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Biomedical subjects

G Ciprandi

Publications and source records attributed to G Ciprandi.

At least 127 records · Page 7Linked to original sources
PubMed1990

Levocabastine versus cromolyn sodium in the treatment of pollen-induced conjunctivitis.

Thirty patients with allergic conjunctivitis, caused by Parietaria or grass pollens, participated in a double-blind parallel study comparing levocabastine to cromolyn sodium, both given as eye drops. Symptom and sign scores were recorded during a 4-week period. The patients received only these drugs during the time of observation. The evaluation of the clinical signs and symptoms by the clinicians and by the patients revealed a significant improvement of conjunctivitis in all patients. The intergroup comparison was equal in the two groups treated respectively with levocabastine and cromolyn. Therefore, levocabastine and cromolyn are effective in the treatment of pollen-induced allergic conjunctivitis.

Administration, Topical↗
PubMed1989

Effects of tetroxoprim and sulfadiazine on T lymphocyte proliferation and gamma-interferon production.

The in vitro effects exerted by tetroxoprim and sulfadiazine on T lymphocyte proliferation were evaluated at drug concentrations equal to and twice the plasmatic peak level of each drug. The two drugs did not exert any significant effect on spontaneous and mitogen (PHA) or monoclonal antibody (MAb anti-CD3) induced T lymphocyte proliferation at the studied concentrations, both when tested separately and combined. In addition the two compounds did not significantly interfere in gamma-interferon (IFN-gamma) production.

Adult↗
PubMed1989

Antiallergic drugs and the immune response. Interactions and possible clinical relevance.

Since the pharmacological treatment of allergic diseases is used in patients with alteration of the immune response due to atopy and possible concomitant infections, we have investigated the possible effects of such drugs as cromolyn, theophylline, ketotifen, oxatomide, astemizole, fenoterol, pirenzepine, and rosaprostol on the in vitro immune response, in order to obtain experimental data and, as a consequence, clinical conclusions. In a series of investigations by our group and other authors the following immunological parameters have been considered: T cell activation induced by different pathways (i.e. autologous stimulation, PHA, anti-CD3, -CD2 and -CD28 monoclonal antibodies), and lymphokine production (i.e. IL-1, IL-2 and gamma-IFN). For a more detailed experimental model the experiments have been performed both in bulk culture and by using T cell clones derived from the peripheral blood. The results show cromolyn to have an enhancing effect, theophylline and ketotifen a suppressing effect, whereas the remainder show no effect on the immune response. These data are considered and discussed from the aspect of their possible clinical relevance and also in light of the in vivo data previously reported.

Antibody Formation↗
PubMed1989

[Treatment of large laparoceles with non-reabsorbable prosthetic material (our experience)].

A series of 96 patients who underwent "eventration repair" using Mersilene-Mesh, according to Rives technique between jan 1983 and june 1988 is reported. The Authors stress the excellent results and the low cost of this method: there were no recurrences, postoperative complications were rare (18.7%) and their recover was complete, the patient was allowed to return to work earlier after easy and prompt convalescence.

Adult↗
PubMed1989

Immunologic and clinical evaluation of a 12-month course of specific immunotherapy.

Allergen specific immunoglobulins (IgE, IgG, IgG1, and IgG4) were evaluated during the course of a 12-month specific immunotherapy (ITS) in 27 allergic subjects: 12 Dermatophagoides pteronyssinus (DP)-, 9 Parietaria officinalis (W19)-, six perennial and timothy rye grass (G5 and G6)-sensitive cases. Further, the modifications of the specific Ig levels were compared with the significant improvement in symptoms and drug consumption observed after 12 months of ITS. IgE levels significantly decreased after 6 and 12 months, while IgG1 significantly increased in the early phases of ITS (3rd month), and IgG4 significantly and progressively increased during the course of ITS. No modification was observed in specific total IgG. Regression analysis test among the various Ig levels revealed a strict correlation only between IgE and IgG4 (high levels of IgE before ITS correlated with high levels of IgG4 after 12 months of ITS). No correlation was observed between total Ig changes and improvement of clinical status or drug consumption.

Adolescent↗
PubMed1988

T cell activation through different membrane structures (T3/Ti, T11, T44) and frequency analysis of proliferating and interleukin-2 producer T lymphocyte precursors in aged individuals.

