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Biomedical subjects

G Bauer

Publications and source records attributed to G Bauer.

612 records · Page 34Linked to original sources
PubMedDate not supplied

Transformation of rat fibroblasts by TGF-beta and EGF induces sensitivity for intercellular induction of apoptosis.

During intercellular induction of apoptosis, TGF-beta-treated nontransformed fibroblasts induce apoptosis specifically in transformed fibroblasts. To test whether sensitivity to intercellular induction of apoptosis is causally related to the expression of the transformed phenotype, NRK 536 cells were reversibly transformed by TGF-beta / EGF- treatment and were tested for their sensitivity to intercellular induction of apoptosis. Cells that expressed the transformed phenotype after application of both cytokines exhibited sensitivity for intercellular induction of apoptosis, whereas cells treated with only one of the cytokines did not show the transformed phenotype and remained insensitive. Sensitivity to intercellular induction seems to be determined by extracellular generation of superoxide anions, as sensitive cells generated extracellular superoxide anions and intercellular induction of apoptosis in these cells was inhibited by SOD. These data demonstrated that the cytokine-determined transformed phenotype of fibroblasts controls their sensitivity for intercellular induction of apoptosis through induction of superoxide anion generation.

Animals↗
PubMedDate not supplied

Promyelocytic HL-60 cells induce apoptosis selectively in transformed cells: involvement of myeloperoxidase, nitric oxide and target cell-derived superoxide anions.

Promyelocytic HL-60 cells induced apoptosis selectively in transformed fibroblasts, but not in nontransformed parental cells or in revertants. Apoptosis induction was independent of direct cell-to-cell contact. It depended on the number of effector and target cells, pointing to the role of factors derived from both cell populations. Inhibitor studies allowed the establishment of the following model for intercellular signalling during apoptosis induction: target cell-derived superoxide anions spontaneously dismutate and form hydrogen peroxide. HL-60 effector cells release MPO which converts hydrogen peroxide into hypochlorous acid. Hypochlorous acid then may interact with target-cell-derived superoxide anions to yield apoptosis-inducing hydroxyl radicals. In addition, effector-cell-derived NO reacts with target-cell-derived superoxide anions and forms apoptosis-inducing peroxynitrite. These data explain the selective apoptosis induction in transformed cells by effector cells of the granulocyte lineage.

Anions↗
PubMedDate not supplied

[Carbon monoxide poisoning caused by a hot plate].

The autopsy of a woman aged 85 years who was found dead sitting in her kitchen bent over the kitchen-table revealed a fatal carbon monoxide poisoning. Subsequent inquiries excluded the chimney flue and above all the gas-fired water heater from being the CO-generator. Operating experiments upon the gas stove including the ultimately used pot and the Hot Plate likewise found showed a CO-content of 100 ppm in the room-air after half an hour, there upon an over-proportional ascent of the CO-concentration. This CO-production was brought about by the considerable deformation of the Hot Plate, which has obviously been used for years. The lack of an adequate warning in the directions for use of such products is remarked critically.

Aged↗
PubMedDate not supplied

[Fiber-rich snacks with reference to their effect on the digestive activity and blood lipids of the elderly].

32 subjects of a home for the elderly and nursing home were fed special test meals beside the usual home diet for 84 test days. The test meals, rich in dietary fibre, "Leinsamen" (linseed) and "Rohfasergemisch" (dietary fibre mix) are adequate for improving the subjects' digestion. The identified reductions are insignificant in regard to the effect on an increased serum triglyceride level. With the snack "Früchtemüesli" we achieved a highly significant, with "Leinsamen" a significant reduction of blood cholesterol level.

Aged↗
PubMedDate not supplied

[Clinical use of meniscus sonography].

