Search PubMed⌕ Search

Biomedical subjects

G Costa

Publications and source records attributed to G Costa.

At least 217 records · Page 12Linked to original sources

Physiological cost of apple-farming activities.

Seventeen agricultural male workers, aged between 21 and 56 years, were investigated in the field during six different job-activities of apple-farming: pruning, weeding, hand and mechanical spraying, mowing and picking. Pulmonary ventilation and oxygen consumption were recorded for short periods by Oxylog, while heart rate was monitored for more than 24 hours by Holter's method. The farmers also rated the work intensity according to Borg's RPE scale. Pulmonary ventilation ranged on average between 13 and 30 1/min and oxygen consumption between 500 and 1300 ml/min, with a relative aerobic cost between 15 and 40%, in the different work activities. Heart rate varied on average between 80 and 94 beats/min with a relative cardiac cost between 20 and 30%. The physiological criteria (oxygen uptake and cardiac response) and the subjective rating of fatigue gave a substantially similar evaluation of the work strain, 'moderate-heavy' for mowing, weeding and picking, 'moderate' for pruning and hand spraying, 'light' for mechanical spraying of pesticides. On the whole, apple-farming can be evaluated as 'moderate' intensity, thanks to the mechanisation implemented in reducing the work-load and the work-time of many job activities.

Journal Article↗

Modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats.

The state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (Ki) for the circulating tracer [14C]alpha-aminoisobutyric acid. Pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral glucose utilization; on the contrary, when rats were anaesthetized with ketamine, cerebral glucose utilization increased in the striatum and hippocampus and decreased in the cerebellum and brain-stem. It is suggested, as a hypothesis, that two different mechanisms, depending on the kind of the anaesthetic drug used, may be involved in the changes in the permeability of the blood-brain barrier, observed in anaesthetized animals: (a) a neurogenic component; (b) a direct interaction of the anaesthetic with elements of the microvasculature.

Aminoisobutyric Acids↗

Circadian characteristics influencing interindividual differences in tolerance and adjustment to shiftwork.

The study was carried out to evaluate whether shiftworkers showing different long-term tolerance to shiftwork differ in their circadian adjustments and/or in some behavioural characteristics. Three groups of eight workers, engaged on three shifts in a graphic plant and matched for age and work experience, were selected according to the presence or not of complaints related to shiftwork: (1) no complaints; (2) nervous complaints (anxiety/depression, severe sleep disturbances); (3) digestive disorders (gastroduodenitis, peptic ulcer). They answered questionnaires on family conditions, health status, rigidity of sleeping habits, ability to overcome drowsiness, morningness, manifest anxiety. They also recorded several physiological parameters (oral temperature, grip strength, peak expiratory flow rate, pulse rate, sleep hours) during day and night-shifts. The data obtained indicate that the characteristics of flexibility of sleeping habits, ability to overcome drowsiness, and lower manifest anxiety, are associated with better tolerance to shiftwork. These characteristics do not seem to influence the adjustment of the circadian rhythm of oral temperature passing from day to night-shifts and vice versa. Conversely, morningness appeared to be unrelated to long-term tolerance, but did influence circadian adjustments and sleep behaviour. Among the groups, the subjects with digestive disorders showed a greater phase shift and a reduction of the amplitude on night-work, suggesting a possible relationship also between the short-term circadian adjustment and the long-term tolerance to shiftwork, as pointed out by other authors.

Adaptation, Psychological↗

Treatment of advanced and/or metastatic epidermoid carcinoma of the head and neck: an effective combination chemotherapy with bleomycin, methotrexate and ftorafur.

