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Biomedical subjects

G C Townsend

Publications and source records attributed to G C Townsend.

At least 19 recordsLinked to original sources

Symmetry and perceived facial attractiveness: a monozygotic co-twin comparison.

Symmetry is a major correlate of physical attractiveness across species, including humans. Investigating the nature of this relationship has been difficult, however, for several reasons, including the facts that variance in symmetry is attributable to more than one source and is often correlated with other variables related to attractiveness. This study assessed the role of facial symmetry in relation to perceptions of facial attractiveness. Some of the natural covariates of symmetry were controlled for by comparing the symmetry and attractiveness differentials between monozygotic co-twins, who are genetically, but not developmentally, identical. The more symmetric twin of a pair was consistently rated as more attractive, and the magnitude of the difference between twins in perceived attractiveness was directly related to the magnitude of the difference in symmetry.

Adult

Digastric muscle of the kangaroo: a comparative anatomical study.

Although the human digastric muscle is classified as a suprahyoid muscle, none of the digastric muscles in other mammals are classed as suprahyoid in textbooks of veterinary anatomy. The aim of this study was to describe the anatomical relationship of the digastric muscle in a marsupial, the kangaroo, and to consider factors thought to be important in leading to the different position of the muscle in quadrupeds compared with humans. Eight heads of the common wallaroo (Macropus robustus) were used in this study. They were fixed by injection of 10% formalin solution into the carotid arteries and dissected under a stereomicroscope. The digastric muscle in the common wallaroo arose from the paroccipital process of the temporal bone and inserted into the mandible but had no intermediate tendon or any connection with the hyoid bone. It was supplied by both the mandibular and facial nerves. The hyoglossus muscle attached to the inferior surface of the hyoid bone and its ventral border overlapped the mylohyoid muscle. The hypoglossal nerve passed between these two muscles. Therefore, in contrast to humans, the digastric, hyoglossus, and mylohyoid muscles in the kangaroo were all located inferior to the hyoid bone. Differences in head posture and the position of the larynx between kangaroos and humans may account for the observed difference in the digastric muscle's position relative to the hyoid bone between these species.

Animals

Genetic aspects of dental disorders.

This paper reviews past and present applications of quantitative and molecular genetics to dental disorders. Examples are given relating to craniofacial development (including malocclusion), oral supporting tissues (including periodontal diseases) and dental hard tissues (including defects of enamel and dentine as well as dental caries). Future developments and applications to clinical dentistry are discussed. Early investigations confirmed genetic bases to dental caries, periodontal diseases and malocclusion, but research findings have had little impact on clinical practice. The complex multifactorial aetiologies of these conditions, together with methodological problems, have limited progress until recently. Present studies are clarifying previously unrecognized genetic and phenotypic heterogeneities and attempting to unravel the complex interactions between genes and environment by applying new statistical modelling approaches to twin and family data. Linkage studies using highly polymorphic DNA markers are providing a means of locating candidate genes, including quantitative trait loci (QTL). In future, as knowledge increases; it should be possible to implement preventive strategies for those genetically-predisposed individuals who are identified to be at risk.

Chromosome Mapping

Wear of human enamel: a quantitative in vitro assessment.

Many factors influence the extent and rate at which enamel wears. Clinical studies in humans are limited by difficulties in the accurate quantification of intra-oral wear and by a lack of control over the oral environment. The purpose of this study was to determine the wear characteristics of human dental enamel under controlled experimental conditions. An electro-mechanical tooth wear machine, in which opposing enamel surfaces of sectioned, extracted teeth were worn under various conditions, was used to simulate tooth grinding or bruxism. Enamel surface wear was quantified by weight to an accuracy of 0.1 mg, with water uptake and loss controlled. The variables considered included the structure and hardness of enamel, facet area, duration of tooth contact, relative speed of opposing surfaces, temperature, load, pH, and the nature of the lubricant. Enamel wear under non-lubricated conditions increased with increasing load over the range of 1.7 to 16.2 kg. The addition of a liquid lubricant (pH = 7) reduced enamel wear up to 6.7 kg, but when the load increased above this threshold, the rate of wear increased dramatically. With the viscosity of the lubricant constant and pH = 3, the rate of wear was further reduced to less than 10% of the non-lubricated rate at 9.95 kg, after which the rate again increased substantially. Under more extreme conditions (pH = 1.2, simulating gastric acids), the wear was excessive under all experimental loads. When saliva was used as a lubricant, the amount of wear was relatively low at 9.95 kg, but rapid wear occurred at 14.2 kg and above. These results indicate that under non-lubricated conditions, enamel wear remains low at high loads due to the dry-lubricating capabilities of fine enamel powder. Under lubricated conditions, low loads with an acidic lubricant lead to little enamel wear, whereas very low pH results in a high rate of wear under all loads.

