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Biomedical subjects

G Chen

Publications and source records attributed to G Chen.

At least 901 records · Page 50Linked to original sources
PubMed1982

The role of the human caudate nucleus in acupuncture analgesia.

The role of the caudate nucleus in acupuncture analgesia was studied in 17 patients receiving caudate stimulation through chronic implanted electrodes to relieve intractable pain caused by late malignancy. Electrical stimulation of the head of the caudate nucleus provided relief from intractable pain in all 17 patients. The pain and pain tolerance threshold were elevated, the alteration in skin galvanic activity, respiratory movement and fingertip plethysmography elicited by a given painful stimulus were depressed. The analgesia required a period of induction and persisted for some time after cessation of stimulation; it showed no obvious segmental topography. These characteristics are similar to those observed in acupuncture analgesia. The effect of caudate stimulation was similar to that of electric needling in depressing the late component of the somatosensory evoked potentials (SEPs) recorded over the scalp and from the centrum medianum of the thalamus. Evoked potentials could be recorded from the caudate nucleus when an acupuncture point was electrically stimulated. The evoked potential record was a complex wave of positive and negative components, the peak latencies of main components being 100-180 msec for positive wave and 148-332 msec for negative wave components. The present study supports the supposition that the caudate nucleus may play a role in acupuncture analgesia. The possibility that the effect of caudate stimulation is brought on by inhibiting the activity of the medial thalamus is discussed.

Acetylcholine↗
PubMed1975

Spectrofluorometric determination of the antibiotic lasalocid in blood.

A spectrofluorometric assay was developed for the determination of the antibiotic lasalocid in dog blood, based on the intrinsic fluorescence of the compound in ethyl acetate. The assay can measure "total" levels of drug and any metabolites present. The specificity of the assay was verified by TLC separation of the dog blood extract, which indicated the presence of only intact drug. The overall recovery (+/- SD) of lasalocid was 62.0 +/- 3.6% in the concentration range of 1.0-10 mug of compound/ml of dog blood. The sensitivity of the assay is 0.5 mug/ml. The assay was applied to the determination of blood levels of lasalocid in the dog following the intravenous administration of a 5-mg/kg dose.

Animals↗
PubMed1975

Control of the mRNA for hepatic tryptophan oxygenase during hormonal and substrate induction.

Glucocorticoid hormones increase the level of hepatic tryptophan oxygenase (EC 1.13.11.11; L-tryptophan:oxygen 2,3-oxidoreductase (decyclizing) by increasing its rate of synthesis. Studies were performed to determine whether this induction is mediated by controlling the level of the mRNA for tryptophan oxygenase of by changing the translational efficiency of a fixed level of mRNA. Activity of tryptophan oxygenase mRNA was quantitated in a Krebs ascites cell-free, protein-synthesizing system, supplemented with tRNA and rabbit reticulocyte initiation factors. De novo synthesis of the protomeric unit(s) of the enzyme was a linear function of the amount of mRNA added. Time course and dose-response studies in which the enzyme level and mRNA activity in livers from rats injected with inducing doses of hydrocortisone were compared indicate that the induction of this enzyme is accompanied by a proportional increase in the level of its mRNA. This was true for mRNA isolated from total liver as well as from cytoplasmic polysomes. Induction of the enzyme by its substrate, tryptophan, however, was not accompanied by a parallel increase in mRNA activity.

Animals↗
PubMed1975

Neurohumors in acupuncture.

In acupuncture an increase of some substance at the reactive site seems to be required in order to have a biochemical response. Among the substances to be considered are the neurohormones. Experimentally, the sympathetic and parasympathetic neurohormones are shown to be involved in analgesia and in the transmission of nerve impulses.

Acetylcholine↗