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Biomedical subjects

G Bendixen

Publications and source records attributed to G Bendixen.

At least 37 records · Page 2Linked to original sources
PubMed1984

Antibodies against nuclear components in schistosomiasis. Results compared to values in patients with rheumatoid arthritis, systemic lupus erythematosus, and osteoarthrosis.

Occurrence of autoantibodies against nuclear material was compared in groups of patients with rheumatoid arthritis (RA n = 22), systemic lupus erythematosus (SLE n = 24), osteoarthrosis (OA n = 25), and chronic schistosomiasis mansoni (CSM n = 28). Anti-ds DNA antibody was detected by an ammonium sulphate precipitation radioimmunoassay antibodies against extractable nuclear antigen (ENA) were detected and differentiated in RNAse-resistant and RNAse-sensitive components (Sm and RNP antigens) with an ELISA technique. IgG organ-non-specific and granulocyte-specific antinuclear antibodies (ANA) were detected by immunofluorescence technique with quantitative titration of positive reactions and determination of complement-fixing properties. The results in groups of patients with SLE, RA and OA were of confirmative nature and supported that the different methods detect different systems of autoantibodies and nuclear autoantigens. In CSM it was demonstrated that 23 of 28 cases had positive reactions to the RNAse-resistant part of ENA (the Sm-antigen), a significant difference from the three other groups of patients (P less than 0.001). The antibody was in all cases of IgM class, in seven cases also of IgA class. Antibodies against nuclear material in CSM are probably a consequence of heavy disturbance of the immune system in this chronic infection with great permanent antigen load. It is a matter of discussion, whether production of these antibodies is induced by nuclear material from the host or from the parasite.

Adolescent↗
PubMed1984

Lack of evidence for human lymphokines with thrombocyte aggregating activity. An in vitro study.

The ability of human lymphocytes to produce soluble mediator(s) with thrombocyte aggregating activity (TAA) was investigated in an in vitro technique. Isolated human mononuclear cells were stimulated with phytohaemagglutinin or purified protein derivative of tuberculin. The supernatants were investigated for lymphokine activity in a leucocyte migration inhibitory factor assay. Supernatants were then tested for their ability to aggregate human thrombocytes, and in contrast to the results of previous studies, we were not able to demonstrate any TAA. Most likely, lymphokines with TAA are not involved in the thrombotic processes seen in human cell-mediated immune inflammatory reactions.

Adenosine Diphosphate↗
PubMed1983

Lymphocyte function and chromosome aberrations in patients with early mycosis fungoides and parapsoriasis en plaques.

Thirteen patients with stage I or II mycosis fungoides (MF) and 10 patients with large-plaque parapsoriasis en plaques (PEP) were examined for immunologic and cytogenetic disturbances. Total lymphocyte counts and immunoglobulin concentrations in the blood were normal. In vitro lymphocyte responses to polyclonal activators and various antigens in standard concentrations were normal. However, titration of phytohemagglutinin and concanavalin A (ConA) disclosed significantly lowered responses to suboptimal concentrations in the patient group, most pronounced in patients with MF II. ConA-induced leukocyte migration inhibitory factor (LIF) production, tested in an indirect leukocyte migration inhibitory assay, was low in the patient group. Furthermore spontaneous LIF production in vitro and small amounts of serum LIF were demonstrated in a few patients. The chromosomal banding pattern, sister chromatid exchange, and break frequency were within normal limits except for 3 translocations in the MF group. It is concluded that even in early-stage MF a pathologic function of blood lymphocytes can be demonstrated, when sensitive methods are applied. The findings might be important for monitoring disease activity and effect of treatment.

Adult↗
PubMed1982

Antibodies to SS-B in chronic inflammatory connective tissue diseases. Relationship with HLA-Dw2 and HLA-Dw3 antigens in primary Sjögren's syndrome.

SS-B antigen, purified from rabbit thymus, was used in an indirect enzyme immunoassay to demonstrate the presence of IgG-, IgA-, and IgM-type SS-B antibodies in sera from patients with well-defined and characterized chronic inflammatory connective tissue disease. High levels of antibodies to SS-B were found in patients with primary and secondary Sjögren's syndrome. Patients with Sjögren's syndrome secondary to systemic lupus erythematosus had significantly higher SS-B antibody values than patients with Sjögren's syndrome secondary to rheumatoid arthritis or patients with rheumatoid arthritis or systemic lupus erythematosus, alone. Two patients with rheumatoid arthritis without secondary Sjögren's syndrome also had a markedly elevated level of antibodies to SS-B. Antibodies of all immunoglobulin classes were found, although the highest values were either IgG- or IgM-type. In primary Sjögren's syndrome, antibody values to SS-B were higher in patients with HLA-Dw2 and/or HLA Dw3 than in those with other HLA-Dw types. We conclude that these antigens or specific immune-response genes close to the D region may be important for the development of antibodies to SS-B.

