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G Cuny

Publications and source records attributed to G Cuny.

At least 91 records · Page 5Linked to original sources
PubMed1987

The relationships between the 5' end repeats and the largest members of the L1 interspersed repeated family in the mouse genome.

Analysis of a few large L1 elements has revealed two types of tandem repeats at the 5' end: A and F. In this study, the relationships between these repeats and a series of large L1 elements has been analysed. Most of cloned L1 repeats were shown to lack either A or F sequences at their 5' ends. F sequences are found less frequently associated than A sequences to the 5' ends of L1 and an evolutionary comparison shows that the A type was introduced more recently during the evolution of the mouse genome than the F type.

Animals↗
PubMed1987

Nucleotide sequence of the chicken proto-oncogene c-erbA corresponding to domain 1 of v-erbA.

The nucleotide sequence of the chicken proto-oncogene c-erbA, the cellular counterpart of the viral oncogene v-erbA domain 1, has been determined. The c-erbA gene has an exon-intron structure characteristic of the eukaryotic split genes. The c-erbA domain 1 is composed of at least eight exons and seven introns. Analysis of the sequence data reveals a long open reading frame of 239 amino acid residues that share highly significant homology with the viral protein. Our results show that the viral erbA oncogene is a truncated form of its cellular homologue. In fact the cellular gene is 42 nucleotides longer at its 5' coding extremity. The presence of a gag-specific (17/20) nucleotide stretch in this region favours the hypothesis of a homologous recombination event between the cellular erbA and the gag gene of a parental retrovirus. We have also sequenced 1400 bp of DNA lying 5' to the long open reading frame in search of the transcription initiation sites for the c-erbA messenger RNAs. Our findings and interpretations are presented here.

Animals↗
PubMed1985

The mosaic genome of warm-blooded vertebrates.

Most of the nuclear genome of warm-blooded vertebrates is a mosaic of very long (much greater than 200 kilobases) DNA segments, the isochores; these isochores are fairly homogeneous in base composition and belong to a small number of major classes distinguished by differences in guanine-cytosine (GC) content. The families of DNA molecules derived from such classes can be separated and used to study the genome distribution of any sequence which can be probed. This approach has revealed (i) that the distribution of genes, integrated viral sequences, and interspersed repeats is highly nonuniform in the genome, and (ii) that the base composition and ratio of CpG to GpC in both coding and noncoding sequences, as well as codon usage, mainly depend on the GC content of the isochores harboring the sequences. The compositional compartmentalization of the genome of warm-blooded vertebrates is discussed with respect to its evolutionary origin, its causes, and its effects on chromosome structure and function.

Animals↗
PubMed1984

Nucleotide sequence organization in the very small genome of a tetraodontid fish, Arothron diadematus.

We have investigated the sequence organization of the very small genome (DNA content/haploid cell c = 0.4-0.5 pg) of a tetraodontid fish, Arotron diadematus, by using two main experimental approaches. The first one, renaturation kinetics, showed that slowly reassociating, intermediate, fast and foldback sequences represented 87%, 7%, 5% and 1%, respectively, of A. diadematus DNA, which is, so far, the vertebrate DNA lowest in repeated sequences. The second approach, centrifugation in Cs2SO4/BAMD density gradients [BAMD = bis(acetatomercurimethyl)dioxane], showed that A. diadematus DNA can be resolved into several components, characterized by buoyant densities of 1.700, 1.704(5), 1.708, 1.702 and 1.723 g/cm3, and representing 15%, 73%, 4%, 4% and 2.5%, respectively, of total DNA. The last component comprised a satellite DNA and ribosomal DNA. A family of interspersed repeats, possibly related to the AluI family of warm-blooded vertebrates, showed an extremely specific genomic distribution, being present in only the 1.708 g/cm3 component, which it matched in base composition.

Animals↗
PubMed1984

[Aortic dissection and Horton's disease. 2 cases].

The authors report two cases of temporal arteritis complicated with dissecting aneurysm of the aorta. The first one is discovered on systematical physical examination, the second is revealed by a thoracic pain. Clinical course is favourable under steroid therapy in both cases. Eleven cases of such complication with pathological control are reported in literature. Physiopathological mechanisms are discussed and active inflammatory involvement of aortic wall seem to be the main cause.

Aged↗
PubMed1983

[The future of the aged patient at the end of an acute disease].

