Biomedical subjects

G C Yang

Publications and source records attributed to G C Yang.

76 records · Page 5Linked to original sources

PubMedDate not supplied

Ultrafast Papanicolaou stain. An alternative preparation for fine needle aspiration cytology.

The objective of this study was to develop a Papanicolaou stain as fast as Diff-Quik yet with cytomorphology as exquisite as that processed by ThinPrep for the optimal evaluation of fine needle aspirates. Satisfactory results were obtained after three modifications were made: (1) rehydration of air-dried smears with normal saline, (2) use of a 4% formaldehyde/65% ethanol fixative, (3) and use of Richard-Allan Hematoxylin 2 and Cyto-stain. The first modification restored the transparency of the cells and hemolysed red blood cells, the second modification reduced the time needed for proper fixation and staining from minutes to seconds, and the third modification simplified the procedure. This 90-second protocol yields a transparent, polychromatic stain with crisp nuclear and cytoplasmic features. The cytomorphology processed by this protocol is at least equal to, if not better than, the quality of specimens prepared by ThinPrep and superior to those processed by the standard Papanicolaou procedure.

Azure Stains↗

PubMedDate not supplied

Fine needle aspiration cytology of low grade endometrial stromal sarcomas.

The cytomorphology of endometrial stromal sarcoma (ESS) has been described. However, almost all of the reports are about the high grade type according to the newly revised histopathologic criteria; the cytomorphology of low grade ESS (endolymphatic stromal myosis) has yet to be characterized. This paper reports on two cases of recurrent low grade ESS diagnosed by fine needle aspiration and confirmed histopathologically. The aspirates consisted of extremely cohesive tissue fragments of monotonous, small (8-9 microns), oval cells tightly woven in a metachromatic, extracellular matrix on Diff-Quik-stained smears. In Papanicolaou-stained smears the transparency allowed visualization of bland, normochromatic, oval nuclei as well as small arterioles transecting the tissue fragment, whereas the extracellular matrix became invisible. The metachromatic, extracellular matrix in the smears correlated with the reticular fibrillar network wrapping around individual tumor cells on tissue sections, one of the histopathologic criteria that separates low grade from high grade ESS.

Abdominal Neoplasms↗

PubMedDate not supplied

Adenomyomatous, lower uterine segment and endocervical polyps in cervicovaginal smears.

To the best of our knowledge, the specific features of polyps in cervicovaginal smears have not been described; that fact prompted us to undertake this study. Fifty-seven cases of polyps below the uterine fundus with cytohistopathologic correlations were analyzed. The tissue diagnoses were 2 cases of adenomyomatous, 2 cases of lower uterine and 53 cases of endocervical polyps. In the cervicovaginal smears, adenomyomatous polyps presented as cohesive fragments with frayed edges, revealing spindle cells with bipolar cytoplasmic processes. Lower uterine segment polyps showed peculiar tissue fragments composed of several fractured arterioles connected by sheets of small stromal cells. Endocervical polyps presented as smooth-bordered, polypoid tissue fragments, lined at the outermost layer with simple columnar cells, sandwiched within a pale intermediate zone and packed at their inner core with numerous small, dark stromal cells. This specific finding, however, was observed in only one case. The remaining 52 patients had no specific findings in their smears.

Adenomyoma↗

PubMedDate not supplied

Merkel cell tumor-like neuroendocrine carcinoma associated with the submandibular gland. Report of a case with cytologic, immunohistochemical, electron microscopic and flow cytometric studies.

We report a rare case of primary Merkel cell tumor (MCT)-like neuroendocrine carcinoma (i.e., small cell carcinoma) associated with the submandibular gland in a 73-year-old man. Despite the lack of a known skin primary, the tumor exhibited cytomorphologic (predominant single cells with "intermediate filament buttons" in the background), ultrastructural (dense core granules and a globular aggregate of intermediate filaments) and immunohistochemical (paranuclear globular coexpression of cytokeratin and neurofilaments) features identical to that of MCT. The tumor contained an aneuploid peak with DNA index of 1.1 and an S phase of 20.5%. The tumor measured 5.8 cm and was situated between the skin and submandibular gland, with which it was closely associated but separated by connective tissue. The literature on MCT without a known skin primary is reviewed, and the cytomorphologic features that allow the distinction between MCT, lymphoma and oat cell carcinoma are discussed.

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