It is well known that the proliferative responsiveness of T cells of aged subjects is depressed in both autologous mixed lymphocyte reaction (AMLR) and in PHA-induced cultures. In the present study we analyzed T cell activation through different stimulatory pathways (such as T3/Ti antigen receptor, T11 complex and T44 molecule). Moreover, we studied Interleukin-2 (IL-2) release performing a limiting dilution analysis of the proliferative capability of peripheral blood T cells, employing a high efficiency cloning technique. Our results demonstrate normal proliferation of T3-induced T cells in aged subjects, whereas T11- and T44-induced T cell proliferations are depressed in aged subjects. In addition, studies at clonal level reveal a normal percentage of IL-2 producer T cell in aged individuals. In conclusion, our data suggest that the T cell in aged subjects are normal in number, but they have a decreased capacity of lymphokine production.

Adult↗
PubMed1988

Theophylline and the immune response: in vitro and in vivo effects.

We analyzed the in vitro and in vivo effects of theophylline on various immunological parameters including proliferation of peripheral mononuclear cells (PMNC) in response to phytohemagglutinin (PHA), anti-T3 and anti-T11 monoclonal antibodies (MAb), PHA-induced interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production by PMNC, interleukin-1 (IL-1) production by accessory cells, PHA-induced IL-2 production by T-cell clones, and PHA- and anti-T3 MAb-induced DNA synthesis by T cell clones. Results showed that theophylline inhibited PHA- and anti-T3-induced proliferation of both PMNC and T-cell clones, whereas the PMNC proliferation induced by MAb anti-T11 was not affected. The inhibition appeared to be dose-dependent and strictly related to the presence of the drug in the culture. Moreover, PHA-induced IL-2 production by both PMNC and T-cell clones also appeared to be reduced by theophylline. IL-1 production by accessory cells was not affected. These data suggest that the immunological inhibition exerted by theophylline is confined to the T-cell compartment, mainly by acting on structure(s) related to the T3/Ti complex, the primary site for T-cell activation. The alternative pathway of T-cell activation (i.e., via T11 site) seems unaffected. In addition, these results suggest possible clinical relations between the inhibition of the immune response and the plasma levels of the drug reached after a "once daily" or "twice daily" oral ingestion of slow-release theophylline products.

Adult↗
PubMed1988

Inhibiton of the colony-forming capacity of human T-lymphocytes exerted by theophylline.

The effects of theophylline on T-lymphocyte colony formation were investigated both by in vitro studies (adding theophylline in cultures at three different concentrations--5, 15 and 30 micrograms/ml) and by in vivo experiments (after oral ingestion of 10 mg/kg time-released theophylline) in a group of young healthy volunteers. In vitro the drug showed a significant dose-dependent inhibitory effect on both peripheral mononuclear cells (PMNC) and purified T-lymphocyte colony formation. No significant differences in PMNC colony formation before and 12 h after the oral ingestion of theophylline was detected in the in vivo study, even if at the same time the plasma concentration of the drug remained at therapeutic level in every subject. In addition, in vitro experiments mimicking the in vivo conditions suggested that the apparent disagreement between the in vitro and in vivo results was due to experimental procedures (i.e. the inhibitory effect requires the constant presence of the theophylline in the culture medium). The experimental data provided in this study confirm, using different experimental procedures, the results of our recent investigations showing that theophylline exerts an inhibitory effect on T-cell proliferation.

Adult↗
PubMed1988

Fenoterol effects on the in vitro immune response.

Beta-2-adrenergic agonists are often employed in the treatment of acute bronchostenosis. Following our recent investigations into the influence of some drugs (cromolyn, ketotifen, theophylline) on the immune response, in this study we analyzed the in vitro effects of fenoterol (beta-2-adrenergic agonist) on the immune response. The mitogen-(PHA)-induced proliferation of peripheral mononuclear cells (PMNC), the PMNC proliferation induced by anti-T3 and anti-T11 monoclonal antibodies (MAbs), the PHA-induced lymphokine--interleukin 2 (IL-2) and interferon-gamma (IFN-gamma)--production were studied in ten healthy volunteers. Since the plasmatic peak of fenoterol following a single inhalation of 200 micrograms is about 20 ng/ml, in the experiments herein reported the drug was tested in the cultures at concentrations lower, equal and higher than the plasmatic peak: respectively, 2, 20 and 200 ng/ml. Furthermore, for a more detailed study of T-lymphocyte activities, we also evaluated the effect of fenoterol on T-cell clone proliferation. Our results, which reveal no effects of fenoterol on the studied immunological parameters, acquire relevance when related to our previous reports showing a depression of the immunological response exerted by theophylline and ketotifen.

Adult↗
PubMed1988

Cytoprotective drugs: a new perspective in the treatment of adverse reactions to foods.