29 evaluable patients with advanced and/or metastatic epidermoid carcinomas of the head and neck were treated with a combination of bleomycin, methotrexate and ftorafur. 3 complete remission, 12 partial remission (objective response 51.7%), 10 stable disease and 4 progressed disease were obtained, with the best responses in the oral cavity and skin carcinomas. The grade of performance status and the pretreatments affected the response, with a very high response rate in nonpretreated patients, where objective results were obtained in all cases. The toxicity was very mild. We believe that this combination can be recommended for epidermoid carcinomas of the skin and oral cavity, considering its high response rate, feasibility and low toxicity.

Adult↗

A mortality cohort study in a north Italian aircraft factory.

Mortality in a cohort of 8626 workers employed between 1954 and 1981 in an aircraft manufacturing factory in northern Italy was studied. Total follow up was 132,042 person-years, with 76% accumulated in the age range 15 to 54. Median duration of follow up from the date of first employment was 16 years. Vital status was ascertained for 98.5% of the cohort. Standardised mortality ratios were calculated based on Italian national mortality rates. Altogether 685 deaths occurred (SMR = 85). There was a significant excess of mortality for melanoma (6 cases, SMR = 561). Six deaths certified as due to pleural tumours occurred. No significant excess of mortality was found in specific jobs or work areas.

Adolescent↗

Intrinsic spontaneous activity and beta-adrenoceptor-mediated tubal dilatation affect ovum transport in the oviduct of the cow.

Oviducts of cows were obtained during the proliferative and secretory phases of the oestrous cycle (determined by macroscopic appearance of the ovaries). Consistently higher frequencies of contraction were observed in the isthmus than in the ampulla, whereas no significant difference was observed between longitudinal and circular specimens, or throughout the oestrous cycle. Basal activity was inhibited by removal of extracellular Ca2+ and by verapamil (10(-5) M). Addition of Ca2+ restored activity in the first case, but not in the second. Administration of various adrenoceptor agonists and antagonists revealed that the responses of the longitudinal and circular smooth muscles were primarily mediated by beta-adrenoceptors (beta 2 greater than beta 1), while the alpha-adrenoceptor-mediated contractions (alpha 2-subtype) were masked by the marked beta-adrenergic dominance. The results support the concept that the significance of adrenergic nerves in the cow oviduct may be to produce relaxation rather than contraction; the combined beta 1- and beta 2-adrenoceptor-mediated tubal dilatation and the intrinsic spontaneous activity seem to be the main factors affecting ovum/embryo transport throughout the oviduct in cows.

Animals↗

Label distribution after injection of labelled tachykinins into the rat lateral cerebroventricle.

The present study investigated the label distribution in several brain regions, as well as in the peripheral circulation, following injection of labelled tachykinins [( 3H]-substance P, [3H]-eledoisin or [125I]-neurokinin A) into the lateral cerebroventricle of the rat. A widespread label distribution, extending as far as to the brainstem, was detected. Hypothalamus, striatum and hippocampus were the most labelled regions by the 3 labels; however the patterns of distribution of the 3 labelled tachykinins showed marked differences. Distribution in the brain was rapid, reaching a maximum usually within 2 min after injection and declining slightly afterwards. Large amounts of label (1/6-1/15 of the total amount injected) were detected in serum even at 2 min after injection and increased thereafter, reaching a maximum at 10-15 min.

Animals↗

[Mortality in relation to the type of economic activity and occupation in the Turinese longitudinal study].

Occupational surveillance systems, which examine mortality patterns by occupation or industry, may contribute to hypothesis generation and priority setting for occupational cancer research and control. In the City of Torino the Local Population and Family Register records and the Census records are matchable through a computerized record linkage procedure. Since the early 1980's we have incorporated demographic events, occurring among residents, into the Census data source, covering birth, death and migration. This paper deals with the occupational mortality, from 1981 to 1985, of 262, 314 men and 146, 125 women, aged 15-64, who held a job at the 1981 Census. Mortality from 31 selected causes of death, including 22 cancer sites, among individuals employed in specific occupations (64 categories) and economic activities (80 categories) was compared with the mortality experienced by the whole cohort of active people. The surveillance design does not take into account potential confounders such as cigarette smoking, alcohol and previous work history. Despite the short duration of follow-up, some occupation-cancer associations, consistently documented in others surveillance studies, have been detected in our study: lung cancer among motor vehicle drivers (SMR 143, 27 obs), metal molders (SMR 178, 8 obs), welders (SMR 241, 7 obs) and wood workers (SMR 218, 12 obs), leukemias and electrical workers (SMR 367, 6 obs).

