Search PubMed⌕ Search

Biomedical subjects

G Cseh

Publications and source records attributed to G Cseh.

At least 19 recordsLinked to original sources
PubMed1991

[Capsaicin-induced mouse ear edema and its management].

Oedema was induced in one ear of male mice of the CFLP strain with capsaicin solution (10 microliters/40 micrograms/ear). The development in time and the extent of the oedema were determined by the oedema-disk gravimetric technique. The maximum oedema was attained after 1 hour, and there was subsequently a gradual decrease. The extent of the mouse ear oedema induced in this way and measured after 60 minutes was inhibited to a statistically significant degree and in a dose-dependent manner by the antihistamine chloropyramine, the antihistamine-antiserotonin cyproheptadine, the non-steroidal antiinflammatory agent pyroxicam, the prostaglandin antagonist di-4-phloretin phosphate, and the lipoxygenase inhibitor nordihydroguaiaretic acid. The method proved suitable for the detection and biologically quantitative determination of the state of desensitization induced with capsaicin.

Animals↗
PubMed1986

Sensitive method for the determination of 6-oxo-prostaglandin F1 alpha by gas chromatography with electron-capture and mass-fragmentographic detection.

A selective and sensitive quantitative method is described for the determination of the endogenous prostacyclin content of the aorta of rats, treated with GYKI-11679, a new hydrazone derivative with antihypertensive action. The thoracal aorta from three rats was excised and pooled, 1 h after treatment with 10 mg/kg of GY-KI-11679. The prostacyclin, transformed to 6-oxo-PGF1 alpha, was extracted. For gas chromatographic electron-capture detection (GC-ECD), 6-oxo-PGF1 alpha was converted into the O-methyloxime tris(trifluoroacetyl) hexafluoroisopropyl ester derivative. The GC-ECD properties, stability and mass-spectrometric characteristics of this new derivative allow the quantitative analysis of 6-oxo-PGF1 alpha by using nor-PGF2 alpha as internal standard.

6-Ketoprostaglandin F1 alpha↗
PubMed1985

Synthesis and biological properties of 16(S)-amino-PGF2 alpha methyl ester.

A synthesis of 16-amino-derivatives of PGF2 alpha is reported. Introduction of an amino group into position 16 of PGF2 alpha has decreased the sensitivity of the compound to metabolic degradation. 16(S)-amino-PGF2 alpha methyl ester shows high abortifacient activity with reduced diarrhoeic side effect.

Abortifacient Agents↗
PubMed1980

Prostaglandin profiles in nervous tissue and blood vessels of the brain of various animals.

The endogenous formation of prostaglandin (PG) D2, E2, F2 alpha, and 6-keto-PGF1 alpha was determined in homogenates of mouse, rat, and rabbit brain, and of rat cerebral blood vessels, using gas chromatography mass spectrometry. In all species tested, 6-keto-PGF1 alpha could be identified in the brain homogenates, but was a minor component in relation to other PGs. In contrast 6-keto-PGF1 alpha was the most abundant PG in the blood vessels, being present in about 40-fold higher levels than in the brain tissue. PGD2 was the most abundant PG in rat and mouse brains, but was below detection limits in the analyzed blood vessels. These studies indicating differential metabolism of PG endoperoxides in nervous and vascular tissue, provide a biochemical basis for further studies on the role of the PGs in brain circulation and neuronal activity.

6-Ketoprostaglandin F1 alpha↗
PubMed1979

A novel method for the purification of 15-hydroxy-prostaglandin-dehydrogenase from swine lungs.

15-Hydroxy-prostaglandin-dehydrogenase was purified 3 to 5 fold and with almost 100% yield from swine lungs by a novel method of precipitation with Rivanol after which it was further purified (8-10 fold) by gel-filtration. The optimal stability parameters of the purified enzyme preparation were determined and the behaviour of PGDH in polyacrylamide-gel-electrophoresis was studied by local enzyme detection. The enzyme seems to be composed of subunits. KM values of PGDH with respect to NAD, PGE2 and PGF2alpha were found to be 0.27, 0.017 and 0.038 mM, respectively.

Animals↗