop pneumonia are specific objectives of the research programme. Preliminary results show that hot waters of houses and hotels are frequently contaminated (22.6% and 54.6%, respectively), mainly by L. pneumophila. Microbial concentrations were low in domestic waters (<1.000 ufc/l), but higher in samples from the hotels (geom. mean 1.85 x 10(3) ufc/l). Warming system, age of the plant, type of building were risk factors significantly associated with Legionella spp positivity. The active surveillance on patients affected by pneumonia with search for Legionella urinary antigen allowed the identification of 34 cases, 3 of which of nosocomial origin, corresponding to 4.2% of the screened pneumonia. After informed consent, 26 subjects were recruited for a case-control-study to clarify risk factors for the disease.

Humans↗

[Tobacco smoking among nursing students: behaviour and knowledge of the correlated risks].

This study was designed to assess knowledge and consumption of tobacco, during the three years of the university course for nurses in Bologna. A questionnaire consisting of items about personal details, smoking habits, and one open question about knowledge of health risks linked to tobacco consumption 505 students. 43% of males and 33% of females were current smokers. The males were mostly medium and heavy smokers (>11 cigarettes/day) and the females light smokers (<10/day). As regards knowledge an increase in both number and accuracy of the answers given by the third year students was seen. Suggestions for educational programs are given considering that the population in question is part of an "example group" who are supposed to play a role in influencing the behavior of the general population.

Adult↗

[Comparison of methodologies to reach 'dry weight'].

BACKGROUND: In clinical practice it is very difficult to determine a final weight that is the expression of normovolemia. In hemodialysis (HD), 'dry weight' is conventionally defined as the weight reached by the patient at the end of that hemodialytic session when the maximum quantity of fluids is removed without inducing any symptomatology. The determination of dry weight has been based on the application of clinical criteria. The use of artificial kidneys with blood volume (BV) sensors has allowed the determination of dry weight through the interpretation of changes in the intradialytic BV curve. Conventional bioimpedance analysis (BIA), or better, the vectorial BIA (BIVA) is a new method for determining dry weight. This study evaluated the use of the above-mentioned method for the proper governing of dry weight. PATIENTS AND METHODS: Twenty HD patients were observed for 4 weeks. In the 1st week, the clinical symptomatology of every patient was monitored during both HD sessions and interdialytic periods. During the 2nd week, intradialytic changes in the BV of each patient were observed on artificial kidneys. In the 3rd week, a cardiologist monitored patients before and after hemodialytic treatments. In the 4th week, the body composition of each patient was analyzed through bioelectrical bioimpedance. RESULTS: Patients, who had clinically shown symptoms of hyperhydration, to the contrary at BIA were dehydrated. Conversely, patients who had dehydration symptoms presented signs of hyperhydration at BIA. CONCLUSIONS: BIVA is the diagnostic instrument that more accurately demonstrates the hydration state of hemodialytic patients. It contributes in defining dry weight more efficiently.

Adult↗

[Physical growth of school-children in Trent. II. Anthropometric standards and auxological evaluation of pupils at the Elementary School].

The purpose of this research was to make an auxological study of the child population in Trento. The study was carried out in three stages: in the first stage standards were established for height and weight, i.e. "anthropometric models" to be used as a reference in the subsequent assessment of children from Trento using an auxological method; during the second stage the most suitable auxological method was selected and the levels of normality, paranormality and extranormality of the anthropomorphic features were calculated; in the third stage, the above-mentioned auxological method was used to assess the growth in height and weight of the individual subjects, taking into consideration the frequency and type of auxopathies in the Trento child population. As regards the first stage of the study, after measuring the height and weight of almost all the Trento children between the ages of 6-11, the average values were calculated and the relative standard deviations of height and weight at the different ages and the weight as compared to the different heights considered. The "experimental curves" obtained were then smoothed by calculating the equations of the technical development curves of the characters examined with the best fit procedure. The smoothed values are shown in Tables 2 and 4 and the corresponding curves are given in the graphs 1-6 (median curves with a dotted line). An examination of the data in Table 2 reveals that between 6 and 10 years, males surpass the females in height and in weight, whereas, starting from 10 years and 4 months, the mean height-weight values become greater in the females due to the earlier prepuberal-puberal auxological thrust which occurs in females. After the age of 13, as can be seen in similar auxological studies recently carried out by us in Trento adolescents aged 11-14 (Tonelli et al., 1988), when the auxological thrust also takes place in males, the height of the latter regains supremacy. The mean height and weight values measured by us in 1985 in Trento school-children aged between 6-11 was then compared with those found in their peers almost twenty years earlier (1966-67) by Bergamo et al. (1968), the increases in these features during the period considered was also calculated. This comparison revealed that during the aforementioned period there was a clear increase in somatic dimensions of Trento children at all the ages examined, which varied from approximately 37 cm for height to approximately 1.5-4 kg for weight.(ABSTRACT TRUNCATED AT 400 WORDS)

