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Biomedical subjects

G Bonora

Publications and source records attributed to G Bonora.

At least 37 records · Page 2Linked to original sources
PubMed1984

Effect of secretin stimulation upon serum immunoreactive trypsin in patients with carcinoma of the pancreas.

The response of serum immunoreactive trypsin (IRT) to the intravenous injection of secretin (75 clinical units) was evaluated in 14 patients with carcinoma of the pancreas and in 20 healthy control patients. A striking increase of serum IRT concentration after secretin, which was much more marked than in those in the control group, was found in patients with localized carcinoma of the pancreas, whereas no increase was observed in those with more diffuse carcinoma.

Adult↗
PubMed1983

Comparative study of serum pancreatic isoamylase, lipase, and trypsin-like immunoreactivity in pancreatic disease.

Serum total amylase, pancreatic and salivary isoamylase, lipase and trypsin-like immunoreactivity (TLI) were measured in 16 patients with acute pancreatitis, 37 patients with chronic pancreatitis, 11 patients with pancreatic cancer, and 53 control subjects in order to evaluate the relative value of these tests in the diagnosis of pancreatic disease. In acute pancreatitis patients studied within 2 days from the onset of pain all pancreatic enzymes were abnormally high. In chronic pancreatitis patients serum pancreatic isoamylase and TLI were abnormally low in 8 out of 10 patients with severely impaired pancreatic exocrine function, while lipase was abnormally low in 6 patients. During acute exacerbations of the disease elevated levels of pancreatic isoamylase and lipase, but not of TLI, were found in about one third of cases. In patients with pancreatic cancer the pattern of changes in serum pancreatic enzymes was variable since levels within, below and above the normal range were found. The results demonstrate that in acute pancreatitis all serum pancreatic enzymes had the same diagnostic sensitivity, however serum lipase determination is the most convenient because of its simplicity and low cost. In chronic pancreatitis serum pancreatic isoamylase and TLI may be useful in detecting severe pancreatic insufficiency. In pancreatic cancer serum pancreatic enzymes lack diagnostic specificity.

Adult↗
PubMed1983

Medical treatment of Zollinger-Ellison syndrome with ranitidine.

Ten patients with Zollinger-Ellison syndrome (4 with intact stomach and 6 with previous partial gastrectomy) received ranitidine (600-1200 mg daily) for 2-24 months (mean 14 months). The inhibitory effect of ranitidine on acid secretion was evaluated by measuring acid output during the third hour following a single drug administration in all cases, and by the 24-hour intragastric pH profile in 5 patients. Therapeutic efficacy was assessed by clinical and endoscopic criteria. Ranitidine at different dosages, related to the rate of acid secretion, was effective in controlling peptic disease and diarrhoea in the majority of patients. The secretory parameters used in this study allowed evaluation of the inhibitory effect of ranitidine on acid secretion, in order to select the best therapeutic dose and to decide on surgical cure in the patients non-responsive even to the highest dosage.

Adolescent↗
PubMed1983

Effect of bombesin on serum immunoreactive trypsin in healthy subjects and in patients with chronic pancreatitis.

We studied the effect of bombesin (9 ng/kg X min for 30 min by intravenous infusion) on serum immunoreactive trypsin in healthy subjects and in chronic pancreatitis patients. Bombesin administration caused a marked and significant increase of serum immunoreactive trypsin concentration in healthy subjects. The increase occurred in the first 15 min after the beginning of bombesin infusion and persisted for the duration of the study (2 h). In patients with chronic pancreatitis, the increase was much less pronounced. In these patients, the integrated immunoreactive trypsin response to bombesin was significantly correlated with bicarbonate, lipase, and chymotrypsin outputs into the duodenum. The response of serum immunoreactive trypsin to bombesin stimulation seems to vary according to the degree of pancreatic exocrine dysfunction and to reflect the functional capacity of acinar cell mass.

Adult↗
PubMed1981

Secretin-stimulated trypsin-like immunoreactivity in alcoholics.

Serum trypsin-like immunoreactivity (TLI) was studied in alcoholics without evidence of pancreatic disease and in controls. Basal values were 29 +/- 4.6 microgram/l (mean +/- S.E.M) in alcoholics and 23 +/- 4.4 microgram/l in controls (p not significant). The injection of secretin was followed by a significant increase of serum TLI in both groups; the integrated trypsin output (ITO) in the first hour after secretin administration was 947 +/- 403 (mean +/- S.E.M.) in alcoholics and 76 +/- 15 in controls (p less than 0.05). In 9 (75%) of the alcoholics tested, ITO was higher than the highest ITO of controls. The increase of serum TLI after injection of secretin is probably due to secretion and/or regurgitation of trypsinogen into the bloodstream when the pancreas is stimulated with intravenous secretin. In the light of experimental studies on chronic ethanol intoxication in animals, the increased ITO observed in alcoholics may suggest obstruction to pancreatic secretory flow in spite of the absence of any clinical sign of pancreatic disease.

Adult↗
PubMed1980

Serum trypsin-like immunoreactivity and secondary hyperparathyroidism in renal failure.