We report on the sonography of the meniscus as a new non-invasive imaging technique for diagnosis of traumatic lesions of the menisci of the knee joint. Using an efficient ultrasound equipment with a 7.5 MHz sectorscanner, the sonographic imaging of all parts of the medial and the lateral meniscus is regularly successful. During 10 months more than 200 patients with symptoms of a traumatic meniscal lesion got a sonographic examination in our hospital. The results show that in 86% there is an identity with the operative findings. By sonographic imaging there can be seen longitudinal and transversal ruptures, 'fishmouth' - and 'bucket - handle' lesions and complete detachment of the meniscus. Decisive for the diagnosis is a hyperechogenic zone running through the homogeneous gray meniscus. With ultrasound, it is regularly possible to get information about the locality and the extension, but not about the kind of lesion.

Adolescent↗
PubMedDate not supplied

[Acute necrotic hemorrhagic pancreatitis. Major complications (author's transl)].

Forty-four cases of acute necrotic haemorrhagic pancreatitis are studied. Fourteen cases were treated medically by peritoneal dialysis, 20 were treated surgically of which 16 had been medically treated by peritoneal dialysis. Fifteen died or 34%. Forty-one patients, 93.1% presented 8 major complications on admission and 2 complications were observed during the course of medical treatment (pulmonary shock and high digestive haemorrhage). The post surgical complications are excluded from this study. We report in order of frequency; effusion of the large peritoneal cavity (37 cases : 84%), hypocalcaemia less than or equal to 8 mg% (21 cases : 47.7%), renal insufficiency defined by a creatinaemia greater than or equal 2 mg% (17 cases : 38.6%), state of shock (13 cases : 29.5%), severe neurological disorders (11 cases : 25%), peritoneal haemorrhage (3 cases : 4.5%), disseminated intravascular coagulation (1 case : 2.2%), acute rabdomyolysis (1 case : 2.2%). Certain cases are particularly derogatory : pulmonary shock : 2 cases -- 2 deaths (100%); hypocalcaemia less than or equal to 7 mg/ : 6 cases -- 5 deaths (83.3%); acute tubular necrosis : 8 cases -- 6 deaths (75%); hypocalcaemia less than or equal 8 mg% : 21 cases -- 12 deaths (57.1%); high digestive haemorrhage : 3 cases -- 1 death (33.3%); amber known brown peritoneal effusion : 27 cases -- 12 deaths (44.4%); shock : 13 cases -- 5 deaths (38.5%). When in the same patients, less than 3 complications were present, the mortality rate was 20.8%. If more than 3 signs were observed the mortality rate rose to 53.3%. Except for pulmonary shock, six major complications were needed to give 100% mortality rate.

Acute Disease↗
PubMedDate not supplied

TGF-beta isoforms and fibroblast growth factor exhibit analogous indirect antioncogenic activity through triggering of intercellular induction of apoptosis.

TGF-beta-1 has recently been shown to trigger nontransformed effector cells to induce apoptosis specifically in transformed cells. This intercellular induction of apoptosis has been discussed as a potential control step in oncogenesis. Here we show that triggering of intercellular induction of apoptosis is not a non-specific growth factor effect, but is restricted to the TGF-beta and FGF family of growth factors. Within the TGF-beta family, all isoforms triggered the intercellular induction of apoptosis with the same efficiency. This finding illustrates that these effects observed have been conserved throughout evolution, which thus points to their potential biological significance. The parallel action of TGF-beta and FGF in the intercellular induction of apoptosis correlates with the role of both families of factors in the establishment and maintenance of the transformed state. Autocrine loops of either growth factor seem to be recognized by the surrounding nontransformed cells and to evoke an apoptosis- inducing effect which thus prevents the survival and outgrowth of potential tumor cells.

Animals↗
PubMedDate not supplied

Fracture of the cuboid in children: case report and review of the literature.

Although fractures of the cuboid are rare, they can be very disabling. The diagnosis is often missed, and overlooked cuboid fractures can lead to severe alterations in foot mechanics and function. We report the case of a displaced compression fracture of the cuboid in an 8-year-old girl after a fall from a height, which we treated by open reduction, bone grafting, and internal fixation. Follow-up examination 2 years after the injury revealed a very good result. Cuboid fractures should be considered in the differential diagnosis of limping toddlers. Children seen with pain, swelling, and inability to bear weight on the lateral aspect of the foot require proper evaluation and treatment.

Accidental Falls↗