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Biomedical subjects

G Berg

Publications and source records attributed to G Berg.

At least 235 records · Page 13Linked to original sources
PubMed1980

Conformational change of the thyroglobulin molecule induced by oxidation in vitro.

Previous electron-microscopical studies from this laboratory have shown that the thyroglobulin molecule can occur in two different conformations, one ovoid and the other cylindrical. Ovoid molecules are characteristic of well-iodinated thyroglobulin whereas cylindrical molecules are found after low-iodine diet or blocking of iodination. The present study was performed in order to elucidate the possible relation between the molecule conformation and the peroxidase-catalyzed reactions that occur in the thyroid in connection with hormone synthesis. Cylindrical thyroglobulin molecules (from PTU-exposed thyroids) were incubated in different media and the proportion of cylindrical and ovoid molecules after incubation was estimated in electron micrographs. It was found that incubation with glucose-glucose oxidase caused an extensive conversion of cylindrical molecules into ovoid molecules. Peroxidase and/or iodide were not necessary for this change of conformation. It is suggested that this in vitro molecule transformation was the result of an oxidation reaction.

Animals↗
PubMed1980

Destruction by anaerobic mesophilic and thermophilic digestion of viruses and indicator bacteria indigenous to domestic sludges.

In raw sludges and in mesophilically and thermophilically digested anaerobic sludges, large variations in numbers of viruses occurred over narrow ranges of numbers of fecal coliforms, total coliforms, and fecal streptococci, demonstrating that the bacteria were poor quantitative reflectors of the numbers of the viruses detected. Mesophilic and thermophilic digestion of anaerobic sludges destroyed all three indicator bacteria more rapidly than such digestion destroyed the viruses. The relative rates for the destruction of viruses, fecal coliforms, and fecal streptococci in the digested sludges were consistent over the 17-month study. Fecal coliforms were 7 to 8 times more sensitive than the viruses to mesophilic digestion and 9 to 10 times more sensitive to thermophilic digestion. Total coliforms were even more sensitive. The rates at which fecal streptococci were destroyed by mesophilic and thermophilic digestion of anaerobic sludges approached those at which the viruses were destroyed by those processes; this suggested that the rates at which fecal streptococci in sludges are destroyed by those processes may serve as useful indicators for the rates at which viruses in sludges are destroyed by those processes.

Journal Article↗
PubMed1980

Method for recovering viruses from river water solids.

Small numbers of virions (poliovirus 1) that had been adsorbed to river water solids were eluted by mixing the solids for 30 min with a 10% solution of beef extract that contained sufficient Na2HPO4 to bring the molarity of the salt to 0.05 and sufficient citric acid to bring the pH to 7. The virions were recovered by inoculating the beef extract onto cell cultures. With this method, 39 to 63% of the poliovirions that had been adsorbed onto the river water solids were recovered.

Adsorption↗
PubMed1980

Effects of non-selective and selective beta-adrenergic agonists on spontaneous contractions and cyclic AMP levels in myometrial strips from pregnant women.

Isoprenaline and terbutaline relaxed myometrial strips from pregnant women and increased their content of cAMP. The highest measured cAMP concentration, about twice the control level, was determined after a 3-min incubation. Subsequently, the cAMP level declined and approached the basal level after about 60 min. The spontaneous contractions of the myometrial strips were abolished for about 20 min, but reappeared gradually after that time, although the beta-agonists were present in the incubation medium all the time. The decrease of the beta-adrenoceptor responses was not due to an inactivation of the drug tested, or dependent on any diffusible inhibitor substance, since the incubation medium containing the beta-agonist from the 60-min incubation elicited a normal response when added to a fresh myometrial preparation. We suggest that in vitro incubation with high concentrations of beta-agonists induces a desensitization of the beta-receptor of human myometrium.

Adrenergic beta-Agonists↗
PubMed1979

[Serum concentration of lactate in diabetic patients treated with biguanides in relationship to renal function (author's transl)].

Lactate, pyruvate, creatinine, urea, blood sugar and the serum concentration of phenformin or buformin were analyzed in the venous blood of a total of 90 diabetics who received biguanides regularly for at least one month. The creatinine clearance as well as the lactate-pyruvate quotient were computed. Age, body weight and size as well as duration of medication were recorded. Twenty-three diabetics who did not receive biguanides were used for control. Almost all patients revealed a mostly minor hyperlactatemia which was also observed in the control group. There was no significant difference between the two groups. There was no relationship between renal function, the serum lactate level and the biguanide concentration.