UNLABELLED: In 46 kidney patients aged from 30 to 71 years, after an overnight fast, serum levels of trypsin (TLI), gastrin (Gas), parathromone (PTH), calcium (Ca), magnesium (Mg), phosphate (iP), amylase, were evaluated. Ten patients had a normal renal function; in 18 patients glomerular filtration rate (GFR) ranged from 5 to 70 ml/m'; 18 patients with GFR below 2 ml/m' were treated with 4 hours hemodialysis. In dialysed patients TLI was evaluated before and after dialysis. In patients with FGR ranging from 120 to 5 ml/m' a significant inverse correlation (p < 0.001) appeared between TLI and GFR values. In the same group a significant direct correlation (p < 0.001) was found between PTH and TLI. The study showed also a significant direct correlation between amylase and TLI (p < 0.001); likewise TLI, amylase was inversely correlated to GFR (p < 0.01) and directly to PTH (p < 0.001). No correlation was found between TLI and Gas, Ca, Mg, iP. A significant inverse correlation was found between PTH and GFR (p < 0.01). Because serum level of PTH, amylase and TLI were related to GFR values, we calculated partial correlation between these parameters. The partial correlation coefficients of the relation between PTH and TLI, PTH amylase, holding GFR constant, were higly significant (p < 0.001). On the contrary, holding a constant PTH, the correlations between pancreatic enzymes and GFR were not confirmed. Pre-dialysis TLI levels were significantly higher than post-dialysis (p < 0.05). IN CONCLUSION: 1) TLI and amylase have the same behaviour in renal failure; 2) the levels of both enzymes are high in renal insufficiency; their values seem to be correlated to secondary byperparathyroidism.

Adult↗
PubMed1980

Serum trypsin-like immunoreactivity after endoscopic retrograde cholangiopancreatography.

Serum trypsin-like immunoreactivity (TLI) was studied in 31 patients before and after endoscopic retrograde cholangiopancreatography. None of the patients developed clinical acute pancreatitis. Generally, the serum TLI peak was observed within the first 6 hours after the examination. Most patients (73%) showed a pathologically high TLI but very high values were not frequent (19%). Successful pancreatic opacification was followed by a significant increase in serum TLI which was pathologically high in nearly all cases (18/20). On the contrary, after cholangiography alone abnormal values were less frequent (4/8) and the increase was not significant. In most patients TLI and amylase responses were in agreement. A significant, though poor, linear relation was found between serum TLI and serum amylase 3, 6 and 12 hours after the examination.

Amylases↗
PubMed1980

Basal and stimulated serum immunoreactive trypsin in normal subjects.

Fasting serum immunoreactive trypsin (IRT) concentrations were measured in 35 healthy subjects. In 10 of them serum IRT levels were also evaluated after administration of secretin (GIH, 75 CU i.v. over 2 minutes); in 7 after a Lundh meal; in 12 after a standard protein meal (Liebig meat extract, 15 g in 150 ml of water) and in 13 after a glucose (1 g/Kg) oral load. Serum IRT was measured by radioimmunoassay (Trypsik kit, CIS, Sorin-Biomedica, Saluggia, Vercelli, Italy). Fasting serum IRT was 25.8 +/- 9 ng/ml (mean +/- SD). No correlation was found between age and serum IRT levels, nor were any statistical differrences observed in IRT concentration between the two sexes. A standard protein meal, Lundh meal or oral load of glucose did not provoke a significant increase in serum IRT levels. Only rapid i.v. injection of secretin was able to induce a significant rise of IRT over basal levels in healthy subjects. This behaviour is probably due more to an engorgement of the pancreatic ductal system with regurgitation of trypsinogen into the blood steam rather than to a direct effect of secretin on the synthesis and secretion of trypsin or to intestinal reabsorption of this enzyme.

Adolescent↗
PubMed1980

Serum immunoreactive trypsin after secretin stimulation in chronic pancreatitis.

Serum immunoreactive trypsin (IRT) response to secretin injection was studied in 13 patients with chronic pancreatitis with different degrees of exocrine dysfunction and in 10 control subjects. The maximal increase of serum IRT from basal values and the integrated trypsin output (ITO) after secretin administration were significantly correlated with the output of chymotrypsin into the duodenum during caerulein-secretin infusion (p < 0.01), but not with the output of lipase nor of bicarbonate. Serum IRT response to secretin stimulation was greater in 4 of the 5 patients with chronic pancreatitis with mild to moderate exocrine dysfunction than in the control group, suggesting an increased regurgitation of IRT into the blood stream by the pancreas, probably due to some degree of obstruction to pancreatic secretory flow in absence of severe acinar cell damage. Conversely, the response of serum IRT after secretin administration in 7 of the 8 patients with severe exocrine pancreatic deficiency was lower than in control subjects, probably because of the advanced distruction of the acinar pancreatic tissue. The response of serum IRT to secretin stimulation seems to vary following pancreatic function impairment and might reflect the degree of pancreatic exocrine dysfunction in chronic pancreatitis.

Adolescent↗
PubMed1980

Serum immunoreactive trypsin in type IV hyperlipoproteinemia.

Serum immunoreactive trypsin (IRT) under basal conditions and after pancreatic stimulation with secretin was studied in 10 patients with type IV hyperlipoproteinemia (HLP) and in 10 control subjects. No significant difference was observed between basal values of the two groups (p = NS). The increase of serum IRT was already significant 5 minutes after secretin administration (p < 0.01) and persisted with significance for one hour in both groups. The integrated trypsin output (ITO) was significantly greater in type IV HLP than in controls (510.3 +/- 17.8 and 72.2 +/ 17.6 respectively, mean +/- SEM, p < 0.0125). Only 2 (20%) of 10 patients with HLP had an ITO in the range of the controls. No significant correlation was found between ITO and triglyceride levels (p = NS). The response of serum IRT to secretin in HLP patients appears comparable to that observed in alcoholics and in patients with chronic pancreatitis with mild to moderate exocrine dysfunction, in whom there may be an abnormal regurgitation of trypsin-like material into the blood stream after secretin stimulation, probably due to an obstruction to pancreatic secretory flow. A similar obstructive mechanism may be hypothesized also in patients with HLP, but no data concerning the exocrine pancreatic secretion and the histological features of the pancreas are available to confirm this hypothesis.

Adult↗