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Biomedical subjects

G Brice

Publications and source records attributed to G Brice.

25 records · Page 2Linked to original sources
PubMed1996

Patterns of ethanol consumption in a continuous access situation: the effect of adding a sweetener to the ethanol solution.

The effect of the addition of sucrose to an ethanol solution upon daily intake patterns was examined in a continuous-access operant situation with Wistar rats. Rats were first initiated to self-administer orally a 10% ethanol (v/v) solution using the sucrose-substitution procedure in 30-min limited-access conditions. When then studied in a continuous-access operant situation (23 hr ethanol access), substantial increases in ethanol consumption were found when varying concentrations of sucrose were added to the ethanol solutions presented. This increased consumption was found to be a complex function of both an increase in the number of drinking occurrences each day and in the size of each drinking occurrence. When 2% sucrose was compared with 2% sucrose/10% ethanol, the consumption of the sweetened ethanol was greater than consumption of the sweetener alone, suggesting that the ethanol added to the ability of the solution to maintain behavior beyond that of the sucrose alone. This study supports the use of sweetened ethanol solutions for the study of ethanol drinking patterns, and as a model system for examining factors involved in the regulation of ethanol consumption.

Alcohol Drinking↗
PubMed1994

Changes in satellite cell mitotic activity during acute period of unilateral diaphragm denervation.

The acute period of unilateral diaphragm denervation (DNV) is associated with increases in cell mitotic activity, protein synthesis, and muscle fiber hypertrophy. Our purpose was to determine whether acute unilateral diaphragm DNV is associated with changes in muscle isometric contractile properties, cross-sectional area of different muscle fiber types, mitotic activity of muscle fiber satellite cells, and muscle fiber ultrastructural properties indicative of injury. Adult male Fischer 344 rats underwent a right phrenicotomy, and DNV and intact (INT) hemidiaphragms were studied 72 h later. DNV hemidiaphragm displayed a significant decline in maximal isometric force (8.7 vs. 24.3 N/cm2) and a prolonged time to peak twitch force (47.8 vs. 37.5 ms) and time to half relaxation (72.3 vs. 44.3 ms) compared with INT contralateral hemidiaphragm (P < 0.05). DNV resulted in a significant increase in cross-sectional area of types I (33%), IIa (35%), and IIb (28%) fibers relative to INT hemidiaphragm (P < 0.05). Satellite cell mitotic activity (assessed by incorporation of bromodeoxyuridine) was approximately 5.5 times greater in DNV than in INT muscle (DNV 25.0 +/- 3.8, INT 4.5 +/- 1.4 labeled satellite cell nuclei/1,000 nuclei; P < 0.05). Ultrastructural examination of electron micrographs revealed alterations in Z-line and sarcomeric structure indicative of muscle injury. Cellular infiltration and segmental necrosis were also noted in some fibers. We conclude that acute unilateral diaphragm DNV results in muscle fiber injury that induces satellite cell activation. We also speculate that the specific force decrement associated with DNV is at least partially the result of muscle injury.

Animals↗
PubMed1986

Exercise tolerance and compensatory sympathetic tone during beta-blockade after myocardial infarction.

Using chronically instrumented dogs with a healed anterior wall myocardial infarction, we have assessed the effect of acute beta-adrenergic blockade on exercise tolerance, left ventricular (LV) performance, and myocardial VO2. Mongrel dogs were instrumented with Doppler flow probes around the left circumflex coronary artery, LV pressure cells, epicardial ECG electrodes, and left atrial and coronary sinus catheters. Myocardial infarction was produced by ligation of the left anterior descending coronary artery at time of instrumentation (approximately 20% LV mass). After a 1-month recovery period, dogs were subjected to two identical submaximal graded exercise tests on a treadmill on separate days, once without (control) and once with (propranolol, 1 mg X kg-1, i.v.) acute beta-adrenergic blockade. Heart rate, LV pressure, dP/dt max, left circumflex blood flow velocity, and myocardial VO2 index were measured at each stage of the graded exercise test. All variables were significantly reduced from control during beta-block in exercise. beta-Adrenergic blockade in the presence of a 1-month-old anterior wall myocardial infarction did not compromise exercise capacity during submaximal exercise.

