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G Brun

Publications and source records attributed to G Brun.

161 records · Page 9Linked to original sources
PubMedDate not supplied

[A genetic study of the interaction of Piry virus with drosophila].

Piry virus mutants selected for altered growth properties in Drosophila melanogaster have previously been designated agD mutants. This paper presents a classification scheme for grouping some of these mutants and also presents the characteristics of some members of each group. Analysis, for each agD clone, of the mean incubation time at 20 degrees C relative to the mean incubation time at 28 degrees C (t20,t28), compared to the range of similar data for wild-type virus enabled us to define 4 classes of agD mutant: (1) mutants equally affected at both temperatures, (2) cold-sensitive mutants in drosophila (csD), (3) heat-sensitive mutants in drosophila (tsD) and (4) mutants whose mean incubation time could not be estimated at either 20 or 28 degrees C. All agD mutants are listed. The majority of them were also ts in chicken embryo cells (CEC), and some were classified into complementation groups. Some correlations were noted. For example, the agD mutants that were ts+ in CEC were frequently (7 out of 12) weakly cold-sensitive in drosophila. Those tsD mutants which were also ts in CEC, all belonged to complementation group I (L protein). True csD mutants, whose divergence of incubation time with wt regularly increased, and which were also ts in CEC, all belonged to group I except for one which belonged to group V (G protein). Three mutants fell into a distinct sub-class of csD mutants, characterized by a defect in the CO2 sensitivity symptom expression below a definite temperature. One of these which was further characterized was shown to be in complementation group V. Some mutants of the fourth class were examined for their fly-invading capacity. This was found to be more-or-less strongly affected. Among these mutants, independent of this phenomenon, mutations were assigned to L, N and G proteins, suggesting that different physiological defects are involved. A comparison was made of the phenotypes in drosophila of some of the agD mutants with other members of the vesiculovirus family. The analogy of sigma rhabdovirus with tsD mutants is also reported.

Animals↗
PubMedDate not supplied

Cytologic findings in a neovagina created with Vecchietti's technique for treating vaginal aplasia.

OBJECTIVE: To report long-term cytologic findings after treatment of congenital vaginal aplasia or Mayer-Rokitansky-Küster-Hauser syndrome (MRKH) using progressive traction of the vulvar tissues, or Vecchietti's technique (VT). STUDY DESIGN: In eight women with MRKH, neovaginal smears stained by the Harris-Schorr technique were collected 2-12 years after VT. RESULTS: Eosinophilic superficial cells observed in all cases indicate that the neovaginal epithelium responds to hormonal variations. The presence of Döderlein bacilli (seven cases) suggests that the regional environment of the neovagina is almost the same as that of the normal vagina. Gardnerella vaginalis (one case) and human papilloma virus infection (low grade squamous intraepithelial lesions) (one case) were also observed. CONCLUSION: Neovaginal smears taken after the cicatrization period following construction of a neovagina by VT show a remarkable resemblance to those from normal vaginal mucosa and suggest that these neovaginas are almost the same as normal vaginas.

Adolescent↗
PubMedDate not supplied

[Breast cytology. Its place in the diagnosis of tumors].

Our work consists in the review of the experience in our hospital over 9 months and 110 cases in which tumours of the breast were seen. In most of them a clinical study, a radiological study, a thermographic study and a cytological and histological study was carried out. This shows that an examination of the ctyology is a very useful addition to the work-up to be carried out before therapy is started. The difficulty in cytology of the breast lies in the obviously serious problems in interpretation of the appearances, and explains why there are 2.7% of false negatives and 0.9% of false positives. All the same we think that we can find out how to lessen the number of these failures in diagnosis by a better knowledge of the traps to be found in breast cytology. Far from being a substitute for pathological anatomy, cytology is an indispensable complement to the study of the pathology of the breast.

Biopsy, Needle↗
PubMedDate not supplied

Interstrain comparison of hepatic and renal microsomal carcinogen metabolism and liver S9-mediated mutagenicity in DA and Lewis rats phenotyped as poor and extensive metabolizers of debrisoquine.

Lewis (extensive metabolizers) and DA (poor metabolizers) rat strains show genetic polymorphism for the gene regulating debrisoquine 4-hydroxylation in a manner analogous to human drug polymorphism and show also a different toxicological response to aflatoxin B1. In order to investigate the underlying mechanism(s) of the different drug-metabolizing capacities, the in vitro metabolism and liver S9-mediated mutagenicity of several drugs and carcinogens were studied in female Lewis and DA rat strains, using untreated, 3-methylcholanthrene-treated, and phenobarbital-treated animals. S9-mediated mutagenicity of aflatoxin B1 and hepatic and renal ochratoxin A 4-hydroxylase activity were much lower in DA rats; however, the activity of a number of other hepatic and renal drug-metabolizing enzymes did not show any interstrain difference. Slight strain differences were found in the inducibility of several hepatic drug-metabolizing enzymes, except for ochratoxin A 4-hydroxylase activity; the latter was inducible only by 3-methylcholanthrene in the DA strain and appeared to be linked genetically with other Ah locus-associated monooxygenases. Our data suggest that ochratoxin A 4-hydroxylase activity, which is low in the DA strain, is catalyzed by a cytochrome P-450 isozyme different from that responsible for debrisoquine 4-hydroxylation. Our results provide some insight into why the two metabolic oxidation phenotypes show different susceptibility to cancer induction and to the toxicity of certain environmental carcinogens, such as aflatoxin B1 and ochratoxin A.

2-Acetylaminofluorene↗
PubMedDate not supplied

[Urologic consequences of severe genital prolapse].

From three observations, the authors analyse the ureteric dilatation and genital prolapse association from an anatomo-clinic point of view. The bladder-base displacement situated in the vaginal prolapse and the resulting very sharp angle formed by the terminal ureter portion are enough to explain the pathogenesis of the observed urinary lesions. The repercussions of advanced prolapses upon upper urinary tracts are rarely mentioned in literature. The three reported clinic cases are absolutely demonstrative: for every one, the major uretero pyelectasis is associated with a progressive renal insufficiency. Authors insist upon urologic symptoms quick regression after a prolapse cure. This correction is made either by putting a pessary, either by a surgical cure unless the risk should be too important for the patient.

Aged↗
PubMedDate not supplied

[Contraception and endometriosis].

Contraception in women with endometriosis is unusual. In case of mild asymptomatic endometriosis all contraceptive methods may be used except predominant estrogenic action pills. In case of severe endometriosis if score is upon 70 or adhesion score upon 50 the patients are generally infertile. Contraception is not necessary. If scores are lower, medical treatment of endometriosis insure the contraception during the first six months. Afterwards an hydroxyprogesterone derivative pill such as cyproterone acetate, or a norpregnan derivative pill, or a combined estrogen progestogen pill, with predominant progestogen action (norgestrienon), or a monophasic pill containing norethisteron, or in case of metabolic diseases a pill containing gestoden, must be prescribed three out of four weeks. In case of adenomyosis, vaginal contraception is better. Perhaps in the next future, the new progestogen sterilant will be the best.

Contraception↗