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Biomedical subjects

G Busnach

Publications and source records attributed to G Busnach.

63 records · Page 4Linked to original sources
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Polymorphonuclear cell phagocytosis and surface receptor modulation after extracorporeal circulation.

Polymorphonuclear cells (PMN) from patients treated with hemodialysis (HD) or plasma exchange (PE) were analyzed by flow cytometry to determine modulation in phagocytic capacity and Fc-gamma and C3bi receptor expression following extracorporeal circulation (EC). Fluorescent microbeads (phi 2.02 m) were used in the evaluation of phagocytosis, and phycoerythrin conjugated Leu11c and Leu15 monoclonals identified Fc-gamma and C3bi receptors respectively. The percentage of positive cells and mean receptor density on PMN surfaces were calculated for each antibody before and after the procedures. Fc-gamma receptor expression was reduced overall in HD and PE cases, but unaffected after EC even with specific paraprotein removal. C3bi receptor was normally expressed on PMNs before and after EC, but receptor density on the cell surface increased, and phagocytosis was qualitatively and quantitatively depressed after EC. The resulting effect of EC on PMNs was therefore a temporary increase in C3bi receptor density after the procedure, which was independent of HD or PE technique, of the primary disease, and of the quality of the PE reinfusion solutions, suggesting a procedure-related effect, and a down-regulation of PMN phagocytic activity. Both effects may be related to membrane biocompatibility.

Antigens, Differentiation↗
PubMedDate not supplied

[The relationship between systolic time intervals and the electrocardiographic pattern in essential hypertension (author's transl)].

Systolic time intervals at rest and after exercise were studied in 56 patients with essential hypertension. The patients were divided into groups according to the presence or absence at rest of electrocardiographic patterns of left atrial hypertrophy, and left ventricular strain. An impaired left ventricular function was shown, at rest and after exercise, in patients who had an electrocardiographic pattern of "left ventricular strain".

Adult↗
PubMedDate not supplied

[The hemodialysed patient and his/her family caregiver. Comparison of perceptions of the chronic illness].

BACKGROUND: According to health psychology, the family caregiver (fc), i.e. the person who takes care of a hemodialysed patient, plays a pivotal role in coping with dialysis. This study explored and compared the lifestyle and the main needs of a cohort of hemodialysis patients, with reduced personal autonomy, to their fc, evaluating some psychological functional parameters, such as the perception of familial and social support, the psychological quality of life, the disability due to chronic illness, and the communication style. METHODS: An anonymous multiple versions questionnaire, administered according to the caregiver's family relationship, was given for self assessment to 54 couples of patients and related fc (spouse, son/daughter and brother/sister), mean age 66 and 60, respectively; mean dialytic patients' age: 8 years and 6 months. The questionnaire consisted of three different sections, demographics, renal disease and psychological evaluation, with 4 standard scales (Social Support Satisfaction, Marital Communication, Psychological General Well-Being Index and Evaluation of Needs). A multivariate variance analysis (MANOVA) was subsequently performed. RESULTS: Women have a higher perception of their lifestyle change after dialysis, and, in general, patients communicate more easily with their fc than vice versa. Communication problems are more common in patients with a recent diagnosis. Patients and fc mostly need a better dialogue with their nephrologists and urge some psychological help. CONCLUSIONS: The quality of the relationship between physicians, patients and their families is a key element in the process of healing.

Adult↗
PubMedDate not supplied

Flow cytometric study of immunocompetent cell phenotypes and phagocytosis in CAPD effluent.

The immunocompetent cells from peritoneal effluent in 22 stable CAPD patients were studied for surface phenotype distribution and for phagocytic properties by flow cytometry. The following monoclonal antibody couples were employed with direct dual color immunofluorescence: HLA-DR/CD 4, CD 8/CD 11c, Leu 7/CD 16, CD 14/CD 13, and Anti-Leucocyte/CD33. The phagocytic properties of peritoneal macrophages and polymorphs (PMN) were evaluated by the ingestion of 2.02 microns O fluoresceinated microspheres. The same analyses were also performed in peripheral blood. A FACStar laser-operated flow cytometer was employed. A remarkable heterogeneity of CAPD fluid cells was demonstrated, even in the absence of any obvious infection, suggesting the possible occurrence of aborted inflammatory processes. Activated T helper cells, T suppressors (CD8+/CD11+) and Fc gamma+ cells prevailed among lymphocytes, whereas variable amounts of monocytes and PMNs were present. The phagocytic activity of CAPD fluid phagocytes ranged from very depressed to normal values, whereas it was only moderately reduced in blood, suggesting local blocking phenomena, possibly due to interfering metabolites. Flow cytometry is the method of choice for a rapid and accurate analysis of phenotypic and functional properties of cells recovered from various body fluids. This study may be helpful in evaluating local immune response mechanisms in CAPD patients.

Adult↗
PubMedDate not supplied

Effect of n-3 polyunsaturated fatty acids on cyclosporine pharmacokinetics in kidney graft recipients: a randomized placebo-controlled study.

Highly concentrated marine polyunsaturated fatty acids (n-3 PUFA), affecting the lipids and lipophilic drugs metabolism, can interfere with cyclosporine (CyA) pharmacokinetics. This prospective, randomized and placebo-controlled, double-blind study involved 42 kidney graft recipients. From day +1, 21 pts (E) received 6 g n-3 PUFA (85% EPA + DHA, Esapent, Pharmacia) and 21 pts (P) received placebo (olive oil), both reduced to 3 g from day +30 on. A quadruple immunosuppressive regimen was employed. Plasma creatinine, lipids and CyA pharmacokinetics were investigated 1, 3, 6, 9 and 12 months after graft. The two groups were comparable for age, weight, M/F ratio, hypertension prevalence and baseline lipids. Active treatment did not affect total and HDL-cholesterol, but significantly lowered triglycerides (E:120 +/- 12 vs P:166 +/- 21 mg/dl, p < 0.0001). At one year, E pts had lower creatinine than P (1.26 +/- 0.06 vs. 1.88 +/- 0.2 mg/dl, p < 0.05), comparable CyA dosage, and a larger CyA area under the curve (AUC) (n.s.), with a higher blood peak level (Cmax) (p < 0.04) and less variance in time to peak (n.s.). The larger AUC in the E group at all intervals and the better pattern of plasma creatinine, with no rise in blood pressure, provided evidence of better CyA absorption and metabolism in n-3 PUFA supplemented kidney graft recipients.

Adult↗