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Biomedical subjects

G Bogosian

Publications and source records attributed to G Bogosian.

23 records · Page 2Linked to original sources
PubMed1983

Studies on the interaction of Trp holorepressor with several operators. Evidence that the target need not be palindromic.

The interaction of Trp repressor protein with partial trp operators was studied in vitro and in vivo. At high ratios of protein to DNA, Trp holorepressor formed stable complexes with DNA molecules containing half operators. When plasmids conferring the capacity to hyperproduce Trp repressor were present in trpOc strains of Escherichia coli, repression of downstream tryptophan synthase occurred. Palindromicity of the trp operator may facilitate stable interaction with Trp repressor, but this attribute need not be regarded as a critically essential structural feature. Sufficient information for the recognition by Trp repressor protein of an appropriate target resides within a DNA sequence of approximately ten base-pairs.

Bacterial Proteins↗
PubMed1983

Trp repressor protein is capable of intruding into other amino acid biosynthetic systems.

Escherichia coli strains with elevated intracellular levels of Trp repressor protein displayed complete growth inhibition on minimal media which contained high levels of tryptophan. The inhibition was attributable to the acquisition of a compound nutritional requirement, which could be satisfied by a combination of isoleucine, leucine, valine, threonine, serine, phenylalanine, and tyrosine. It is proposed that Trp repressor protein, at elevated levels, represses the transcription of those genes which encode enzymes for the biosynthesis of these particular amino acids. Data which support this model are presented, together with a discussion of its regulatory implications.

Amino Acids↗
PubMed1983

Indolmycin-mediated inhibition and stimulation of transcription at the trp promoter of Escherichia coli.

Escherichia coli cells harboring a non-attenuated trp-lac operon fusion were used to evaluate the effects of indolmycin on the initiation of transcription at the trp promoter. Indolmycin caused repression in trpR+ strains and in trpR deletion mutants, although higher effector concentrations were required in the latter situation. Plasmid-mediated elevation in tryptophanyl-tRNA synthetase reversed the inhibitory effect of indolmycin. Indolmycin did not facilitate the binding of purified Trp repressor protein to trp operator DNA.

Bacterial Proteins↗
PubMed1980

DNA sequence of the E. coli trpR gene and prediction of the amino acid sequence of Trp repressor.

A DNA sequence of 1041 base pairs from a BamHI fragment containing the E. coli trpR gene has been determined. With this sequence and other experimental evidence, the primary structure (88 amino acids) of the Trp repressor can be predicted. Additional features of the DNA sequences include a 22 base pair region upstream from the proposed structural gene which exhibits striking homology with the trp operator, thus implying that expression of the trpR gene may be under autogenous regulation.

Amino Acid Sequence↗