Search PubMed⌕ Search

Biomedical subjects

G Brasseur

Publications and source records attributed to G Brasseur.

At least 73 records · Page 4Linked to original sources
PubMed1994

Non-native intragenic reversions selected from Saccharomyces cerevisiae cytochrome b-deficient mutants. Structural and functional features of the catalytic center N domain.

A total of 110 revertants have been isolated from two well-characterized cytochrome b deficient (mit-) mutants. The mit- mutations are located in an extramembranous loop linking the transmembrane alpha-helices IV and V of cytochrome b which has been postulated to be part of the catalytic center QN and therefore is assumed to be essential for the functioning of the bc1 complex. The molecular bases of the reversions were identified by sequencing the cytochrome b mRNAs. This allowed us to identify seven new structures of cytochrome b which are more or less compatible with its catalytic activity. The secondary mutations occurred either at the level of the original site mutation or at adjacent positions (region 204-208 of the polypeptide chain), or even at a distance of more than 150 amino acids (position 30) suggesting topological interaction between these two areas. All the revertants recovered cytochrome contents and phosphorylation efficiencies similar to the wild-type ones, albeit differences appeared in their specific growth rates and NADH respirations. The failure in bc1 complex functioning induced by the mutation S206L and its restoration by non native reversions are tentatively explained.

Amino Acid Sequence↗
PubMed1994

Successful treatment of Acanthamoeba keratitis by hexamidine.

Acanthamoeba keratitis remains very difficult to treat because of the lack of antiamoebic agents completely effective against cysts. Currently, the recommended treatment includes the use of topical neomycin sulfate, various imidazoles, and propamidine isethionate (Brolene) 0.1% eye drops, a compound of the diamidine family. In the present article, we describe the successful management of two patients with an Acanthamoeba keratitis, treated with hexamidine diisethionate (Desomedine) 0.1% eye drops, another diamidine derivative, which was found amoebicidal in vitro on the isolated Acanthamoeba strains. This is to our knowledge the first report on the amoebicidal effectiveness of hexamidine, simultaneously in vitro and in vivo.

Acanthamoeba↗
PubMed1993

[Ultrasonographic study of posterior vitreous detachment in emmetropic eyes].

Vitreoretinal relationships can be evaluated by biomicroscopic examination and ultrasound. We analyzed 220 normal and emmetropic eyes in a population between the ages of 20 to 80 years to evaluate the position of the posterior vitreous surface (prospective study). We found that: Most cases of very partial and very peripheral P.V.D. (71/220 eyes; 32%), especially those confined to the nasal quadrant (50/71 eyes), corresponded to a simple increase of reflectivity of the vitreous base rather to than real PVD. Twenty-five per cent of the patients over the age of 80 years did not have PVD. The frequency of partial PVD located in 2, 3 and 4 quadrants except the posterior pole was very low, regardless of age. These partial PVD are transient. Fifty-eight per cent of the patients over the age of 80 years had a complete PVD. This percentage is clearly lower than that reported by most of the large biomicroscopic studies. The extension of PVD always begins at the vitreous base and ends at the posterior pole. This extension occurs around the age of 60-65 years and results, in most cases (35/38 eyes; 92%), in complete PVD with collapse. A prepapillary ring is a specific sign of complete PVD with or without collapse, but its absence (5/38 eyes) does not indicate the absence of PVD. The frequency of giant lacune is maximal (16/32 eyes; 50%) for young people between the ages of 30 and 40 years, then decreases as PVD becomes complete. The average level of reflectivity of the vitreous body is highest for patients between the ages of 70 and 80 years, but this variation is only very slight. This study also stresses the clinical criteria used to make the ultrasonic differential diagnosis between PVD and giant lacunae.

Adult↗
PubMed1991

[Treatment of dry eye syndrome with lacrimal gel: a randomized multicenter study].

The appearance on the French market of a carbomer tear substitute, Gel-Larmes, an original galenic form, constitutes a real therapeutic progress in the treatment of dry eye syndrome. An open randomized multicentric trial was conducted comparing the tolerance and efficacy of Gel-Larmes with those of eye drops containing chondroitin sulphate. The statistical analysis evidences an inferior average number of daily instillations for Gel-Larmes, its better efficacy on the symptoms as well as on the objective criteria: biomicroscopic examination, break-up time, rose Bengal test. The tolerance of the product is excellent and 81% of the patients express their wish to pursue Gel-Larmes versus 54% for the reference product.

Acrylic Resins↗
PubMed1991

[Retinal toxicity caused by intravitreous injection of an aminoglycoside. Apropos of a case].

The authors present a case of severe retinal ischemia associated with Dibekacin intraocular injection. This aminoglycoside was used, at an inadvertent dose, for the treatment of a postoperative endophthalmitis. The prominent finding at ocular ophthalmoscopy was oedematous retina with hemorrhages in the macular area. Fluorescein angiography revealed severe retinal vascular non perfusion. The eye subsequently developed neovascular glaucoma. Precautions are necessary to prevent such a catastrophic event; the injectable solution must be prepared on the surgical field, the injection should be performed slowly in the anterior vitreous without exceeding 0.2 mg when Gentamicin is used.

Aged↗
PubMed1990

[Retinal photocoagulation without corneal contact using the 90 diopter lens].

The authors used the hand held 90 diopter lens for practice of non contact retinal photocoagulation in 8 cases when ocular conditions make undesirable the use of a contact lens. The excellent visualisation of the retina, patients comfort, his utilisation immediately after surgery and in gaz filled, are among its more interesting uses. The movements of the eye and the impossibility to go further than the mid periphery of the retina are its principals disadvantages.

Adult↗
PubMed1989

[Biomicroscopy of the fundus oculi using a 90 diopter lens].

An improved method of indirect biomicroscopic examination of the ocular fundus has been made possible with the use of a + 90 D lens since 1985. Its special optical characteristics make it a very useful instrument. During this study of 220 retinal detachments we compared this instrument with other methods for examining the ocular fundus (Goldmann fundus contact lens, panfundoscope, binocular indirect ophthalmoscope with a 20 D lens). Other uses for the lens are described. This non-contact examining technique provides excellent visualisation, a wide field of vision, and good patient tolerance. The 90 D lens is particularly useful in vitreo-retinal management of retinal detachment both during and immediately after surgery.

Humans↗
PubMed1989

[Endophthalmitis caused by Aspergillus (anatomo-clinical study of a case)].

The authors report a case of endogenous aspergillosis endophthalmitis. Ocular involvement was very important and not controlled by several vitrectomy and intra-vitreous Amphotericin B. Pathological examination revealed abscesses in the vitreous, choroid and retina. Diagnosis and treatment are discussed.

Aspergillosis↗
PubMed1989

[Listeria endophthalmitis: an uncommon etiology].

Listeria monocytogenes is an uncommon cause infections: to our knowledge this is the seventh case of human endophthalmitis isoleted. An early diagnosis and therapy allowed an excellent functional recovery when compared to the reported cases of the literature.

Anti-Bacterial Agents↗