Search PubMed⌕ Search

Biomedical subjects

G Blake

Publications and source records attributed to G Blake.

52 records · Page 3Linked to original sources
PubMed1988

Intracerebral hemorrhage in a primate model: effect on regional cerebral blood flow.

The dynamic changes in regional cerebral blood flow (rCBF), induced by a developing intracerebral hematoma, were studied in eight anesthetized monkeys. Hematomas were generated by allowing femoral arterial blood to enter the caudate nucleus via a stereotactically implanted needle. Intracranial pressure peaked at 51 +/- 8 mmHg at 3 minutes after the ictus, and remained high throughout the 3-hour procedure. Cerebral blood flow was significantly reduced in all brain regions for 1 hour after the ictus. The lowest rCBF values were recorded in the immediate clot penumbra and were below threshold levels for ischemic neuronal damage for 90 minutes after the hemorrhage.

Animals↗
PubMed1986

Tricyclic antidepressant agranulocytosis.

Severe neutropenia is an unusual complication of antidepressant therapy. We report a case of agranulocytosis due to clomipramine, and discuss principles of management.

Agranulocytosis↗
PubMed1985

Brain specific gravity and CT scan density measurements after human head injury.

White matter specific gravity was measured using the microgravimetric method in 20 comatose patients with diffuse head injury who were undergoing intracranial pressure (ICP) monitoring, and in 19 patients with focal injuries who were undergoing evacuation of contusions or intracerebral hematomas. Computerized tomography (CT) density readings were obtained for each site of white matter sampling by locating the sampling site on the preoperative CT scan. A significant correlation was found between the specific gravity values and the CT density numbers (r = 0.775; p less than 0.001). Patients with focal injuries demonstrated reduced perifocal specific gravity, suggesting brain edema. The mean specific gravity in patients with diffuse injury was within the normal range. In 10 of 12 patients in whom the specific gravity was above the normal range, the CT density was also above the normal range. These data suggest that cerebral vascular engorgement is the cause of the high specific gravity. Six (60%) of this small subgroup of 10 patients also demonstrated a high ICP.

Adult↗
PubMed1981

The effect of cervical/vaginal secretions on measurements of lecithin/sphingomyelin ratio and optical density at 650 nm.

Free-flowing amniotic fluid collected vaginally can be used in a reliable way for determination of fetal pulmonary maturity. Lavaging the vaginal/cervical area with sterile saline and examining the lavage fluid for lecithin/sphingomyelin (L/S) spots showed no detectable spots in the supernatants (one exception) and barely detectable L/S spots in the sediment. Vaginal-cervical saline-wash fluids did not affect fluid L/S ratios. Lavaging the vaginal-cervical area with abdominal amniotic fluid did not affect the L/S ratio of the original amniotic fluid.

Amniotic Fluid↗
PubMed1981

The measurement of gastric emptying: a comparison of fixed scintillation detection and the double-sampling technique.

The gastric emptying of a fluid meal was measured simultaneously by fixed scintillation detection and the double-sampling technique in 14 normal subjects and 15 patients who had a vagotomy and drainage procedure. In the normal subjects good correlation was obtained between the two methods but in the operated group there was poor correlation owing to difficulty in measuring the initial rapid emptying phase by fixed scintillation detection. Modification of the fixed scintillation method allows it to be used accurately in postoperative patients. The radioactivity of the meal is measured in vitro before ingestion and this reading is converted, using a constant derived from normal subjects, to a baseline value to which the subsequent in vivo readings are related. This modified technique permits the investigation of gastric emptying of fluid meals in patients with postvagotomy syndromes without the discomfort of a nasogastric tube. The equipment required is inexpensive.

Chromium Radioisotopes↗
PubMed1978

The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.

A cloned library of large, random embryonic human DNA fragments was constructed and screened for beta-globin sequences using the cloned human beta-globin cDNA plasmid pJW102 (Wilson et al., 1978) as a hybridization probe. Two independent clones were obtained and then characterized by restriction endonuclease cleavage analysis, hybridization experiments and partial DNA sequencing. Each of the clones carries both the adult delta- and beta-globin genes. The two genes are separated by approximately 5.4 kilobases (kb) of DNA and their orientation with respect to the direction of transcription is 5'-delta--beta-3'. Both the delta- and beta-globin genes contain a large noncoding intervening sequence (950 and 900 bp, respectively) located between the codons for amino acids 104 (arginine) and 105 (leucine). Although the location of the large intervening sequence within the coding regions of the two genes is identical, the two noncoding sequences bear little sequence homology. A second, smaller intervening sequence similar to that found in other mammalian beta-globin genes was detected near the 5' end of the human beta-globin gene. The two independently isolated beta-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the delta-globin gene of one of the clones. This suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the Pst I restriction enzyme recognition sequence.

DNA Restriction Enzymes↗
PubMedDate not supplied

A clinical profile of back pain and disability in patients with spinal osteoporosis.

This study investigated the pain and disability experienced by 85 consecutive postmenopausal Caucasian women, average age 64 years (range 50-82), with spinal osteoporosis, referred to a specialist bone clinic. Symptoms were related to the numbers and severity of vertebral deformities, and degree of kyphosis. Persistent back pain was experienced by 54 (63%) in the lumbar spine and 53 (62%) in the thoracic spine. The severity of thoracic but not lumbar back pain was significantly related by Kendall rank correlation to degree of kyphosis (R = 0.255, p < 0.01), numbers of collapsed vertebrae (R = 0.279, p < 0.01) and a summed score of the severity of collapse for all vertebrae (R = 0.253, p < 0.01). The severity and duration of thoracic back pain was also significantly related to numbers of collapsed vertebrae from T4-T8 alone. Sleep was disturbed in 60%, difficulty obtaining suitable clothes was found in 42% and difficulties with functional activities in 47%, although these were severe in only 10%. Significant associations were also found between scores of osteoporosis severity and sleep disturbance, difficulties with clothes and many functional activities. We conclude that in patients with spinal osteoporosis coming to medical attention, assessment and management of pain, disability, sleep pattern and difficulties with obtaining clothes are required for optimal care.

Activities of Daily Living↗