Food allergy (FA) and food intolerance (FI) are complex syndromes caused by adverse reactions to foods. Since mucosal permeability of the digestive tract is often increased during this pathology, we evaluated the clinical efficacy of two different cytoprotective drugs in patients with urticaria-angioedema due to FA and FI. These drugs were pirenzepine, an anti-muscarinic (anti-MI) receptor antagonist, and rosaprostol, a synthetic prostaglandin. Further, the results obtained by these schedules of treatment were compared with those achieved by the previously described polyantihistaminic treatment (ie, the association of anti-H1 plus anti-H2 receptor blockers). The present investigation suggests that the cytoprotective drugs are more effective than the antisecretive ones (ie, the anti-H2). Recently, anti-H2 drugs and ketotifen were shown to be in vitro inhibitors of the immune response and cromolyn was demonstrated capable of exerting an enhancing effect on T cell proliferation. In the present study we tested the effect of pirenzepine on several immunologic parameters, such as lymphocyte proliferation (through different activation pathways) and lymphokine (interleukin-2 and interferon-gamma) production. Since we found that pirenzepine does not affect the immune response and in consideration of its clinical efficacy, we consider this cytoprotective drug a valuable tool in the treatment of adverse reactions to foods.

Adult↗
PubMed1988

Incidence of digestive diseases in patients with adverse reactions to foods.

Adverse reactions to foods may occur when the balance between defensive mechanisms (such as enteric and gastric secretions, digestive enzymes, enteric barrier, etc) and aggressive factors (ie, gastroenteric mucosal damage, constipation, sIgA deficiency, etc) is altered. We therefore analyzed a group of patients with urticaria/angioedema syndrome due to food ingestion evaluating the association with digestive diseases. Our data provide clear evidence for high incidence of concomitant gastroenteric disorders in patients affected by adverse reactions to foods. We consider that these pathologic conditions may promote adverse reactions to foods.

Adolescent↗
PubMed1987

Bacampicillin and the immune response.

To evaluate the possible influence of a bacampicillin on the immune response, 16 subjects, out of a group of 60 patients with bacterial respiratory tract infections, had various tests of immune function determined, before and after treatment. The peripheral mononuclear blastogenic index (ratio between PHA-induced and spontaneous proliferation), PHA-induced interferon-gamma production, percentages of T and B lymphocytes, serum immunoglobulins (IgG, IgM, IgA) levels, failed to show any significative differences before and after the treatment with bacampicillin. The PHA-induced interleukin-2 production increased after treatment but just failed to reach statistical significance (0.1 less than P less than 0.05; t = 1.9). The clinical condition of 56 of the sixty (93.3%) treated patients improved and neither side-effects nor alterations of liver or kidney function were observed. This study has shown no inhibitory effect of bacampicillin on the immune response while confirming its clinical efficacy.

Adolescent↗
PubMed1987

T cell activation surface markers and autologous mixed lymphocyte reaction do not differ in true and pseudo food allergy.

Eighteen patients affected by itching, urticaria, eczema, angioedema, and asthma related to food-stuff intake were studied and classified in two groups (true food allergy and pseudoallergy) on the basis of clinical data, skin prick tests, total and specific IgE levels (PRIST and RAST) and double-blind challenge test. Autologous mixed lymphocyte reaction (AMLR) and T cell activation markers were thought to be tests possibly useful to discriminate between 'true' food allergy and 'pseudoallergy'. The present study failed to show either a significant increase in T cell activation markers (MLR4, Ia) or a significant decrease in AMLR proliferation in such subjects as compared to normal controls. In addition, we found no differences between 'true' allergic and 'pseudoallergic' patients on the basis of the parameters evaluated. Although the AMLR defect was reported both in asthma and in dermatitis, and therefore was thought to be related to atopy, the present data do not confirm this hypothesis in 'true' food allergy.

Adult↗
PubMed1987

Pharmacologic treatment of adverse reactions to foods: comparison of different protocols.

The authors evaluated four different therapeutic protocols employing 80 adult patients with clinical symptoms (urticaria and/or angioedema) due to food ingestion. Thirty-seven patients were allergic. In these cases the diagnosis was made on the basis of simultaneous positivity of the following criteria: history, exclusion diet and challenge tests, positive prick tests, and a positive PRIST and RAST. The other 43 subjects were considered to have pseudo-allergic reactions (PSAR). The pharmacologic treatment was performed for 4 to 6 weeks and each patient had a free diet during the study. At the end of the treatment, both the patient and the allergist filled a score questionnaire concerning the clinical status in order to evaluate the clinical improvement. Our data indicate that anti-H1 antihistamines do not significantly differ from placebos, that oral cromolyn is more successful in food allergy than in PSAR patients (P less than .05), that ketotifen is helpful, and that the association of H1 and H2 anti-histamine drugs is even more effective. Finally, by considering the effect on the immune system exerted by these drugs, as recently demonstrated in our laboratory, a detailed balance of each therapeutic protocol is then analyzed.

Adolescent↗