Biomedical subjects
Publications and source records attributed to G Berghaus.
A technique for PGM1 subtyping of blood stains by isoelectric focusing on 100 microns polyarylamide gel over a distance of 4 cm is demonstrated. There are savings of about 60% regarding material, time, and blood stain volume as compared to the 10-cm distance.
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A half-automatic technique for extracting characteristics from handprints is presented. Characteristics of the mainline system are defined from the co-ordinate aggregates. The practicability of the method in the anthropologic-heredobiological expert opinion is discussed.
A comparison of phospholipid content of sera from heart blood and sinus blood from the brain with the rate of haemolysis in the blood samples, with age, sex, degree of arteriosclerosis and cause of death, showed a relationship with the cause of death in spite of wide dispersion of the single values. The remaining factors of the 145 unselected human cadavers, which were not putrefied, seemed to have no influence. Small amounts of haemolysis caused a decrease of phospholipid concentration whereas an extensive haemolysis clearly led to an increase. The results of 46 deaths by hanging confirmed the fundamental studies of Berg (1950, 1952), who demonstrated that an increased secretion of phospholipids during the strangulation process is to be interpreted as a vital phenomenon. Compression of cervical vessels resulted in statistically significant differences between phospholipid concentrations in serum of heart and sinus blood.
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On the basis of 185 deaths caused by falls from height we examined the probability to differentiate between suicide and accident. Variables about the person, about the course and the background of the action as well as about the injury-pattern were taken into consideration. Contingency table analysis, chilsqare tests and probportional reduction in error measure were used first to determine the significant differences in criminal classification: sex, job, domicile, hour of the day, locality and height of the fall and the injuries established by postmortem examination. To test the discriminative power of the identified characteristics discriminant function analysis was used. Utilizing only 11 characteristics 88,6 percent of the deaths could be classified correctly.
Veins of the limbs of 193 sudden deaths are examined concerning the frequency, extent and localisation of sclerosis. The macroscopic process of preparation has been satisfactory. Correlations to several factors, especially age and diseases of heart and arteries, are presented and discussed.
Veins of the limbs of 193 sudden deaths are examined under aspects of frequency, extent and localisation of sclerosis. Macroscopic process of preparation has approved. Correlations to several factors, especially age and affections of heart and arteries, are represented and discussed.
Samples from the right lobe of the liver and from fat tissue of the abdominal wall were removed shortly after death from 116 persons. Fatty-acid pattern of both tissues (lauric, myristic, palmitic, palmitoleic, stearic, oleic, linoleic acids) was measured and compared with the degree of fatty infiltration of the liver. The data were also correlated with chronic excess alcohol consumption in the pre-terminal phase. It was found that with increasing fatty inflitration there was a rise in the relative proportion of palmitoleic acid in liver and subcutaneous fat tissues, with a fall in the relative proportion of stearic acid in the latter. Comparison of results in alcoholics (19 subjects) and non-alcoholics (91) showed the expected higher fatty infiltration of the liver in the former (x equal 13.4% and 5.6%, respectively). Both in liver and subcutaneous fat tissues the relative proportion of palmitoleic acid was significantly higher in alcoholics. But while in these the proportion of palmitoleic acid in the liver was higher than in subcutaneous tissue, the relationship was the converse among the non-alcoholics.
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A 3 month old child was treated in hospital for "toxikosis and prolonged gastroenteritis". The child required a special diet. The parents picked up the child in hospital and brought it home against the device of the doctors. 26 hours later the child had died of severe exsikkosis (loss of 17% of body weight within one day). Using immunological methods (Ouchterlony test) we were able to demonstrate the child was fed with contraindicated food although the doctors had pointed out that the child needs a special diet. The parents were sentenced at court in two instances for manslaughter.
Species- and sex-determination on hair roots were simultaneously performed using extracted DNA and a human Y chromosomespecific probe. (pH Y 2.1) After Southern hybridization, Hae III-digested DNA fragments were detected by non-radioactive digoxigenin detection system. DNA was extracted from one to five freshly plucked hair roots. The specific 2.12 kb fragment was successfully detected in male DNA samples from a single hair root. A positive identification of female DNA was more difficult. The hair root DNA was revealed to be stable at room temperature for at least 2 weeks (examination time) and produced the same specific band pattern as the DNA of fresh hair roots. In the blind tests with DNA samples from randomly plucked one to four hair roots, the rate of successful sex-determination was 95.8% on male samples (23 out of 24 samples) and 25% on female samples (4 out of 16 samples).
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