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Biomedical subjects

G Bradford

Publications and source records attributed to G Bradford.

7 recordsLinked to original sources
PubMed1999

Workforce wonder.

Explore the source record for details and available documents.

Efficiency, Organizational↗
PubMed1993

Temporal thrombocytopenia after engraftment with defined stem cells with long-term marrow reconstituting activity.

Delayed platelet recovery and altered marrow megakaryocytopoiesis was observed in highly irradiated mice following transplantation with 1000 lineage-negative, stem cell antigen-positive (Lin-Sca-1+) cells. Thirty days after transplantation, the mice were thrombocytopenic. Normal platelet levels were reestablished by 90 days after transplantation. Platelet levels were established from the bone marrow with altered megakaryocytopoiesis, however. Megakaryocyte progenitor numbers were found at reduced levels in the reconstituted marrow at all time points assessed. Bone marrow function was also different in that marrow reserve was also diminished. While the transplantation regime did give long-term reconstitution of blood cells, the quality of the marrow reserve was significantly impaired as revealed by response to further hematopoietic challenge. The data indicate that mice transplanted with a population of highly defined stem cells have perturbed marrow function, one characteristic being an altered process of megakaryocytopoiesis.

Animals↗
PubMed1992

Immunohistologic demonstration of myocardial proteins with applications.

The authors used antibodies specific for creatine kinase MB isoenzyme (CK-MB) and myosin light chain-1 (MLC-1) for immunohistologic staining. At appropriate dilutions of antibody, frozen sections of human heart muscle were positive for both CK-MB and MLC-1, whereas sections of human skeletal muscle were negative for both proteins. Staining for both CK-MB and MLC-1 also was demonstrated in an immature teratoma. Furthermore, staining was localized to the rhabdomyosarcomatous elements within the teratoma; other components of the tumor did not stain for CK-MB or MLC-1. Biopsies of skeletal muscle revealed that regenerative, but not intact normal or degenerating, fibers also contained CK-MB and MLC-1. Immunohistologic stains for CK-MB and MLC-1 may be useful as tumor markers and as markers for regenerative muscle fibers.

Biopsy↗
PubMed1988

Secretion of creatine kinase MB isoenzyme by an immature teratoma with predominant rhabdomyosarcomatous elements.

A 37-year-old man with metastatic immature (malignant) teratoma with prominent rhabdomyosarcomatous elements had markedly increased activity of creatine kinase (EC 2.7.3.2) MB in serum. There was no electrocardiographic evidence of infarction or ischemia, and autopsy revealed no myocardial infarction, significant coronary atherosclerosis, myocarditis, or invasion of the heart by tumor. A high proportion of the creatine kinase activity in a homogenate of the tumor was attributable to the MB isoenzyme. Persistent increases of creatine kinase-MB and an unusually high MB isoenzyme activity, out of proportion to total creatine kinase activity, may indicate a nonmyocardial origin of this isoenzyme.

Adult↗