Analysis of Variance

Morphological variability of the human inferior alveolar nerve.

The aims of this study were: to compare the radiographic appearance of the neurovascular bundle and its surrounding bone with the actual situation revealed on dissection; to reveal the morphology of the neurovascular bundle in dentate and edentulous subjects; and to note any changes occurring following the removal of the teeth and the consequent resorption of alveolar bone. Twenty-nine human mandibles were examined; 20 were dry skeletal specimens that were examined radiographically and the remaining nine were radiographed then dissected after decalcification and the branches of the inferior alveolar nerve were identified, sketched, and photographed. Different patterns of identifying characteristics of the mandibular canal were noted on the radiographs, ranging from alternating bands of radiopacity and radiolucency to continuous radiopaque lines. Dissections indicated that the radiographic appearance related to the number, distribution, and pattern of trabeculae around the canal. The dissections revealed that in all cases, the main nerve divided into its incisive and mental branches in the molar area well before reaching the mental foramen. A branch to the molar teeth, and in two instances to the second premolar as well, was given off from the main trunk before it divided into incisive and mental branches. In the dentate specimens, the neurovascular bundle formed two distinct curvatures, one between the mandibular and mental foraminae and the other between the mental foramen and the incisor teeth apices. In the edentulous specimens, the neurovascular bundle was reduced in size and although some small nerve branches, notably to the molar areas, were distinguishable, the blood vessels seemed to have atrophied beyond macroscopic identification. This project confirmed the inaccuracy of descriptions repeated in many anatomical textbooks suggesting that the inferior alveolar branch of the trigeminal nerve divides at the mental foramen into its incisive and mental branches and that during their path through the body of the mandible they give off fibers to the individual teeth. It further confirmed that the neurovascular bundle reduces in size quite markedly after removal of teeth and that the vascular component cannot be clearly identified.

Aged

The new antibiotics, 1997.

Some of the antimicrobial agents released in the past few years possess characteristics suggesting real advantages over older agents (see Table I). These advantages include broader spectrum of activity, favorable pharmacokinetic profile allowing for infrequent dosing, low toxicity and lower pricing. Although older agents and agents with narrower spectra should be considered first-line in most circumstances, there may be some clinical scenarios in which these newer agents should be contemplated as reasonable alternatives.

Anti-Bacterial Agents

The use of corticosteroids in the management of bacterial meningitis in adults.

Despite the introduction of newer antimicrobial agents, bacterial meningitis continues to be associated with significant morbidity and mortality. Evidence from in-vitro studies, experimental animal models, and clinical studies indicate that the host inflammatory response is responsible for much of the deleterious consequences of this disease. Thus, there is much interest in the adjunctive use of antiinflammatory agents in the therapy of bacterial meningitis. Although there is considerable evidence from animal models and from clinical trials in children that adjunctive antiinflammatory therapy with corticosteroids is effective in reducing inflammation and in improving long-term outcomes, similar data involving adults are largely lacking. The rationale for the use of corticosteroids in the management of bacterial meningitis, and the applicability to disease in adults, are discussed, and some recommendations for their use in this setting are made.

Adrenal Cortex Hormones

In vitro models of the blood-brain barrier to study bacterial meningitis.

In vitro models of the blood-brain barrier involving culturing cerebral microvascular endothelial cells may provide information critical to understanding diseases of the central nervous system, such as bacterial meningitis, that would be difficult to obtain from clinical studies or from in vivo models. These models may also identify targets for therapeutic intervention.

