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Biomedical subjects

G Cole

Publications and source records attributed to G Cole.

At least 91 records · Page 5Linked to original sources
PubMed1985

Histogenesis of haemangioblastomas: an immunocytochemical and ultrastructural study in a case of von Hippel-Lindau syndrome.

The cerebellar, retinal, and one of the spinal haemangioblastomas in a case of von Hippel-Lindau syndrome were studied by immunocytochemistry and electron microscopy. The tumours were positive for neurone specific enolase and variably positive for somatostatin, pancreatic polypeptide, and bombesin. Electron microscopy of the cerebellar tumour showed secretory granules with an average diameter of 170 nm. This report is believed to be the first description of neurone specific enolase positivity and polypeptide hormones within the intervascular cells of haemangioblastomas. In the light of these findings it is suggested that haemangioblastomas are tumours of neuroectodermal origin, derived either from neural or neuroendocrine cells.

Angiomatosis↗
PubMed1985

Alexithymia, hemisphericity, and conjugate lateral eye movements.

The conjugate lateral eye movements (CLEMs) of 102 university students were measured in conjunction with the administration of the Schalling-Sifneos Personality Scale, a self-report measure of alexithymia. There was a significant positive correlation between CLEM scores and alexithymia scores for all subjects, suggesting the existence of a relationship between right hemisphere activation and alexithymic characteristics. These results are discussed in terms of some theoretical models of alexithymia.

Adult↗
PubMed1985

Paraffinomas of the scalp.

Four cases of scalp paraffinoma were seen in our clinics within the past year. This unusual condition results from intradermal injection of substances containing paraffin. The presence of paraffin in excised tissue was confirmed by thin-layer chromatography and infrared absorption spectrophotometry. Our patients received their treatments 35 to 42 years ago after responding to radio or newspaper advertisements that promised a cure for baldness. Two patients were initially observed with severe scalp inflammation and two were completely asymptomatic other than having lumpy scalps. Although these cases were readily diagnosed by history, eliminating other possibilities was greatly aided by use of xeroradiography, obviating the need for biopsy in asymptomatic cases.

Aged↗
PubMed1984

Regulation of major histocompatibility gene expression in teratocarcinoma 402AX cells.

Teratocarcinoma 402AX cells are induced to express major histocompatibility complex (MHC) antigens when passaged in vivo in genetically resistant host mice. Studies reported here demonstrate that MHC antigen induction is regulated in vivo in part by the synergistic action of Lyt 1 and Lyt 2 positive splenic T cells and in vitro by serum from tumor-primed resistant mice. Northern blots suggest that some teratocarcinoma 402AX MHC class I antigens may be post-transcriptionally regulated.

Animals↗
PubMed1984

Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.

The filamentous ascomycete Aspergillus awamori secretes large amounts of glucoamylase upon growth in medium containing starch, glucose, or a variety of hexose sugars and sugar polymers. We examined the mechanism of this carbon source-dependent regulation of glucoamylase accumulation and found a several hundredfold increase in glucoamylase mRNA in cells grown on an inducing substrate, starch, relative to cells grown on a noninducing substrate, xylose. We postulate that induction of glucoamylase synthesis is regulated transcriptionally. Comparing total mRNA from cells grown on starch and xylose, we were able to identify an inducible 2.3-kilobase mRNA-encoding glucoamylase. The glucoamylase mRNA was purified and used to identify a molecularly cloned 3.4-kilobase EcoRI fragment containing the A. awamori glucoamylase gene. Comparison of the nucleotide sequence of the 3.4-kilobase EcoRI fragment with that of the glucoamylase I mRNA (as determined from molecularly cloned cDNA) revealed the existence of four intervening sequences within the glucoamylase gene. The 5' end of the glucoamylase mRNA was mapped to several locations within a region -52 to -73 nucleotides from the translational start. Sequence and structural features of the glucoamylase gene of the filamentous ascomycete A. awamori were examined and compared with those reported in genes of other eucaryotes.

Aspergillus↗
PubMed1984

Disc oedema in association with Hunter's syndrome: ocular histopathological findings.

The histopathology of the posterior half of one eye and the optic nerves of a man with Hunter's syndrome, who was known to have disc oedema without raised intracranial pressure for 11 years of his life, is reported. The possible pathogenesis of the disc oedema is discussed; an important contributory factor was the deposition of abnormal mucopolysaccharides within the sclera. This caused gross thickening of the sclera with compression of the optic nerve at the intrascleral level.

Adult↗
PubMed1984

Von Hippel-Lindau syndrome with microscopic hemangioblastomas of the spinal nerve roots. Case report.

The authors describe a case of von Hippel-Lindau syndrome diagnosed at autopsy in a 49-year-old woman. She died suddenly following hemorrhage from a cerebellar hemangioblastoma. Other autopsy findings included a retinal hemangioblastoma, an adrenal pheochromocytoma, and a clear-cell renal tumor. The case was distinguished by the unexpected finding of multiple microscopic hemangioblastomas of the spinal nerve roots. The case is discussed with emphasis on the incidence of spinal hemangioblastomas in von Hippel-Lindau syndrome.

