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Biomedical subjects

G Cook

Publications and source records attributed to G Cook.

At least 91 records · Page 5Linked to original sources
PubMed1985

Recovery of the murine mononuclear phagocytic system following chronic exposure to cadmium.

Consistent with our previously reported findings, chronic ingestion of cadmium chloride in drinking water by mice caused a decrease in the rate of circulation clearance of 51Cr-labeled sheep red blood cells (E) and IgG-coated E (E-IgG) due to a decrease in the localization of E and E-IgG in the liver. These decreases reached their nadirs after 15 weeks of cadmium ingestion and remained relatively constant for up to one year during continued ingestion of cadmium. Replacement of the drinking water containing cadmium with regular tap water resulted within 8 days in an improvement in the ability of the mice to clear E and E-IgG. Mice also had a decreased ability to develop delayed-type hypersensitivity reactions while being given cadmium; this abnormality also returned toward normal after withdrawal of cadmium. The return of these two responses toward control levels occurred while there was still a large organ burden of cadmium that was not measurably different from that at cessation of cadmium ingestion.

Administration, Oral↗
PubMed1984

Tumoricidal response following perfusion over immobilized protein A: identification of immunoglobulin oligomers in serum after perfusion and their partial characterization.

Previously, we showed that perfusion of plasma from hosts bearing breast adenocarcinoma over immobilized staphylococcal protein A resulted in objective tumor regressions. In the present study, sera perfused in vitro over immobilized staphylococcal protein A were analyzed by physicochemical and immunochemical methods to characterize newly formed products. Sera from normal and breast adenocarcinoma-bearing dogs showed increased levels of C1q-binding IgG after perfusion over a strain of staphylococcus that is protein A rich (Cowan I), but not protein A deficient (Woods 46). C1q binding levels were also increased in normal and tumor-bearing canine or human sera which were perfused over purified protein A immobilized in collodion charcoal (PACC), and this increase was localized in sucrose density gradient fractions ranging from 7S to 19S. Polyacrylamide gel electrophoresis analysis of the high-molecular-weight fraction in postperfusion canine sera, isolated by G-200 fractionation and immunoaffinity chromatography, showed predominantly heavy and light immunoglobulin chains of canine IgG. Furthermore, protein A was released from PACC after perfusion with serum or solutions containing IgG or albumin from humans, dogs, and chickens. After serum perfusion over PACC, protein A was identified in the effluent by additional studies as follows: (a) polyacrylamide gel electrophoresis analysis showed that eluted 125I-protein A comigrated with the protein A marker; (b) postperfusion C1q-binding complexes, isolated by gel filtration under dissociating conditions and affinity chromatography on IgG-Sepharose showed a single precipitin band with normal human (protein A reactive) but not chicken (protein A unreactive) serum. Protein A released from PACC which appeared in postperfusion sera was associated with immunoglobulins in macromolecular complexes since (a) eluted 125I-protein A was largely (NH4)2SO4 and polyethylene glycol precipitable, whereas free protein A was not, and it sedimented in sucrose density gradient fractions distributed beyond the 7S marker, compared to free protein A which localized below 7S; (b) radiolabeled protein A eluting from PACC after serum perfusion showed 8-fold greater binding to C1q-coated tubes compared to free protein A; and (c) increased C1q-binding IgG in postperfusion sucrose density gradient fractions corresponded to the appearance of protein A in parallel gradient fractions.(ABSTRACT TRUNCATED AT 400 WORDS)

Adenocarcinoma↗
PubMed1983

Bromo-DMA: the Australasian hallucinogen?

If the frequency of seizures by police of hallucinogens reflects the frequency with which various hallucinogens are ingested in Australasia, most toxic states resulting from hallucinogen abuse are due to Bromo-DMA and not to LSD as is commonly reported by the subjects. Two cases of intoxication with the new hallucinogen, Bromo-DMA, are reported. Both recovered within 24 hours following treatment with haloperidol.

DOM 2,5-Dimethoxy-4-Methylamphetamine↗
PubMed1982

Dietary factors in relation to the etiology of colorectal cancer.

A case control study of 542 cases of colorectal cancer, 542 neighborhood controls, and 535 hospital controls has been conducted in two areas of Canada. The consumption of 13 nutrients has been estimated individually for all study members using an extensive diet history questionnaire. Highly significant dose-response relationships are observed between saturated fat intake and increased risk of both colon and rectal cancer for both sexes. In contrast no increase in risk was observed for intake of any level of polyunsaturated fat. The data are thus supportive of the hypothesis that dietary saturated fats may be of etiologic significance in cancers of the large bowel.

Colonic Neoplasms↗
PubMed1981

Recognition of bladder tumors by excretory urography.

Bladder tumors initially may be diagnosed by excretory urography in patients undergoing the examination because of specific symptoms or unrelated complaints. Among seven strategies tested by six experienced readers, the combination of early, full-bladder, and postvoiding anteroposterior radiographs was most successful in identifying bladder tumors in a series including 20 cases of biopsy-proved bladder tumor and 19 cases of cystoscopically normal bladders. The postvoiding radiograph, when viewed alone, was the single most valuable projection, and resulted in the correct diagnosis of 14 cancers that were not appreciated on any other single radiograph for a given case. The addition of oblique projections to the other three views did not significantly increase diagnostic sensitivity.

Carcinoma, Transitional Cell↗
PubMed1980

Successful management of serious disopyramide poisoning.

A case of deliberate disopyramide overdosage is described. Circulatory collapse was treated by means of a large dose of isoprenaline, and charcoal haemoperfusion was used in an attempt to enhance the elimination of disopyramide. The suitability of this treatment regime is discussed in the light of findings from animal studies and the implications for the management of the disopyramide-poisoned patient are considered.

Adult↗
PubMed1979

Extracorporeal immunoadsorption: initial experience in human systemic lupus erythematosus.

Extracorporeal immunoadsorption and filtration was used in treatment of a 29-year-old woman with severe lupus nephritis. For the previous 35 days single-stranded DNA (ssDNA) antibody and immune-complex levels had been continuously raised with only partial improvement on prednisone therapy. Levels of immune complexes and ssDNA antibodies were substantially reduced by extracorporeal plasma filtration and adsorption of DNA collodion charcoal. There were no major clinical complications. After perfusion, the reduction of ssDNA binding and immune complexes in serum was sustained, serum C'3 became normal, and serum creatinine and proteinuria improved. 28 days after perfusion, subendothelial glomerular deposits were much reduced compared with those in a pre-perfusion biopsy specimen. Selective immunoadsorption is a promising new approach to persistent lupus nephritis refractory to drug therapy alone.

Adult↗
PubMed1978

The Drug Attitudes Scale (DAS): its development and evaluation.

Five studies were conducted, employing three independent samples of high school students, leading to the development and assessment of the reliability and validity of a Drug Attitudes Scales. The results of these studies support the conclusions that this scale shows substantial reliability and validity, and promises considerable utility in drug treatment and education research. The Drug Attitudes Scale consists of 60 attitude items dealing with drugs and drug use, and is comprised of 10 six-item subscales referring to tranquilizers, barbiturates, heroin, opiates other than heroin, "speed," alcohol, cannabis, hallucinogens, tobacco, and general drug use.

Adolescent↗