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Biomedical subjects

G Bauer

Publications and source records attributed to G Bauer.

At least 343 records · Page 19Linked to original sources
PubMed1993

[Impaired gait after base fractures of the 5th metatarsal bone].

Between 1982 and 1990, twenty fractures of the base of the fifth metatarsal were treated operatively and followed up for a mean of 4.5 years (range: 1-9 years). All patients were evaluated clinically, roentgenographically and with kinetic gait analysis. A scoring system was used to record and evaluate the clinical and radiological data. Nine patients had an excellent result and nine a good result; one patient had a fair and one a poor result. Examination by kinetic gait analysis showed a gait asymmetry in eleven of the twenty patients, with decreased load on the formerly injured areas of the involved foot and increased load on the corresponding areas of the contralateral foot. However, only three patients had a clinically visible gait disorder. We believe that the gait disorder described is an automatically adopted movement pattern. Its cause lies in the initial pain after the trauma and during the postoperative care and sometimes continues even after the cessation of pain. Kinetic gait analysis allows quantification of asymmetry of gait and clinically non-visible load disorder. Therefore, pain and established gait asymmetry are clinically relevant in such patients because they can be treated specifically.

Adolescent↗
PubMed1993

[Cerebellar atrophy and phenytoin poisoning. An MR study].

Phenytoin has been considered a possible cause of cerebellar degeneration, especially after clinical intoxication. Magnetic resonance provides the diagnosis of anatomical structures in the posterior fossa without the limitation of beam hardening artefacts. The aim of this study was to evaluate the relationship of phenytoin medication and cerebellar atrophy in 11 patients with increased serum levels (21.4 micrograms/ml-95.6 micrograms/ml). Five patients had normal cerebellar structures, although three of them had a history of clinical intoxication and all had at least one episode of increased serum level of DPH. The remaining six patients had moderate severe cerebellar atrophy (n = 4) and atrophy of the vermis cerebelli (n = 5). Two of them had never experienced clinical intoxication. There was no correlation between the degree of atrophy and severity of clinical symptoms and evaluation of serum DPH levels (up to four times normal values). There was also no correlation between cerebellar atrophy, duration of epilepsy and frequency of seizures. We conclude that phenytoin overdosage does not necessarily result in cerebellar atrophy and it is unlikely that phenytoin medication was the only cause of cerebellar atrophy in the remaining patients.

Adult↗
PubMed1993

[Management of unstable forearm shaft fractures in children].

Complete dislocation fractures of the forearm are rare in children. Operative treatment is indicated if it is an open fracture and if neurovascular injury is present. There is no accepted standard treatment--operative or nonoperative--for fractures that cannot be accurately reduced and stabilized. In the last 10 years we have operated on 37 fractures of the forearm in children. In 22 cases the indication for operative treatment was that the fractures could either not be reduced or could not be stabilized. Our results show that most fractures were not recognized as unstable (17 out of 22 cases), and therefore several reduction maneuvers (2 to 4 times) were performed before definitive stabilization was obtained via an operation. It is therefore mandatory that unstable fractures of both bones or of one bone be operated on whereas semistable fractures (one bone completely fractured) should be reduced under general anesthesia and operative standby. For the surgical treatment of unstable forearm fractures we recommend intramedullary fixation by dynamic nailing or plate osteosynthesis in younger patients (5-10 years) and open reduction and plate osteosynthesis in older patients and in open fractures or fractures with primary neurovascular impairment.

Adolescent↗
PubMed1992

Transformation of rodent fibroblasts by herpes simplex virus: presence of morphological transforming region 1 (MTR 1) is not required for the maintenance of the transformed state.

Studies on the mechanisms of transformation of mammalian cells by herpes simplex virus (HSV) in vitro have been prevented so far by the extremely low transformation frequencies obtained in monolayer culture. Here we present a transformation system that relies on the direct seeding in soft agar of infected single cells, thus avoiding negative interactions between normal and transformed cells. We took advantage of HSV-I temperature-sensitive mutants at the UL9 locus, which codes for a DNA-binding protein necessary for viral DNA replication. At the non-permissive temperature, viral DNA synthesis and late gene expression are prevented. Viral gene expression is restricted to immediate early and early genes. Induction of transformation was highly efficient in our one-step transformation system. It depended on intact viral particles and viral DNA. Immediate early and/or early viral gene expression was sufficient to induce transformation. Colonies were stably transformed and did not show any rescue of viable virus after temperature downshift and co-cultivation with susceptible cells. Transformed cells maintained the transformed state in the absence of viral DNA. Our data therefore support the "hit-and-run" hypothesis for the transforming effect of HSV.

Animals↗
PubMed1992

Reactivity to TGF-beta is one step towards transformation in a murine fibroblast cell line.

High doses of TGF-beta induce reactivity to TGF-beta in a distinct number of C3H10T1/2 fibroblasts suspended in soft agar. Reactive cells can be isolated and stably maintain this property. In the absence of exogenously added TGF-beta, these cells do not form colonies, but show distinct changes in their morphology and cytoskeleton. In contrast to normal C3H10T1/2 cells, they are able to form colonies when low doses of TGF-beta are added. TGF-beta-reactive cells are more easily transformed by UV light than normal C3H10T1/2 cells, and so maintain their transformed phenotype in the absence of added TGF-beta. Reactivity for TGF-beta, therefore, represents one step towards transformation.

Animals↗
PubMed1992

Microbial metabolism of quinoline and related compounds. XV. Quinoline-4-carboxylic acid oxidoreductase from Agrobacterium spec.1B: a molybdenum-containing enzyme.

The quinoline-4-carboxylic acid oxidoreductase from Agrobacterium spec.1B was purified 84-fold to apparent homogeneity with 15% recovery, using ammonium sulphate precipitation, heat precipitation, hydrophobic interaction, anion exchange- and gel chromatography. The molecular mass of the native enzyme was estimated to be 320 kDa by gel filtration. SDS-polyacrylamide gel electrophoresis of the enzyme revealed three protein bands corresponding to 85, 35 and 21 kDa. Per molecule the enzyme contains 8 atoms of iron, 8 atoms of acid-labile sulphur, 2 atoms of molybdenum, 2 molecules of FAD and as molybdenum cofactor, molybdopterin cytosine dinucleotide. Besides quinoline-4-carboxylic acid the enzyme also catalysed the conversion of quinoline, 4-chloroquinoline and 4-methylquinoline to the corresponding 2-oxo-1,2-dihydroderivatives. Cyanide, methanol, 4-chloromercuribenzoate and acriflavin were effective inhibitors.

Chromatography, Gel↗