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Biomedical subjects

G Crama-Bohbouth

Publications and source records attributed to G Crama-Bohbouth.

6 recordsLinked to original sources
PubMed1989

Are activity indices helpful in assessing active intestinal inflammation in Crohn's disease?

We have investigated the correlation of 24 h and 48 h faecal Indium-111 excretion with each other and with several clinical activity indices for Crohn's disease (CD): Crohn's disease activity index (CDAI), activity index (AI), simple index (SI), Oxford score, and laboratory parameters, such as ESR, serum albumin, orosomucoid, C-reactive protein, alpha-l-antitrypsin (alpha 1-AT) faecal concentration, and alpha 1-AT clearance in 58 CD patients (37 with small bowel and 21 with colonic disease). A significant correlation was found between 24 and 48 h faecal Indium-111 excretion for small bowel (r = 0.708, p less than 0.0001) and colonic disease (r = 0.994, p less than 0.0001). The median faecal Indium-111 excretion for colonic involvement (4%; 0.15-50% median and range) was significantly (p less than 0.005) higher than that for small bowel disease (0.45%; 0.03-2.9%). No significant correlation was found between faecal Indium-111 excretion and any activity index in the patients with small bowel disease, while in the group of patients with colonic localisation only the AI showed a significant correlation (r = 0.593, p less than 0.02). Faecal Indium-111 excretion was significantly correlated with alpha 1-AT clearance (r = 0.712, p less than 0.0001) and faecal alpha 1-AT concentration (r = 0.750, p less than 0.0001) in small bowel and in colonic localisation (r = 0.530, p less than 0.02 and r = 0.444, p less than 0.05). Serum albumin was significantly correlated only in the group of patients with colonic disease (r = -0.593, p less than 0.05). The present study shows poor agreement between activity indices, serum parameters of activity and faecal Indium-111 excretion. As a good correlation was found with the alpha1-clearance, which reflects losses into the gut, these results may suggest that faecal Indium excretion does not only reflect activity of inflammation, but my relate to the extent of intestinal ulceration.

Adolescent↗
PubMed1989

Immunological findings in whole and parotid saliva of patients with ulcerative colitis and healthy controls.

Since abnormal concentrations of immunoglobulins have previously been found in saliva of patients with Crohn's disease, we measured the concentrations of IgA, IgM, and IgG in whole and parotid saliva with the ELISA technique in 20 patients with ulcerative colitis (UC) and 19 healthy control subjects. IgA predominated in whole and parotid saliva in both patients and controls, and in both groups whole saliva had higher levels of IgA, IgM, and IgG than did parotid saliva. IgA and IgG levels were significantly (p less than 0.05) elevated in the whole saliva of UC patients as compared with the controls. IgM was also increased, but the difference did not reach statistical significance. No correlation was found between immunoglobulin levels and age, sex, duration of the disease, and extent of colitis. A significant negative correlation was found between the activity of the disease and the concentration of IgA in whole saliva. The present study therefore suggests that the previously reported abnormalities in the saliva of patients with Crohn's disease and presented as possible evidence for a disease affecting the whole of the gastrointestinal tract, are not specific, since similar findings are observed in UC.

Adult↗
PubMed1988

Plasminogen activators in the intestine of patients with inflammatory bowel disease.

Plasminogen activators were determined in intestinal tissue, obtained after surgery from patients with Crohn's disease and ulcerative colitis, and compared with normal intestinal tissue from colorectal cancer patients. The activity and quantity of tissue-plasminogen activator (t-PA) was found to decrease with the severity of inflammation in the patients with inflammatory bowel disease. Urokinase (u-PA) activity, however, was not changed compared with controls or in relation with severity of inflammation. In contrast, the level of u-PA antigen was found to be increased significantly in the inflammatory bowel disease tissues and was also related with severity of inflammation. The difference between u-PA activity and antigen in inflammatory bowel disease tissue could be attributed to an increase in inactive pro-u-PA and u-PA-inhibitor complexes. This increase in u-PA and the concomitant decrease in t-PA, are similar to those found in premalignant colonic adenomas, and might be related to the known increased cancer risk in inflammatory bowel disease.

Adolescent↗
PubMed1984

Immunological findings in whole and parotid saliva of patients with Crohn's disease and healthy controls.

Since oral lesions appear to occur more commonly in patients with Crohn's disease (CD) than in normal controls or patients with ulcerative colitis, it has been suggested that a deficit of locally produced IgA allows the invasion of the oral mucous membrane by antigens and contributes to the production of oral lesions. In connection with this hypothesis, the concentrations of IgA, IgM, and IgG in whole and parotid saliva were investigated by the ELISA technique in 20 patients with CD and 20 healthy subjects matched for dental status. IgA predominated in whole and parotid saliva in both patients and controls, and in both groups whole saliva had higher levels of IgA, IgM, and IgG than parotid saliva did. Compared with the controls, IgA, IgM, and IgG levels were significantly elevated in the whole saliva of CD patients. However, no correlation was found between immunoglobulin levels and age, sex, therapy, duration, localization, or activity of the disease.

Adult↗
PubMed1983

Immunohistological findings in lip biopsy specimens from patients with Crohn's disease and healthy subjects.

Biopsies of apparently normal buccal mucosa were taken from 14 patients with Crohn's disease and 13 healthy controls who were matched for dental status. Most patients had an increased number of lymphocytes around vessels in the subepithelial tissue and two showed fibrosis with moderate atrophy of minor glands. Plasma cells which contained immunoglobulin, predominantly IgA, were only found around minor salivary glands in both patients and controls. Quantitative studies showed a significant increase in the number of cells containing IgA in patients compared with controls. No correlation was found between immunoglobulin pattern and disease activity, age, sex, or duration of Crohn's disease. A significant correlation was found between the activity of the disease, as defined by the Crohn's Disease Activity Index, and the number of plasma cells containing IgM.

Adult↗