Antibodies↗
PubMed1982

Functional characteristics of synovial fluid and blood mononuclear cells in rheumatoid arthritis and traumatic synovitis.

The activity of blood mononuclear cells (BMC) and synovial fluid mononuclear cells (SMC) from patients with rheumatoid arthritis (RA) and traumatic synovitis (TS) was assessed by means of [14C]thymidine incorporation and production of leukocyte migration inhibitory factor (LIF). When compared with normal controls, spontaneous LIF production by BMC was found in 5 of 9 TS patients, whereas spontaneous LIF production by rheumatoid arthritis BMC and by SMC from both patient groups was infrequently seen. ConA-induced LIF production by BMC and SMC from both patient groups did not differ significantly from that of normal controls. Thymidine incorporation by unstimulated SMC and BMC was low in both patient groups. After stimulation with polyclonal activators, SMC showed significantly reduced proliferation in comparison with BMC, but the responses to microbial antigens were equal to or higher than those of BMC. The proliferative responses of stimulated SMC from TS patients were higher than the responses displayed by stimulated SMC from RA patients.

Adult↗
PubMed1980

Inflammatory, non-antibody lymphocytic mediators (lymphokines).

This paper is a survey of some of the best characterized non-antibody lymphocytic mediators (lymphokines). Special emphasis is laid on the description of the molecular biology of human inflammatory and antigen-nonspecific lymphokines, and where clinical relevant their methods of assay are summarized. The putative role of lymphokines in vivo is briefly discussed.

B-Lymphocytes↗
PubMed1979

Systemic lupus erythematosus--a zoonosis?

The medical history was obtained and a physical examination carried out--in addition to an immunological investigation of the blood and skin--on 11 dogs in the ownership of patients with systemic lupus erythematosus (SLE). The results were compared with those obtained from a control group and it was found that they do not support the hypothesis that SLE is a zoonosis.

Animals↗
PubMed1978

Human leucocyte response to migration inhibitory activity from lymphocytes. Modification by aprotinin, Tranexamic acid and phenylmethyl sulfonylfluoride.

Human lymphokines can elicit several effects associated with inflammation, e.g. leucocyte migration inhibition and fibrinolysis. These effects can be assessed in vitro by the leucocyte migration agarose technique (LMAT) and the leucocyte migration fibrinolysis technique (LMFT). The present study shows that preincubation of normal leucocytes with aprotinin, tranexamic acid and phenyl-methyl-sulfonylfluoride (PMSF) reduces or abolishes their migration inhibition response to leucocyte migration inhibition factor. The compounds exert this effect at non-toxic concentrations, which do not otherwise interfere with migration or fibrinolysis, and are non-toxic as estimated by PHA stimulation of lymphocytes. The LMFT is more sensitive to the modifying effect than the LMAT. The effect of aprotinin and tranexamic acid is reversible, the effect of PMSF is irreversible.

Aprotinin↗
PubMed1978

Humoral and cellular immunity in sarcoidosis.

The Kveim reaction was studied in vivo in 50 patients with sarcoidosis. Commonwealth Serum Laboratories Kveim material and a new Danish Kveim material gave 14 and 8 positive reactions respectively, as well as 6 and 8 equivocal reactions. Forty-six of the patients were also tested in vitro for cell mediated immunity to the Danish Kveim material, using both the leucocyte migration agarose technique and the capillary technique. No significant migration inhibition or stimulation were found. A tuberculin skin test was performed in 49 of the patients, and in 45 a dinitrochlorobenzene sensitivity titer was determined. Both tests revealed a depression of the cell mediated immunity. The serum levels of immunoglobulins IgG, IgA, IgM, IgD, and IgE were determined. The serum of each patient was also examined to determine if organ-non-specific and granulocyte-specific antinuclear factors of IgG class, antibodies against native DNA, rheumatoid factor, mitochondrial antibodies, antibodies against thyroid cytoplasm, and parietal cell antibodies were present. IgG levels were above normal in 28 patients; IgE was above normal in 10 patients, 4 of whom were atopics or had an atopic disposition. Organ-non-specific antinuclear factors were present in 17 patients.

Adult↗
PubMed1978

Anti-DNA antibody in serum measured by radioimmunoassay (Farr technique). Description of method and recommended procedure.

Anti-DNA antibody determination in serum is increasingly used because it supports the diagnosis of systemic lupus erythematosus (SLE) with high selectivity. The present work evaluates several of the technical variables of the Farr radioimmunoassay for anti-DNA antibody determination and describes a recommendable procedure, emphasizes the most important sources of error and gives the range of normal blood donors and a group of hospital patients without SLE.

Adult↗