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Biomedical subjects

G C Jain

Publications and source records attributed to G C Jain.

At least 19 recordsLinked to original sources
PubMed1998

Effect of oviductal cell co-culture on cleavage and development of goat IVF embryos.

The present study was undertaken with the aim to study the role of isologous and heterologous (buffalo) oviductal cell to co-culture on in vitro development of goat embryos. The oocytes were collected by puncturing the goat ovaries obtained from slaughterhouse. The oocyte recovery rate per ovary was 3.0. The media used for oocyte maturation and embryo development was TCM-199 + 10 percent buffalo estrus serum. A total of 79.8 percent oocytes got matured out of 1056 oocytes. The oocytes were inseminated with epididymal buck spermatozoa capacitated in Brackett and Oliphant media. In group I without oviductal cells co-culture only 13.6 percent matured oocytes cleaved and 3.3 and 0.0 percent reached the morula and blastocyst stage. In group II and III having goat and buffalo oviductal cells the cleavage was 57.6 and 59.2 percent respectively. The percentage of morula, blastocyst and those embryos arrested between 2-16 cells were 26.3, 10.2, 63.5 and 26.6, 8.9 and 64.5 in goat and buffalo oviductal cell groups. The results indicated that the oviductal cell co-culture had a marked effect on cleavage and development of goat IVF embryos. Buffalo oviductal cells can be used well for goat embryo development.

Animals↗
PubMed1997

Oocyte retrieval and histological studies of follicular population in buffalo ovaries.

Ovaries (N = 250) from slaughtered buffaloes were collected to study follicular population and compare methods of oocyte retrieval. The number and size of surface follicles were recorded and grouped into different categories. Different sized follicles in relation to oocyte diameter were studied histologically. Yield of oocytes per ovary were less (P < 0.05) from ovaries bearing a corpus luteum (CL). Techniques used for oocyte recovery included slicing, follicle puncture and aspiration. The oocyte recovery rate was greatest (P < 0.05) using slicing. The average number of visible surface follicles was 5.20 +/- 0.97 with mean numbers of 2.5, 1.2, 0.82 and 0.62 per ovary for follicles sized 4, 8, 12 and 12mm respectively. Histological studies revealed large numbers of primordial follicles in prepubertal and atretic follicles in senile buffaloes. They also established a biphasic relationship of growth between oocyte diameter and follicular size.

Animals↗
PubMed1989

Clinical correlates of acute right ventricular infarction in acute inferior myocardial infarction.

Right ventricular infarction was diagnosed on the basis of ST-segment elevation greater than or equal to 1 mm in at least one right precordial lead (V3R-V6R) in 20 of 50 patients with first acute inferior myocardial infarction. Seventy five percent of these had ST elevation in 2 or more right precordial leads. Giddiness and hiccups were more common amongst such patients (P less than 0.05). Signs of right ventricular dysfunction-raised jugular venous pressure (65%), Kussmaull's sign (45%), hypotension (without cardiogenic shock, 40%) and right-sided third sound (25%) in the absence of clinical left ventricular failure, were noted in 65% of such patients. Eleven patients had 2 or more of the above signs. ST elevation in 2 or more right precordial leads was found in 10 of these 11 patients. A more complicated course in the hospital characterised by bradyarrhythmias, hypotension and cardiogenic shock, combined with a greater mortality was seen in such patients. We conclude that the bedside diagnosis of haemodynamically significant right ventricular infarction can be made on the basis of a combination of clinical signs and ST elevation in 2 or more right precordial leads, even in units not equipped for bedside haemodynamic monitoring, echocardiography and radionuclide studies. This should lead to a better identification and management of such patients.

Acute Disease↗
PubMed1979

Cataractogenous effect of hair dyes: a clinical and experimental study.

Observations based on a clinical and experimental study of the effect of hair dye (paraphenylenediamine) on the lens are reported. Two hundred individuals of either sex using hair dye were examined in detail and lenticular changes were found in 89% while only 23% in controls. The lenticular changes were related to duration, amount, and individual sensitivity to dye. Various types of lenticular opacities encountered are mentioned. In addition some individuals (7%) were found to develop early presbyopia. Experimental study which was conducted in rats and rabbits confirmed the cataractogenic effect of hair dye beyond doubt. It is concluded that hair dye is potentially toxic to the human lens.

Adult↗