Adenoma↗
PubMed1984

Histopathology of the spinal cord after intrathecal cocaine, bupivacaine, lignocaine and adrenaline in the rat.

Repeated intrathecal injections were given through catheters which had been chronically implanted in the subarachnoid space of rats. Injections were made of cocaine 0.25%, 0.125% and 0.0625%; bupivacaine 0.5%; lignocaine 2% and 0.5%; adrenaline 0.01% (1 in 10 000) and 0.002% (1 in 200 000); and sodium chloride 0.9%. Five injections of 35 microliter of each concentration of each drug were given hourly each day for two days to three rats. No clinical nerve damage was detectable in any rat. The only pathological change found in any rat was the development of cytoplasmic vacuolation in the neurones of the anterior and posterior horns. The changes were most prominent after injection of 0.9% sodium chloride and bupivacaine, whilst lignocaine (0.5 and 2%) showed the least number of vacuoles, which were also the smallest in size. Adrenaline and cocaine were intermediate in effect. There was no evidence of cell death. These changes were not seen in control animals in which catheters had been implanted but no injections had been given. The changes were mild and it is concluded that the agents tested caused no significant damage.

Animals↗
PubMed1983

Needle muscle biopsy in infants and children.

Percutaneous muscle biopsies were performed in 77 infants and children using the Bergstrom muscle biopsy needle and local anesthesia. Satisfactory specimens for histochemistry and electron microscopy were obtained in all but one case, and the procedure was well tolerated by all patients. No complications were observed. The percutaneous biopsy is a useful technique for the diagnosis of neuromuscular disorders in children.

Adolescent↗
PubMed1982

Size at birth of Asian and white Caucasian babies born in Leicester: implications for obstetric and paediatric practices.

This investigation extends our knowledge of the size of British born Asian babies compared with those of white Caucasian origin. Seventy Leicester born term Asian babies at birth were lighter, shorter, leaner and had smaller heads than their white Caucasian counterparts. The Asian mothers were shorter and lighter at the start of pregnancy but their weight/height2 index was the same. With the appreciation that the Asian population in Britain is far from homogenous in regard to religion, diet and country of origin, it is concluded that the smaller size at birth of Asian babies is unlikely to be due to undernutrition of their mothers in pregnancy. Genetic factors, as indicated by the shorter stature of the mothers are more likely to be responsible. These findings have implications for obstetric and paediatric practices.

Adult↗
PubMed1982

Acute cerebral demyelination: clinical and pathological correlation with computed tomography.

A 30-year-old woman died eleven weeks after the onset of an acute illness during which she developed quadriplegia, dysphasia, incontinence, confusion, emotional lability and gaze palsies. The CT scan demonstrated large white matter low attenuation lesions with no mass effect and minimal contrast enhancement. At necropsy the lesions were shown to be those of massive cerebral demyelination.

Adult↗
PubMed1981

Operational audit--the respiratory department.

Although our operational audit involved evaluating the cost effectiveness and efficiency with which procedures or functions are performed, our focus remained on the actual operational problem as defined in our criteria. The pre-determined standards, on which our criteria statements were based, provided the yardstick for measurement. Our assessment and Treatment Plan for patient care was audited, followed by a review of our policy and procedure compliance and the application of managerial systems and controls. Although the total impact of this audit tool on astute clinical care, management, judgement, and educational needs is yet to be addressed, we believe we have taken an important step in the direction of quality control through the initiation of an evaluation system in the Respiratory Department.

Concurrent Review↗
PubMed1979

Leucodystrophy of late onset in two members of a family.

We report 2 cases of demyelinating disease of unusually late onset in a mother and her son. The late onset of symptoms, at the age of 63 years in the mother's case, is rare. The onset in the son was at the age of 35 years. The relatively few adult cases previously reported range in age from 25 to 65 years, but only 2 cases in persons over the age of 60 could be found in the literature. The clinical and neuropathological findings are described.

Adult↗
PubMed1979

Crural hypertrophy associated with centronuclear myopathy.

A case of centronuclear myopathy of adult onset with striking hypertrophy of lower limb muscles in a black South African man is described with details of the light microscopic, histochemical, and ultrastructural features. The association of hypertrophied muscles with centronuclear myopathy has not to our knowledge been reported previously and it is felt that this may be a variant of the condition.

Adenosine Triphosphatases↗
PubMed1979

Centronuclear myopathy.

Centronuclear myopathy occurring sporadically in two African female children is reported, with details of clinical history and histological, histochemical, and ultrastructural findings, and a review of 58 previously reported cases. In spite of distinctive histological features, the clinical presentation of this condition is variable, there are different modes of inheritance, and the pathogenesis remains unclear.

Adolescent↗