Aged↗
PubMed1979

Treatment of diabetic ketoacidosis with a computer-controlled bedside monitoring and infusion system.

A metabolic monitoring system is described that allows the simultaneous and automatic in vivo analysis of the metabolic parameters glucose, pH, and pCO2 as well as the computer-controlled automation of the possible necessary infusion therapy. This bedside system has proved to be useful in treating acidotically and non-acidotically decompensated diabetes mellitus. Our experience with this system is as follows: 1. quick correction of the acidosis without danger of alkalosis, 2. shorter period of treatment, 3. considerably reduced insulin requirement, 4. no danger of hypoglycemia, and 5. minimum net blood withdrawal (1.2 ml/h). The efficiency of this system is demonstrated by examples.

Adult↗
PubMed1979

Computer-controlled bedside monitoring and infusion system.

A mult-channel system for continuous bedside monitoring of glucose, pH pCO2 and automatic computer-controlled infusion of insulin, glucose, potassium, and bicarbonate solutions is described. Regulatory algorithms are presented for correction of metabolic acidosis and hyperglycemia with and without acidosis.

Acidosis↗
PubMed1979

Role of adrenals and estrogen in regression of mammary tumors during postpartum lactation in the rat.

The effects of bilateral adrenalectomy or estradiol benzoate treatment were observed on growth of 7,12-dimethylbenz(a)-anthracene-induced mammary tumors during postpartum lactation. In the control and estradiol benzoate-treated postpartum lactating rats, the mammary tumors decreased approximately 40% in size by Day 5 postpartum and continued to regress to 50% of their average original diameter by Day 25 postpartum. Adrenalectomy on Day 3 postpartum prevented mammary tumor regression and resulted in renewed mammary tumor growth. By Day 10 postpartum, average mammary tumor size in the adrenalectomized rats reached prepartum diameter and continued to increase in size until Day 25. Although serum prolactin concentrations were significantly higher in the lactating rats with mammary tumors than in the nonlactating rats with mammary tumors, there were no significant differences in serum corticosterone values. Adrenalectomy resulted in a significant increase in serum prolactin levels and in a marked fall in serum corticosterone levels. It is concluded that in rats adrenocortical activity is primarily responsible for reduced mammary tumor growth during postpartum lactation.

Adrenal Glands↗
PubMed1979

[Behavior of the serum fatty acid patterns during administration of an MCT-containing fat emulsion].

After a continuous intravenous 3-hour infusion of 500 ml fat emulsion containing MCT, composed of 25 g soybean oil and 25 g MCT oils, there was an infusion-induced increase of all fatty acids administered. The medium-chain fatty acids (C8-C12) revealed ideal steady-state conditions and were quickly eliminated after the infusion. The reaction of linoleic acid (C18:2) was not as favorable. Linoleic acid increased steadily during the infusion, and its elimination rate from the serum was delayed.

Caproates↗
PubMed1978

[Procetofen, a new lipid- and urine-acid-reducing substance].

23 patients with different forms of hyperlipidemias received procetofen (3 X 100 mg/day) over a period of fourteen days. The cholesterol concentration in blood showed a significant reduction from 322 +/- 30 mg/dl to 227 +/- 37 mg/dl, triglycerides dropped from 221 +/- 84 mg/dl to 129 +/- 34 mg/dl and uric acid diminished from 6,1 +/- 1,4 mg/dl to 4,6 +/- 0,8 mg/dl.

Cholesterol↗
PubMed1978

[Behavior of blood amino acids and fatty acids during complete parenteral hyperalimentation with carbohydrates, amino acids and fats].

Over a period of 12 hours a total amount of 190 g fructose, 190 g glucose, 145 g xylitol, 96 g fat and 99 g amino acids were infused. The utilization of carbohydrate was not diminished despite the high fat load. In addition, the antiketogenic action of carbohydrate was still provable. The nitrogen balance was positive as an indicator of good utilization of amino acids. Therefore, simultaneous administration of a carbohydrate mixture and a fat emulsion as energy sources during application of amino acids is recommended.

Acid-Base Equilibrium↗