Animals

Genetic and environmental influences on facial profile.

Observed variation in facial morphology results from the influences of both genetic and environmental factors. To investigate the role of genetic influences on variation in facial profile, lateral photographs of 23 male and 19 female monozygous (MZ), and 15 male and 22 female dizygous (DZ) twin pairs were selected from the records of the South Australian Twin Study. Soft tissue landmarks and facial profiles were identified, traced and digitized. Linear and angular measures defined by the landmarks were calculated and a series of Fourier functions derived to describe the facial profiles. These Fourier amplitudes were interpreted in terms of the specific regions which they represented. Correlations within MZ pairs were consistently higher than within DZ pairs for both the facial dimensions and the Fourier amplitudes, providing evidence of significant genetic contribution to facial convexity, facial height and facial depth. Variability in nose and lip morphology appeared to be under stronger environmental influence.

Adolescent

Potential role of nitric oxide in the pathophysiology of experimental bacterial meningitis in rats.

We have investigated the possible role of nitric oxide (NO) in the pathophysiology of bacterial meningitis (BM) by using the rat model of experimental BM. The nitrite concentration in cerebrospinal fluid (CSF) was used as a measure of NO production in vivo since NO rapidly degrades to nitrite and nitrate. Rats were inoculated intracisternally with live bacteria (5 x 10(6) CFU of Haemophilus influenzae type b strain DL42 or Rd-/b+/O2), with bacterial endotoxin (20 ng of DL42 lipooligosaccharide [LOS] or 200 ng of Escherichia coli lipopolysaccharide), or with a saline control vehicle. CSF samples were collected preinoculation and at the time of maximal alteration in blood-brain barrier permeability (BBBP). CSF [nitrite] was quantified by measuring A550 after addition of the Greiss reagent and comparison to a standard curve of sodium nitrite. Rats inoculated with either DL42, Rd-/b+/O2, LOS, or lipopolysaccharide demonstrated a significantly elevated mean peak CSF [nitrite] (8.34, 15.62, 10.75, and 10.44 mM, respectively) versus the concentration prior to treatment and/or those in saline-treated animals (5.29 and 5.33 mM, respectively; P < 0.05 for each comparison). We then determined if there was a correlation between CSF [nitrite] and percent BBBP (%BBBP) at various time points postinoculation with Rd-/b+/O2. %BBBP was defined as the concentration of systemically administered 125I-labeled bovine serum albumin in the CSF divided by the level of 125I-labeled bovine serum albumin in serum multiplied by 100. The mean %BBBP increased in tandem with the mean CSF [nitrite] (R = 0.84, P = 0.018), which peaked at 18 h in the absence of a change in the serum [nitrite]. Systemic administration of the NO synthase inhibitor N-nitro-L-arginine methyl ester demonstrated a significant reduction of mean CSF nitrite production (0.95 versus 6.0 mM in controls; P = 0.02) when administered intravenously to animals which had been inoculated intracisternally with 20 ng of LOS. Suppression of mean leukocyte pleocytosis (3,117 versus 11,590 leukocytes per mm3 in control LOS-challenged rats; P = 0.03) and mean alterations of BBBP (2.11 versus 6.49% in control LOS-challenged rats; P = 0.009) was observed concomitantly with decreased CSF [nitrite]. These results support the hypothesis that NO contributes to increased %BBBP in experimental BM.

Animals

Genetic covariance structure of incisor crown size in twins.

Previous studies of tooth size in twins and their families have suggested a high degree of genetic control, although there have been difficulties separating the various genetic and environmental effects. A genetic analysis of variation in crown size of the permanent incisors of South Australian twins was carried out, with structural equation modeling used to determine the relative contributions of genetic and environmental factors. Maximum mesiodistal crown dimensions of maxillary and mandibular permanent incisors were recorded from dental models of 298 pairs of twins, including 149 monozygous (MZ) and 149 dizygous (DZ) pairs. The analysis revealed that: (i) an adequate fit required additive genetic and unique environmental components; (ii) augmenting the model with non-additive genetic variation did not lead to a significant improvement in fit; (iii) there was evidence of shared environmental influences in the upper central incisors of males; (iv) the additive genetic component constituted a general factor loading on all eight teeth, with group factors loading on antimeric pairs of teeth; (v) unique environmental effects were mostly variable-specific; (vi) most factor loadings on antimeric tooth pairs could be constrained to be equal, indicating a symmetry of genetic and environmental influences between left and right sides; and (vii) estimated heritability of the incisor mesiodistal dimensions varied from 0.81 to 0.91.