Elderly patients play an important role in the inpatient structure of departments of Internal Medicine. Although they pose many difficult problems during their admission due to multiple system disease, the problems often increase at the end of the therapeutic period when discharge from hospital is decided upon. Over half the cases are able to be sent home but the liaison with the domiciliary services is often poor, leading to reiterative hospital admission. A period in a medium-stay unit may provide a solution, but, due to insufficient numbers and uneven distribution, their function does not always meet the required demand: rehabilitation of the patient to his previous life style. Long-stay departments may be justified when there is a loss of autonomy and when regular medical care is needed. The exact place of these medicalised long-stay units within the hospital structure is still a matter of heated argument. What must be avoided is the creation of circuits of transfer of patients from one establishment to another. The present situation could be improved upon by the development of pre-hospital services, a better appreciation of the functional needs of the individual during hospitalisation, and a better liaison between the hospital and domiciliary services, by the creation of a range of options which do not favourise one or the other solution in too theoretical a manner.

Acute Disease↗
PubMed1982

Cytogenetical and biochemical characterization of a dG + dC-rich satellite DNA in the primate Cebus capucinus.

A very abundant and dG + dC rich DNA satellite from primate Cebus capucinus has been characterized in its cytogenetic and biochemical properties with the purpose of studying the correlation between the staining properties of heterochromatin and the base composition of the corresponding very repetitive DNA. The staining techniques, as well as incorporation of base analogues, show that the heterochromatin segments of C. capucinus chromosomes correspond to a dG + dC-rich satellite. This satellite was detected and isolated by centrifugation in density gradient, radioactively labelled and localized by in situ hybridization on heterochromatin segments.

Animals↗
PubMed1982

[Electrophysiologic study of Achilles areflexia of aged subjects].

The elderly often presents loss of Achilles tendon reflex. In this electrophysiological study the authors examined 19 patients with loss of the ankle jerk and with no known etiology of peripheral neuropathy. They compared them with 24 subjects who had no areflexia. The study included measurement of the H reflex, of the tendon reflex, of the conduction velocity of Ia fibers and of motor conduction velocity of the tibial nerve. Amplitude of H and tendon response and H/M ratio are diminished in the areflexic group. In 4 cases the H reflex is preserved while the tendon reflex is abolished. The meaning of these impairments is discussed.

Achilles Tendon↗
PubMed1982

Sequence organization and genomic distribution of the major family of interspersed repeats of mouse DNA.

We have investigated that organization and the distribution of a family of interspersed DNA repeats in the mouse genome. The repeats are at least 5600 base pairs (bp) in size and contain two contiguous BamHI endonuclease fragments, 4000 and 540 bp in size, the larger of which includes a 1350-bp EcoRI fragment studied by previous authors. The repeats are polymorphic in their restriction maps, and represent the major family of interspersed repeats in the mouse genome. The repeats are present almost exclusively in the two light major components of mouse DNA, and the base composition of their large BamHI fragments matches that of those components. The genomic distribution of the repeats is different from that of structural genes, which are present not only in the two light components but also in the two heavy components of mouse DNA. This distribution indicates that the repeats are not involved, at least in any simple way, in the regulation of gene expression.

Animals↗
PubMed1981

The major components of the mouse and human genomes. 1. Preparation, basic properties and compositional heterogeneity.

Main-band DNA from mammals and birds can be resolved by density gradient centrifugation techniques into three or four families of fragments of different dG + dC contents. These major DNA components are similar in their buoyant densities and relative amounts in all species tested and are observed in DNA preparations ranging in Mr from 2 X 10(6) to over 200 X 10(6). In the present work, the four major components of mouse and human DNAs were prepared and characterized in several basic properties: relative amounts, dG + dC contents, buoyant densities and compositional heterogeneity. The results obtained lead to the following conclusions: (a) the major DNA components of mouse and man form at least 85% and possibly the totality of the main bands of these DNAs; (b) they have very low compositional heterogeneities over a wide molecular weight range; (c) they derive from very large chromosomal DNA segments of fairly homogeneous base composition, for which the name 'isochores' is proposed. A comparison of the compositional heterogeneity of main-band DNAs from warm-blooded and cold-blooded vertebrates confirms our previous conclusion that these DNAs are characterized by a different sequence organization.

Animals↗
PubMed1980

An analysis of fish genomes by density gradient centrifugation.

DNA was prepared from 33 species of fishes representing 12 of the 31 orders of Teleostei and one order of Chondrichthyes. DNA samples were investigated to determine modal and mean buoyant densities in CsCl (Rho-0 and ), main-band asymmetry, intermolecular compositional heterogeneity and base composition. Modal buoyant densities ranged over 1.697-1.704 g/cm3, generally with identical or close values for DNAs from species belonging to the same order. Main bands were practically symmetrical in most cases; when present, asymmetries were on the heavy side of the band and rather modest. In most cases, compositional heterogeneities were equal to, or lower than, those of bacterial DNAs. Both the CsCl band symmetry and the low compositional heterogeneity strikingly distinguish fish DNAs from the DNAs of warm-blooded vertebrates and indicate a major discontinuity in genome organization in the vertebrates. The overall properties of the main bands of fish DNAs are very similar to those exhibited by the light components of DNAs from mammals and birds.

Animals↗