Adolescent↗

Biosynthesis of glycosaminoglycan precursors: evidences for different tissue specific forms of UDP-glucose dehydrogenase.

UDP-glucose dehydrogenase (UDPGDH) was extracted and partially purified from different rat tissues and the kinetic parameters and some properties of the enzyme were determined and compared. The pH optimum ranged between 8.6 and 9.4 for liver and kidney UDPGDH and between 8.4 and 8.6 for skin and lung UDPGDH. Liver and kidney enzymes showed a similar affinity for both UDPG and NAD. Lung and skin enzymes also showed similar affinity for both substrates, which differed however from that of liver and kidney UDPGDH. Both liver and kidney enzymes had a higher heat stability and a different electrophoretic mobility compared to skin and lung UDPGDH. These data suggest the existence of different tissue specific forms of the enzyme.

Animals↗

For an elective surgical management in lumbar intervertebral disc herniation. Experience of 25 years of surgery.

On the base of a wide clinical experiences, the Authors report some consideration about relationship between clinical findings and neuroradiological pictures (plain film, myelography, CT and NMR) of the lumbar vertebral and disc diseases. It's pointed out the useful aspect of each imaging technique that contribute to obtain the more complete informations about anatomy of the lesion; such considerations allows the surgeon to plain the more adequate surgical technique. The Authors think myelography, still today, is the investigation of choice in this kind of pathology.

Adult↗

[Carcinoid of Meckel's diverticulum (a case associated with adenocarcinoma of the rectum)].

The authors present a clinical case characterized by a rare combination of diseases: Meckel's diverticulum carcinoid and intestinal neoplasm. The clinical aspects and related implications are dealth with at some length, showing the relationship between these and tumour size as well as their importance in the therapeutic management and prognostic evaluation of the carcinoid tumour.

Adenocarcinoma↗

Regulatory mechanisms of UDP-glucuronic acid biosynthesis in cultured human skin fibroblasts.

Kinetic parameters and regulatory properties of UDPGDH extracted from cultured human skin fibroblasts were determined and compared with those of UDPGDH from cornea and epiphysial-plate cartilage. Fibroblast enzyme showed an affinity for UDPG 7 times higher than cartilage enzyme and 42 times higher than cornea enzyme. UDP-xylose acted as a co-operative allosteric inhibitor, but under the same experimental conditions fibroblast enzyme was significantly less inhibited. These results were in agreement with the different GAG production of the cells we studied. Fibroblast UDPGDH activity was regulated by the NAD/NADH ratio and it was also affected by modifications of extracellular matrix composition. A significant increase of UDPGDH affinity for UDPG was observed after the treatment of the monolayers with Chase ABC.

Cells, Cultured↗

Effects of a calcium-antagonist (flunarizine) on cancer cell movement and phagocytosis.

Flunarizine, a calcium-antagonist drug that binds to calmodulin, was found to inhibit the migration of both B16 melanoma cells and M5076 macrophage-like cancer cells. The migration movement, both under agarose and through a polycarbonate filter membrane, was impaired by the drug. In M5076 macrophage-like cells Fc-dependent as well as Fc-independent phagocytosis were also found significantly reduced by flunarizine. Results are discussed in relationship with the previously observed effects of the drug on the growth rate of B16 melanoma cells in vitro.

Animals↗