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Biomedical subjects

G Cimasoni

Publications and source records attributed to G Cimasoni.

At least 55 records · Page 3Linked to original sources
PubMed1984

Preservation of structural specificity in free gingival grafts. Long-term histological observations in man.

Most of the previous histological studies devoted to free gingival grafts in humans were limited to the description of the various phases of healing between the graft and the subjacent tissues. The present long-term study illustrates the histological findings 1 to 36 months after grafting along the zone of passage between graft and surrounding tissues. In the transitional zone between graft and alveolar mucosa it was found that the structural characteristics of the palatal mucosa, with its dense network of collagen fibres and its orthokeratinized superficial layer were conserved even after an interval of time as long as 16 months. These features contrasted with the loose network of collagen fibres and the presence of numerous elastic fibres in the alveolar mucosa. As for the transitional zone between graft and gingiva the findings confirmed that these two types of mucosa are similar, except that the gingival epithelium showed, in epon embedded sections, a layer of parakeratinization, as opposed to the dense layer of orthokeratin found on the grafted palatal epithelium. A constant feature in the present investigation was the change in orientation of the collagen fibres along the zones of healing, both between graft and alveolar mucosa and between graft and gingiva.

Alveolar Process↗
PubMed1984

[Lytic effect of elastase and the granular fraction of human polymorphonuclear leukocytes in the gingival tissue. Biochemical study].

Gingival biopsies were divided in two groups according to their degree of gingival inflammation. They were frozen, sectioned in a cryostat and lyophilized. They were then incubated for 24 hours in the presence of increasing amounts of granular fraction or elastase from human polymorphonuclear leukocytes. The concentrations of hydroxyproline and uronic acid were measured in the supernates. The results show, on one side, an increasing liberation of hydroxyproline in the presence of increasing enzyme concentrations and, on the other side, a greater liberation of hydroxyproline from inflamed as compared to sound gingiva.

Collagen↗
PubMed1983

Longitudinal radiographical study on the rate of alveolar bone loss in patients of a dental school.

Two sets of radiographs, taken at an interval of 4-14 years in 105 patients of a dental school, were examined in order to determine the rate of resorption of alveolar bone. The percentage loss of maximum alveolar bone height was measured in the interproximal and interradicular sites by means of the Schei ruler. The average percentage of interproximal bone resorption was 0.51% (0.07 mm) per year. Age, sex, professional status, type of periodontal treatment and general state of health did not seem to affect the annual resorption rate. However, crowned teeth had a significantly greater rate of resorption than uncrowned teeth. Loss of bone tended to be more rapid in interdental as compared to interradicular areas. A positive and statistically significant correlation was found between initial resorption and annual tooth loss.

Adolescent↗
PubMed1983

Periodontal status associated with chronic neutropenia.

A case of chronic neutropenia in a 12-year-old boy is reported. The patient presented with severe gingival inflammation and alveolar bone loss. Immunologic analysis of the patient's serum revealed the presence of precipitating antibodies against antigenic components of Actinobacillus actinomycetemcomitans Y4 and 652. It was also found that the serum neutralized the leukotoxic activity of Actinobacillus actinomycetemcomitans Y4. The etiology and the pathogenesis of periodontal disease in neutropenic patients are discussed in view of these findings.

Agranulocytosis↗
PubMed1983

Cementogenesis and soft tissue attachment after citric acid treatment in a human. An electron microscopic study.

The four maxillary incisors and two maxillary premolars of a 25-year-old male patient were used to study epithelial and connective tissue attachment 67 days and 164 days after flap surgery and cutting of an horizontal intradentinal groove near the buccal cervical region. Three teeth were topically conditioned for 3 minutes with citric acid pH = 1. The three other teeth were used as controls. The histologic examination was carried out in double-blind conditions; the examiners did not know which specimens were acid treated until the end of the study. Two of the three cases treated with citric acid showed improved healing conditions, when compared to the controls; a more coronal position of the epithelial attachment in the dentin nick as well as a relatively important gain in connective tissue attachment. Two types of connective tissue attachment were observed. The first consisted of an attachment to dentin, without cementum formation and was characterized by a mineralization of decalcified dentin collagen spliced with collagen, newly secreted by fibroblasts. The second type involved cementum formation. Topical citric acid treatment, however, can not be considered as a completely reliable clinical procedure since in one experimental case the type of attachment observed was not better than that seen in the control.

Adult↗
PubMed1982

Alpha 2-macroglobulin in sulci from healthy and inflamed human gingivae.

The concentration of alpha 2-macroglobulin (alpha 2-M) in human gingival sulci has been investigated in two studies: first, in gingival washings during a 21-day period of experimental gingivitis in eight human volunteers and, second, in crevicular fluid collected with filter paper strips before and after initial periodontal therapy in 11 patients. The concentration of total alpha 2-M was found to increase in the washings of the volunteers throughout the period of experimental gingivitis. In the group of patients receiving periodontal therapy, the absolute amount of alpha 2-M in the fluid showed a significant decrease after therapy. The gingival index of inflammation and the crevicular fluid flow also decreased significantly. The specific content of the inhibitor (micrograms of alpha 2-M per mg of fluid per min), however, was found to increase in the fluid with decreasing inflammation. As detected by crossed immunoelectrophoresis, the fluid collected in these patients before therapy, in the presence of severe inflammation, invariably showed peaks of both free and complexed alpha 2-M. In contrast, the fluid collected from the same sites after healing of the inflammation contained no detectable free alpha 2-M.

Gingiva↗
PubMed1980

Collagen breakdown by gingival collagenase and elastase.

The granule fraction of human polymorphonuclear leucocytes (PMNs), the concentrated product of gingival washing from 2 human volunteers and the culture fluid of samples of human gingiva were incubated with neutral salt soluble collagen from rat skin and the patterns of collagen degradation were studied by SDS polyacrylamide gel electrophoresis. Collagenase from human gingiva cleaved the collagen molecules in a fashion similar to that of the PMN granule fraction. Collagen was also attacked by elastase from human PMNs and, to a lesser extent, by elastase from the gingival washings.

Adult↗
PubMed1980

[Lytic effect of human leukocytic proteases on gingival tissue].

Sections of human gingiva obtained in a cryostat have been treated with the granular fraction of human polymorphonuclear leucocytes (PMNs) at pH 3,6 and 7,3, as well as with the enzyme elastase (neutral protease) purified from the same cells. The sections treated by the enzymes have been compared to control serial sections incubated in the absence of enzyme or with the enzyme in the presence of a specific inhibitor. The results showed that the granular fraction at an acid pH seems to affect primarily the connective tissue, while the same fraction at a neutral pH, like the enzyme elastase exerts a preferential action upon epithelium structures.

Connective Tissue↗