Adolescent↗

A second genetic locus for autosomal dominant polycystic kidney disease.

Hitherto, mutations that lead to autosomal dominant adult-type polycystic kidney disease have been found to be linked to the alpha-globin genes on the short arm of chromosome 16. In an Italian family, absence of linkage between the disease mutation and alpha-globin indicates that the condition can be caused by mutations in a second gene. The clinical features of the disease in this Italian family are indistinguishable from those found in the "linked" families. The finding that there are two polycystic kidney disease genes means that linkage must be demonstrated independently in each family before predictive tests with DNA probes can be used reliably.

Adult↗

Commuting--a further stress factor for working people: evidence from the European Community. I. A review.

About 100 million workers in the European Community commute to and from work daily. A review of the available data shows that commuting has increased in recent decades as rising car ownership has produced a more dispersed pattern of residential and job locations. In general, average commuting durations are falling, although average commuting distances are increasing as a result of faster commuting modes. However, the number of very long duration journeys have also increased, albeit from a small level. Up to now most research has focused on transport and land use issues, while very few studies have dealt with its impact on health, safety and social life of the workers. The available data indicate commuting to be a stress factor not only because of transport modes, but also by its interference with living and working conditions: namely, reduction of time available for discretionary leisure activities and increased absenteeism at workplace. Long-term effects on health have not been adequately investigated. Well integrated policies and strategies concerning the different aspects of this problem (transport, health, work organization, residential planning) must be developed both at the local and international levels to facilitate adequate solutions for this stressful condition.

Employment↗

Commuting--a further stress factor for working people: evidence from the European Community. II. An empirical study.

This report summarizes the main results of research promoted by the European Foundation for the Improvement of Living and Working Conditions, concerning the impact of commuting on the health and safety of workers. An empirical study, carried out among 1167 industrial Italian workers, shows that "commuters" (workers whose journey from home to work usually does not take less than 45 min in each direction) experienced a more stressed life-style than did "non commuters" (whose journey does not take more than 20 min). Commuting appears for many workers to be a necessity which is imposed by external factors, such as the housing market and job opportunities. Commuting is shown to interfere with patterns of everyday life by restricting free-time and reducing sleeping time. A majority of commuters use public transport mainly because of cost. Public transport commuters have problems due to more changes between modes, idle waiting times and delays leading to late arrival at work. Inside transport modes, commuters suffered discomfort as a result of overcrowding, microclimatic conditions, noise and vibrations. Commuters also reported higher psychological stress scores, more health complaints, essentially of psychosomatic nature, and greater absenteeism from work due to sickness. Commuting, in addition to shiftwork, further increases sleep problems, psychosomatic complaints and difficulties with family and social life. Women commuters were at a greater disadvantage than men, having more family difficulties, more travelling complaints and higher absenteeism.

Adult↗

Correlation between plasma levels of ACTH and beta-endorphin in the first seven days of postnatal life.

Plasma adrenocorticotropic hormone (ACTH) and beta-endorphin (beta-EP) concentrations were measured by radioimmunoassay in 122 newborns, born vaginally after spontaneous labor between the 38th and the 41st week of pregnancy. Blood samples were taken from umbilical cord in 10 newborns and from antecubital vein in the remaining 112 newborns, divided into 8 groups aged from 12 h to 7 days. The mean (+/- SE) ACTH concentrations in cord plasma were 81.87 +/- 10.16 pg/ml and decreased significantly (p less than 0.01) at the 24th h of life (49.09 +/- 6.93 pg/ml). Afterwards mean ACTH plasma concentrations fluctuated around the latter values. The mean (+/- SE) B-EP concentrations in cord plasma were 21.96 +/- 3.12 pmol/l and decreased significantly at the 24th h of life (13.43 +/- 2.08 pmol/l; p less than 0.01). From the 24th h to the 7th day the mean plasma concentrations of beta-EP were not significantly different. ACTH and beta-EP plasma levels were positively correlated (p less than 0.001) at delivery and during the first seven days of life.

