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Biomedical subjects

G Boccardo

Publications and source records attributed to G Boccardo.

35 records · Page 2Linked to original sources
PubMed1988

In vitro synthesis of double-stranded RNA by carnation cryptic virus-associated RNA-dependent RNA polymerase.

Partially purified carnation cryptic virus (CarCV) preparations possessed RNA-dependent RNA polymerase activity which was absent in comparable preparations from virus-free carnations. Enzyme activity was dependent upon the presence of virus particles, Mg2+, and the four ribonucleoside triphosphates, and was insensitive to inhibitors of DNA-dependent RNA polymerases. The 32P-labeled enzyme reaction products were largely dsRNAs as indicated by resistance to S1 nuclease and RNase A at high but not low ionic strength. The in vitro synthesized dsRNAs hybridized specifically with CarCV genomic dsRNAs, and the radioactive products present in the polymerase reaction mixture sedimented with the virus particles in sucrose density gradients. The data suggest that the RNA-dependent RNA polymerase associated with CarCV particles is a replicase which catalyzes the synthesis of copies of the genomic dsRNAs.

Plant Viruses↗
PubMed1987

Cryptic viruses in hop trefoil (Medicago lupulina) and their relationships to other cryptic viruses in legumes.

Three double-stranded (ds) RNA-containing viruses, named hop trefoil cryptic virus (HTCV) 1, 2 and 3, were found in apparently healthy seedlings of hop trefoil (Medicago lupulina). Only 1-2% of plants tested were virus-free. The particles occurred in the cytoplasm, nuclei and nucleoli of parenchyma cells. They were purified as a mixture (HTCV-M) with a mean yield of 50-100 micrograms/kg fresh tissue, and an antiserum was obtained. HTCV1 was serologically related to one component (ACV1) of a mixture of alfalfa cryptic viruses (ACV-M) present in alfalfa (M. sativa), and was separated by immunoprecipitation from HTCV-M; its particles, about 30 nm in diameter, contained two dsRNAs (Mr 1.27 X 10(6) and 1.15 X 10(6)) comigrating in PAGE with two RNAs of ACV-M. Particles of HTCV2, about 38 nm in diameter and with distinct morphological subunits, were serologically closely related to particles of similar morphology (RCCV2) present in a mixture of cryptic viruses (RCCV-M) from red clover (Trifolium pratense), and were distantly related to white clover cryptic virus 2, also of similar morphology. HTCV3 had particles 30 nm in diameter, morphologically similar to those of HTCV1, and reacted with an antiserum to vicia cryptic virus. HTCV2 and 3 could not be individually separated. The RNAs of HTCV-M, in addition to the two bands attributed to HTCV1, gave, upon electrophoresis, three bands of estimated Mr 1.34, 1.08 and 0.95, all X 10(6), and up to five minor bands of Mr ranging from 1.45 to 0.98, all X 10(6).

Fabaceae↗
PubMed1986

Relationships between the cryptic and temperate viruses of alfalfa, beet and white clover.

Small isometric virus particles containing double-stranded RNA have been independently reported in Europe and Japan from apparently healthy alfalfa (Medicago sativa), beet (Beta vulgaris), and white clover (Trifolium repens). They have been called cryptic viruses in Europe and temperate viruses in Japan. Serological comparison using immunoelectron microscopy, and polyacrylamide gel electrophoresis of the RNAs indicate that alfalfa cryptic and temperate viruses are the same, beet cryptic virus is probably a mixture of two different viruses, one of which is similar to or the same as beet temperate virus, and white clover temperate virus is a mixture of at least three different viruses, two of them indistinguishable from white clover cryptic viruses 1 and 2, respectively, and the third very likely the same as white clover cryptic virus 3.

Electrophoresis, Polyacrylamide Gel↗
PubMed1985

Morphology and nucleic acid of rice gall dwarf virus.

In negative stain preparations, the particles of rice gall dwarf virus (RGDV) had the form of angular icosahedra, approximately 65 nm in diameter, and resembled Phytoreovirus particles. The genomic double-stranded RNA of RGDV was fractionated by PAGE into 12 segments similar to those of the RNAs of the phytoreoviruses, wound tumor virus and rice dwarf virus, but of different mobilities. The RNAs were distinct from those of other plant reoviruses. RGDV therefore is confirmed as a new Phytoreovirus.

Molecular Weight↗
PubMed1981

Enhancement of the immunogenicity of the maize rough dwarf virus outer shell with the cross-linking reagent dithiobis(succinimidyl)propionate.

Injection into rabbits of native or glutaraldehyde (GA)-treated intact particles of the fijivirus maize rough dwarf virus (MRDV) failed to yield antibodies to the outer shell of the virus. After fixation of the particles with the cross-linking reagent dithiobis(succinimidyl)propionate (DSP), antisera were obtained reacting with the outer shell of MRDV (both DSP-fixed and unfixed) to a titre of 1/1024 in immunoelectron microscopic decoration tests, but giving no reaction with another fijivirus, oat sterile dwarf virus, whose subviral particles are known to be unrelated to those of MRDV. Further fixation of the DSP-fixed particles with GA did not appear to enhance immunogenicity. Stabilization with DSP could have further application with viruses the instability of which renders them poor immunogens.

Animals↗
PubMed1980

Electrophoretic fractionation of the double-stranded RNA genome of rice ragged stunt virus.

The genomic nucleic acid of rice ragged stunt virus (RRSV) was purified directly from enations of RRSV-infected plants. Its resistance to DNase and solubility in 2 M LiCl, plus its sharp thermal denaturation transition, with am melting temperature of about 83 degrees C in 0.15 m NaCl - 0.015 M Na citrate buffer, pH 7.0, showed the nucleic acid to be double-stranded RNA. On PAGE in 5% gels the RNA separated into 8 segments which occurred in equimolar amounts and had a total molecular weight of 11.63 x 10(6). RRSV resembles the fijiviruses, but its genome differs somewhat from those of other group members.

Molecular Weight↗
PubMedDate not supplied

[Socio-occupational adjustment in patients undergoing dialysis in the Piedmont region. Preliminary data derived from our questionnaire].

All working-aged patients in Piedmont receiving dialysis treatment were asked to fill in a questionnaire which aimed to highlight socio-working adjustment by assessing not only the optimal nature of dialytic treatment but also its repercussions in psycho-affective, socio-economic and cultural terms. The results of the study are reported together with preliminary comments.

Adolescent↗