Adrenergic beta-Antagonists↗
PubMedDate not supplied

Anti-PMSG antibody levels in sheep subjected annually to oestrus synchronisation.

Estimation of the long-term consequences on reproduction performance of the oestrus synchronisation treatments that are annually applied to ewes was carried out on nine officially controlled dairy flocks in the Roquefort region of France. A hormonal treatment combining the insertion of a vaginal fluoro-gestone acetate (FGA) sponge for 14 days and the injection of about 500 IU of pregnant mare serum gonadotropin (PMSG) at withdrawal was applied to the ewes in seven of the nine flocks. The ewes in the two other flocks were used as controls. Blood samples were taken from each female just before the treatment (to test for the presence of residual antibodies) and 20 days after the PMSG injection. Anti-PMSG antibody binding rates were calculated for each blood sample. The residual binding rate increased with age and induce negative effects on the following years reproduction performances, i.e., they increased the probability that the ewes would not become pregnant.

Analysis of Variance↗
PubMedDate not supplied

Acute responses to using walking poles in patients with coronary artery disease.

PURPOSE: This study was designed to assess the safety and effectiveness of walking when using 1-lb walking poles in Phase III/IV cardiac rehabilitation patients. METHODS: Following instruction on the proper use of the poles and adequate time to practice, each subject completed two 8-minute walking trials on a level treadmill either with or without walking poles. Each trial was conducted at an identical speed for each subject in a randomized order. Heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), and ratings of perceived exertion (RPE) were recorded every 2 minutes during each trial, while ECG responses (ST segment changes and dysrhythmias) were monitored continuously. RESULTS: Walking with poles significantly (P < .05) increased the energy cost of walking by 21% (3.8 mL/kg/min) compared to walking without poles. There were also significant (P < .05) increases in HR (14 bpm), SBP (16 mm Hg), and DBP (4 mm Hg) when comparing conditions. Calculated oxygen pulse (mL O2.heart beat) values indicated that changes in HR were consistent with the increase in VO2 and were not related to a pressor response mechanism. The only dysrhythmias noted were isolated PVCs, with no differences in the frequency of occurrence between trials. There were no adverse ST segment changes with either trial. CONCLUSIONS: These data demonstrate that at a given speed, the use of 1-lb walking poles can safely increase the intensity of walking exercise in Phase III/IV cardiac rehabilitation patients.

Aged↗
PubMedDate not supplied

Validity of body composition assessment methods for older men with cardiac disease.

PURPOSE: This study was designed to determine which of several body composition assessment methods was most accurate for patients with cardiac disease for the purpose of outcome measurement. METHODS: Six body composition assessment methods were administered to each of 24 men with cardiac disease. Methods included circumference measurement, skinfold measurement, near-infrared interactance via the Futrex-5000, bioelectrical impedance via the BioAnalogics ElectroLipoGraph and Tanita TBF-150, and hydrostatic weighing, the criterion measure. RESULTS: A repeated measures analysis of variance indicated no significant (P > .05) difference between circumference and skinfold measurements compared to hydrostatic weighing. Near-infrared interactance presented the best standard error of estimates (3.5%) and the best correlation (r = .84) with hydrostatic weighing; however, the constant error was 3.76%. Bioelectrical impedance measured by the ElectroLipoGraph and TBF-150 instruments significantly underestimated percent body fat by 8.81% and 4.8%, respectively. CONCLUSIONS: In this study of middle-aged to older men with cardiac disease, the best method for determining body fat was circumferences. This technique was accurate, easy to administer, inexpensive, and had a lower error potential than the other techniques. Skinfold measurements were also closely related to hydrostatic weighing, but should be performed only by experienced practitioners because there is a greater potential for tester error in certain patients. In the future, near-infrared interactance measurements may be a viable technique for body composition assessment in patients with cardiac disease. However, algorithms specific to the population of patients with cardiac disease being tested must be developed before this technique can be routinely recommended for body composition assessment. Bioelectrical impedance assessment by either method is not recommended for patients with cardiac disease, as it consistently underestimated percent body fat when compared to hydrostatic weighing in this population.

Adipose Tissue↗