Biomedical subjects

G D Serov

Publications and source records attributed to G D Serov.

At least 19 recordsLinked to original sources

PubMed1998

[Study of antibiotic sensitivity of microorganisms by the method of diffusion in agar layers].

The proposed method for assessing microorganism sensitivity in solid and semisolid liquid nutrient media provides more accurate results and permits isolation of resistant forms of bacteria and studies of antibiotic interactions. Bacteriological study of latent growth zones of bacteria, although rather long, makes the results more reliable. The method can be used for assessing the microflora sensitivity in patients without resorting to isolation of pure cultures and in biotechnology for isolation of new antibiotics.

Agar↗

PubMed1995

New restriction endonucleases from thermophilic soil bacteria.

Using the most effective rapid method for the detection of restriction endonucleases (ENases) in microorganisms, 32 thermophilic producers have been isolated. All strains belong to the genus Bacillus. Thermostable isoschizomers of ENases, such as AvaI, BbvI, BbvII, BclI, BsaBI, BsiYI, BsrI, BstEII, BstNI, Cfr10I, ClaI, FspI, HaeIII, HpaII, Ksp632I and SfeI, were isolated.

Bacillus↗

PubMed1993

[New type II and IIs restriction endonucleases from thermophilic bacteria of the genus Bacillus].

Restriction endonucleases have been isolated from 26 strains of thermophilic strains of the Bacillus genus, their recognition sequences were determined, and for 15 of them cleavage sites identified. The enzymes proved to be isoschizomers of known endonucleases BstNI, EarI, HaeIII, HpaII, Cfr10I, BsiYI, BclI, BbvII, BbvI, BstEII, BsaBI, BsrI, FspI, ClaI, SfeI.

Bacillus↗

PubMed1992

[Restriction endonucleases: the detection of new producer strains and the isolation of enzyme preparations].

The method for analysis of microorganisms for the presence of the modification-restriction systems has been developed. The method has permitted to detect more than 10 new producing strains of restrictases including microorganisms of Rhizobium genus. Some of them are promising for practical use. It has been shown that using selection of clones the strain productivity can be increased. The purification process for the majority of restrictases has been proposed. Some physical and catalytic properties of new enzymes have been studied.

Bacteria↗

PubMed1989

[Type II SsrI restriction endonuclease from Staphylococcus saprophyticus].

The recognition sequence and cleavage site for restriction endonuclease SsrI have been determined, the latter being 5'-GTT decreases AAC-3'. The enzyme was isolated from Staphylococcus saprophyticus strain and may be used in DNA investigation instead of its isoshizomer HpaI.

Base Sequence↗

PubMed1986

[Study of the properties of different Haemophilus species for their classification].

On the basis of the data obtained in the study of the properties of different Haemophilus species, their classification as a separate family is proposed. In solving the problem of the systematization of Haemophilus, their ecology and, aside from the known properties of these organisms, their sensitivity to sulfonol and antibiotics have been considered.

Anti-Bacterial Agents↗

PubMed1986