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G C Grosveld

Publications and source records attributed to G C Grosveld.

23 records · Page 2Linked to original sources
PubMed1981

A transcription map for the rabbit beta-globin gene.

We have characterized the transcripts of the beta-globin gene in RNA isolated from rabbit bone marrow using the S1 mapping procedure of Berk and Sharp (1977). The largest beta-globin-specific RNA that we have detected is a transcript, colinear with the gene, that contains the mRNA coding sequences plus both intervening sequences but does not extend into the extragenic DNA sequences. The 5' and 3' ends of this pre-mRNA maps at the same position as those of mature globin mRNA. Several partially spliced RNAs are described that are probably intermediates in the pathway from the colinear transcript to mature mRNA. The largest spliced pre-mRNA has lost only about 40 of the 126 nucleotides of the small intervening sequence and contains the entire large intervening sequence together with the coding sequences. A second major intermediate pre-mRNA lacks only the entire small intervening sequence. Finally, a pre-mRNA is described in which all but approximately 90 nucleotides of the large intervening sequence have been excised. Thus both intervening sequences appear to be able to be excised in a stepwise fashion. Removal of the small intervening sequence seems to precede the removal of the large intervening sequence.

Animals↗
PubMed1981

Localization of DNA sequences necessary for transcription of the rabbit beta-globin gene in vitro.

We have studied the transcription in vitro of the rabbit beta-globin gene in the HeLa cell system. Using cloned fragments of this gene, we obtained specific transcripts with 5' ends at the "cap" site. The analysis of the transcription of a large number of deletion mutants has shown that the cap site and the conserved CCAAT box at 75 nucleotides upstream from the cap site are not required for specific in vitro transcription. Sequences localized within the region from 34 to 20 nucleotides upstream from the 5' end of the cap site, however, are required for in vitro transcription. Since the conserved "Goldberg-Hogness" box is localized in this region, we conclude that this is the major requirement for specific transcription in vitro. Finally, in at least eight cases, deletions localized to the 3' side of the Goldberg-Hogness box shift the transcription-initiation site downstream to a position approximately 30 nucleotides from the Goldberg-Hogness box. This is consistent with the idea that RNA polymerase II is bound at the Goldberg-Hogness box and initiates transcription 30 nucleotides downstream from this site. There is apparently little sequence specificity in the selection of the site of initiation.

Animals↗