Adrenocorticotropic Hormone↗

High intensity light exposure increases blood-brain barrier transport in rats.

A quantitative technique utilizing 14C-alpha-aminoisobutyric acid as a tracer was used to study the blood-brain barrier permeability modifications induced by environmental stimuli, as the high intensity light exposure. The blood-to-brain transfer constant was significantly increased only in the occipital cortex following the treatment. The possibility that the blood-brain barrier is able to modify its local characteristics in response to cerebral activity changes was discussed.

Aminoisobutyric Acids↗

Vasopressin release induced by intracranial injection of eledoisin is mediated by central angiotensin II.

Pulse intracerebroventricular injection of eledoisin, but not of substance P, markedly increases plasma vasopressin levels in the rat. Intracerebroventricular pretreatment with sarcosine1, alanine8-angiotensin II, 1 microgram/rat, completely suppresses the effect of eledoisin, suggesting that it is mediated by angiotensin release and angiotensin II receptor activation. The vasopressin releasing effect of eledoisin is neither due to peripheral haemodynamic alterations, nor to activation of the peripheral renin-angiotensin system. It is apparently related to central angiotensin release in a specific neuronal pathway subserving vasopressin release. This effect is not secondary to inhibition by tachykinins of the brain mechanisms for angiotensin-induced drinking, but is probably expression of direct activation of specific tachykinin receptors controlling vasopressin release.

Angiotensin II↗

Influence of endothelium on the response to calcium agonists, calcium, potassium and noradrenaline in rat aorta.

We have studied the influence of endothelium on BAY K 8644, CGP 28392, potassium, noradrenaline and calcium induced responses in isolated thoracic aorta from rats. In aortic preparations with intact endothelium BAY K 8466 and CGP 28392 (10(-9)-10(-5) M) had a concentration dependent contractile effect, but BAY K 8644 was more effective in contracting the vessels than CGP 28932. Removal of endothelium increased the contractile response to both substances without affecting the difference in contractile effect between the two calcium agonists. In high concentrations (10(-4) M) BAY K 8644 and CGP 28392 had an equal relaxing effect on potassium precontracted preparations, which was unaffected by removal of endothelium. Removal of endothelium caused a shift to the left of the potassium and noradrenaline concentration response curves without any change in maximal contractile response. The contractile response to calcium in BAY K 8644 and potassium activated vessels was unaffected by removal of endothelium. We conclude that endothelium derived factor(s) depress the contractile response to calcium agonists, potassium, and noradrenaline but has no influence on the relaxant effect of BAY K 8644 and CGP 28392 in rat aorta. The influence of endothelium on the contractile effect on BAY K 8644 and potassium seems not to be due to a direct effect on calcium influx but rather to an influence on the membrane potential of the smooth muscle cells.

3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethy↗

Chromosomal assignments of human type I and type II cytokeratin genes to different chromosomes.

The chromosomal location of representative members of the type I and type II subfamilies of the cytokeratin multigene family was determined using specific cDNA probes in Southern blot hybridization with DNA from somatic cell hybrids. Our results show that the gene encoding human type II cytokeratin 4 resides on chromosome 12 and that encoding type I cytokeratin 15 is located on chromosome 17. The results indicate that cytokeratins are not concentrated in only one cluster. The possibility of the existence of separate type I and type II cytokeratin gene clusters is discussed.

Blotting, Southern↗