Search PubMed⌕ Search

Biomedical subjects

G Benoit

Publications and source records attributed to G Benoit.

At least 181 records · Page 10Linked to original sources
PubMed1989

[Lymphocele and kidney transplantation].

The authors report fourteen cases of lymphocele in a series of one thousand consecutive renal transplantations (1.4%). The prevention of these lymphoceles depends on rigorous lymphostasis. The diagnosis is based on ultrasonography. The treatment indicated in cases of voluminous lymphoceles consisted in marsupialization and omentoplasty.

Adult↗
PubMed1989

[Principles of organ preservation].

Hypothermia remains the basic principle of all organ preservation. This review shows some main cellular mechanisms involved with normo and hypothermia, particularly during ischemia. Different pathways and target sites concerned in preservation are explained. Commonly used preservation techniques and cold storage solutions are also discussed. Cryopreservation especially vitrification seems to be promising perhaps in the near future; it allows a state of almost a cellular metabolic arrest and consequently a theoretically unlimited preservation time.

Body Temperature↗
PubMed1989

[Value of the scanner in acute pyelonephritis].

Twenty patients with clinical acute pyelonephritis were investigated by routine CT scan. This examination was positive in 19 cases, demonstrating either a hypodense triangular image reflecting a disorder of blood supply or a mass syndrome. The CT scan can therefore confirm the diagnosis of acute pyelonephritis. It also defines the severity of the lesions and helps to guide treatment.

Acute Disease↗
PubMed1989

[Resection of the prostate after 75 years].

The authors report a series of 100 endoscopic resections performed in patients over the age of 75 years. In patients with no risk factors, there was no mortality and the morbidity was 5%. In patients with one risk factor, mortality was 5% and morbidity was 10%.

Aged↗
PubMed1989

[Analysis of lymphocyte subpopulations of the bladder following endovesical immunotherapy using BCG].

The authors studied, by means of immunohistochemical analyses, the lymphocyte subpopulations in bladder biopsies from 20 patients treated for a urothelial bladder tumour (Tis TA, T1) by intravesical BCG (group A). The biopsies were taken from optically healthy zones and were then frozen. The lymphocyte populations in the urothelium and chorion were studied by means of monoclonal antibodies T3, T4, T8, TQ1, Leu-7, OKM-1, HLA-DR, TAC, Pan-B. Four patients without bladder cancer constituted the control group (group B) and 10 patients with bladder cancer not treated by intravesical BCG constituted group C. In group A, the T4 + TQ1 lymphocytes predominated in the urothelium and in the chorion. An inversion of the T4/T8 ratio was observed in the urothelium of groups B and C (p less than 0.01) and in the chorion of group B (p less than 0.01). There was no significant difference in the T4/T8 ratio in the chorion in groups A and C. In group A, 17 patients expressed the presence of receptors for interleukin 2 (TAC + HLA-DR) on their T lymphocytes. In these cases, the lymphocytic infiltration was associated with the presence of eosinophils (p less than 0.05) and the total absence of NK cells was observed. No B lymphocytes were detected, apart from a few rare lymphoid follicles.

Administration, Intravesical↗
PubMed1988

Enhancement of adriamycin antitumor activity by its binding with an intracellular sustained-release form, polymethacrylate nanospheres, in U-937 cells.

We investigated the antitumor activity of Adriamycin on a monocytic-like cancer cell line U-937 after its binding on polymethacrylate nanospheres (diameter, 270-350 nm). Compared to free Adramycin (F-ADR), nanosphere-bound Adriamycin (B-ADR) exhibits a 3-fold enhancement of cytotoxicity, as determined by cell growth inhibition and DNA synthesis, after continuous exposure to 0.02 and 0.04 microgram/ml. The 90% growth inhibition concentration was 0.051 microgram/ml for F-ADR and was 0.018 microgram/ml for B-ADR (P less than 0.001). Furthermore, the nanosphere densities per cell play an important role since for the same drug concentration the higher the density increases, the better the activity is. Indeed, after 4 days of incubation in a medium containing 160 nanospheres at 0.5 fg/cell, the cell counts were 62.8 +/- 12.8% (SD) of the initial inoculum and they were only 16.1 +/- 0.1% after incubation in a medium containing 800 nanospheres at 0.1 fg/cell (P less than 0.001). A comparable enhancement of activity regarding the nanosphere densities was observed after a 24-h exposure to 0.02 and 0.05 microgram/ml. Short-term uptake studies showed that B-ADR accumulation was higher with B-ADR than with F-ADR. In addition, the efflux kinetics was modified. For cells exposed to F-ADR for 4 h, the efflux half-life was 23.7 +/- 7.7 h and the area to infinity under the efflux curve was 8.6 +/- 2.8 micrograms/mg protein x h-1. For cells exposed to B-ADR, the efflux half-life increased to 85.9 +/- 19.2 h and the area to infinity under the efflux curve to 29.6 +/- 6.6 micrograms/mg protein x h-1 (P less than 0.001). Electron transmission microscopy and previous findings have revealed that B-ADR was well internalized into cells. Our data support the hypothesis that B-ADR acts as an intracellular drug release complex after endocytosis. The findings regarding the number of nanospheres per cell and dose-effect relationships are consistent with mechanisms of drug actions extending to membrane domains.

Cell Compartmentation↗
PubMed1988

Mycotic aneurysm and renal transplantation.

A case of mycotic aneurysm secondary to suppuration of a renal transplant is reported. This aneurysm was responsible for ischemia of the leg and was treated successfully by ligation and venous bypass grafting.

Adult↗
PubMed1988

Anatomical study of the infra-montanal urethra in man.

Five samples infra-montanal prostatic urethra fixed in formalin and stored in liquid nitrogen at -30C were examined histologically. The results of this study demonstrated that muscle fibres of the striated sphincter extend as far as the level of the verumontanum. The proportion of rapid and slow striated fibres was identical. These striated fibres were mixed with smooth muscle fibres of the urethra. The density of innervation of this muscular tunic was found to increase closer to the striated sphincter; it is composed of equal numbers of adrenergic and cholinergic fibres.

Adrenergic Fibers↗