Biomedical subjects
G C Jenkins
Publications and source records attributed to G C Jenkins.
Evaluation of the in vitro antiproliferative properties of four novel anthracyclines YM1, 3, 4 and 6 in human leukemia cell lines.
The antitumor activity of novel doxorubicin analogues YM1, YM3, YM4 and YM6 was evaluated against drug sensitive U937 monocytic leukemia and CCRF-CEM lymphoid leukemia cell lines, as well as drug resistant CEM/VLB100 lymphoid multidrug resistant leukemia cell line by a [3H]thymidine incorporation assay. Different antileukemic activities of these new anthracyclines were observed in our studies. These novel anthracyclines produced a dose- and time-dependent inhibition in all the leukemic cell lines tested, while YM1 and YM3 were more effective than YM4 and YM6 against all the leukemic cell lines. The antitumor activity of all these novel analogues was lower than that of doxorubicin or epidoxorubicin in drug sensitive leukemic cells. The relative resistance values (IC50 of resistant cell line/IC50 of sensitive parental cell line) of YM1, 3, 4 and 6 were 27, 7, 5 and 14 respectively. These were lower than the resistance values for ADM and EDR which were 45 and 40 respectively. YM3 had a similar antileukemic activity against the CEM/VLB100 drug resistant leukemic cell line to ADM or EDR with a lower relative resistance value and a slightly increased IC50 value. Our results suggest that YM3 may be used in high dose for the clinical treatment of leukemias with possible less cardiotoxicity as well as less drug resistance.
Recent medico-legal problems in haematology.
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A comparative study of intravenous ciprofloxacin and benzylpenicillin versus netilmicin and piperacillin for the empirical treatment of fever in neutropenic patients.
We examined the efficacy of ciprofloxacin as an empirical treatment for fever in 97 neutropenic patients in a randomized study of ciprofloxacin and benzylpenicillin versus netilmicin and piperacillin. Benzylpenicillin was included because of evidence of in-vitro resistance to ciprofloxacin in some streptococci. Clinical response rate was similar in the two groups (46% resolution for ciprofloxacin/benzylpenicillin and 52% for netilmicin/piperacillin). Microbiological assessment revealed more pathogens eradicated by ciprofloxacin and benzylpenicillin (66%) and fewer persisting (3%) than in patients receiving netilmicin and piperacillin (52% and 13% respectively). Staphylococcus epidermidis was the commonest pathogen, accounting for 38% of all isolates and 30% of all treatment failures. There were no treatment failures or superinfections due to streptococci. More therapy-related adverse reactions were seen in patients on netilmicin and piperacillin (28%) compared with those on ciprofloxacin and benzylpenicillin (10%). The combination of ciprofloxacin and benzylpenicillin is as effective as a standard regimen of netilmicin and piperacillin, with fewer adverse effects, and is highly attractive as empirical therapy for the febrile, neutropenic host. The inclusion of benzylpenicillin prevents streptococcal-associated treatment failure.
Abnormal features of peripheral blood films from Gambian children with malaria.
A series of peripheral blood films taken from Gambian children with either acute or low-grade Plasmodium falciparum infections were examined for abnormal features of the red and white cells. Hypochromia and polychromasia with cytoplasmic stippling were predominant features in both groups. Lymphocytosis, granulocytosis and plasmacytosis were common white cell abnormalities. An additional feature in films from patients with acute malaria was the presence of numerous atypical lymphocytes. A comparison of the features in the two groups indicated that some abnormalities are associated with an acute attack of malaria and that others have a nutritional or genetic aetiology.
Sickle cell trait.
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Clinical psychologists in general practice.
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A narrow escape from splenectomy.
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Care of children in general practice.
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Trypanosoma brucei: renal pathology in rabbits.
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The advantage of a build-up anti-globulin technique for the detection of immunoglobulin on the red cells of rabbits infected with trypanosomes. A preliminary report.
A build-up anti-globulin technique has been used to detect immunoglobulin on the red cells of rabbits infected with Trypanosoma brucei brucei S 42 and 427. The method, which is useful for the detection of immunoglobulin on sparsely coated red cells, involves treating the red cells of infected rabbits with antiglobulin followed by alternate globulin and antiglobulin layers until a lattice is built up which allows agglutination to occur. The red cells of five infected rabbits have been tested with anti-rabbit IgG,-IgM and broad spectrum anti-globulin reagents at intervals after infection and following the administration of Berenil, a drug which destroys trypanosomes. The positive anti-globulin reactions which developed following inoculation with trypanosomes were much reduced after giving Berenil. The tests became more strongly positive again in two rabbits after reinfection.
Regional co-ordinator for haemophilia in domiciliary practice.
The North-east Thames Region has appointed a nursing sister to co-ordinate the organisation of care for haemophiliacs in the region. As a result of the appointment, facilities for home treatment have expanded rapidly. Several associate centres providing care to haemophiliacs have been set up around the region in addition to the four main haemophilia centres, which are all in the south-west corner of the region. As well as providing support and supervision of patients on home treatment, the co-ordinator helps to place haemophiliac children in suitable schools, maintains the regional register of haemophiliacs, and has a more general role in ensuring that services are available where they are needed throughout the region.
Developmental and paediatric care of the pre-school child.
Through an Upjohn Travelling Fellowship I visited 27 experts in childcare and sought their opinions on the privileges, possibilities, and problems in organising developmental and paediatric care for pre-school children in the United Kingdom.The role of the general practitioner was seen by many of the experts clearly. How he is to play it is shrouded in uncertainty. Research is urgently needed both on the tools of surveillance and on the different methods of arranging care.
The clinical, haematological and bacteriological effects of long-term treatment with co-trimoxazole.
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Mechanized blood grouping: a hospital trial using an 8-channel grouping machine.
ABO and Rh (D) groups of 6403 blood samples were assessed on an 8/9-channel autoanalyser in the Serology Department of the London Hospital; the results were independently checked at the Regional Blood Transfusion Centre, Brentwood, using the routine methods for grouping donor blood. Results of this comparative study are given and instances are described in which anomalous results or incorrect groupings occurred; the possible causes are discussed. The 8/9-channel automated blood group analyser is evaluated in terms of routine hospital laboratory practice.
Blood group, serum protein and red cell enzyme polymorphisms in a population from the Seychelle Islands.
161 inhabitants of the Seychelle Islands have been examined for blood group, serum protein, and red cell enzyme polymorphisms. The gene frequency data obtained from this survey supports the anthropological view that the present-day Creole-speaking inhabitants of the Seychelle Islands result from a admixture of African and European stock.
Haematological observations in rabbits infected with T. brucei.
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