Adolescent

Platelet-activating factor augments meningeal inflammation elicited by Haemophilus influenzae lipooligosaccharide in an animal model of meningitis.

Research into the pathophysiology of bacterial meningitis has suggested a role for various endogenous inflammatory mediators, such as platelet-activating factor (PAF). In the present study, rats were inoculated intracisternally with various doses of PAF, with Haemophilus influenzae lipooligosaccharide (LOS) in high doses (20 ng) alone, and with a low dose of LOS (200 pg) with or without low doses of PAF (25 ng to 2.5 micrograms). Values for cerebrospinal fluid leukocytosis and percent blood-brain barrier permeability to systemically administered 125I-labeled albumin observed after inoculation of low-dose LOS with PAF were greater (P < 0.05) than those observed after inoculation of low-dose LOS alone and not statistically different from those observed after inoculation of high-dose LOS. PAF alone elicited an inflammatory response only at high doses (25 micrograms). These results support the hypothesis that low cerebrospinal fluid PAF concentrations, such as those observed in children with bacterial meningitis, may augment the inflammatory response to the presence of bacteria in the subarachnoid space.

Animals

The endalveolar crest: anatomic and prosthodontic considerations of an overlooked mandibular landmark.

Surgical and prosthodontic treatment of edentulous patients have confirmed the presence of a seldom-described crest of bone situated above and distal to the mylohyoid ridge of the mandible. This study aimed to establish the frequency of occurrence and range of expression of the feature, which it is suggested should be called the endalveolar crest in humans and other primates. Evidence of the crest was noted in all specimens examined although the expression varied. Dissection of fresh and preserved cadavers indicated that the crest provided attachment for part of the superior constrictor muscle and that it may also afford some protection to the lingual nerve in the retromolar region.

Alveolar Process

Adjunctive therapy for bacterial meningitis: rationale for use, current status, and prospects for the future.

Despite the introduction of numerous therapeutic advances, the morbidity and mortality associated with bacterial meningitis remain significant. Research into the pathophysiology of bacterial meningitis has revealed that the inflammatory response resulting from bacterial invasion of the subarachnoid space is due in large part to the activity of host-derived mediators. This inflammatory response is ultimately responsible for the long-term neurological sequelae and death associated with bacterial meningitis. In vitro and in vivo models of bacterial meningitis have identified several points in the inflammatory cascade that may be amenable to therapeutic intervention. Numerous potential therapeutic agents that may limit inflammation of the subarachnoid space have been and are being developed, and trials in animal models and in humans are under way. The judicious use of safe and effective agents with demonstrated efficacy as adjuncts to bactericidal antimicrobial agents in the therapy for bacterial meningitis in humans may improve the prognosis of this disease.

Adrenal Cortex Hormones

Craniofacial patterning in Klinefelter (47 XXY) adults.

Radiographic cephalometry incorporating a pattern profile analysis was used to compare 26 adult Klinefelter males from Finland with first degree adult relatives, 15 males and 14 females. Compared with female relatives, the 47 XXY males were larger in almost all craniofacial linear dimensions, but were similar in facial shape apart from greater mandibular prognathism. Mandibular dimensions in particular differed between the Klinefelter and unaffected males, the corpus length being larger, the ramus shorter and the gonial angle more obtuse in the 47 XXY group. The prominent facial profile, most marked in the mandible, was a dominant feature of the Klinefelter subjects who also displayed a more acute median cranial base angle than each control group. Generally, Klinefelter morphology was marked by greater variability or patterning of the craniofacial structures compared with relatives, possibly due to decreased developmental canalization. It is proposed that the 47 XXY complex may affect endochondral growth in the cranial base, as well as having a direct influence on jaw growth.

Adolescent