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Biomedical subjects

G Bastert

Publications and source records attributed to G Bastert.

At least 235 records · Page 13Linked to original sources
PubMed1988

Gene amplification and expression of the neu (c-erbB-2) sequence in human mammary carcinoma.

Alterations in the structure and expression of the neu oncogene were investigated in 15 human mammary tumors. In 7 of the samples (46%), amplification by the factor up to 14 was detected, some samples displaying an additional EcoRI restriction fragment at 3.4 kb. In tumor tissue with amplified neu sequences from which intact RNA was available, enhanced expression was noted. Correlation of the molecular data to clinical parameters indicated a correlation of the neu oncogene amplification to tumor grading and thus poor prognosis.

Breast Neoplasms↗
PubMed1988

[Transvaginal echography. An application in the diagnosis of cervical incompetence].

It has already been demonstrated that abdominal ultrasound is an adequate method for measuring the cervix in pregnancy. The use of the vaginal route for such measurement is new. We have been using for the vaginal approach an ultrasound transducer of 5 mHZ with an angle of sweep of 240 degrees. The study was carried out on 23 pregnant women at the 28th week of pregnancy who had been admitted because of threatened premature labour with possible cervical incompetence; and on 20 cases of normal pregnancy also at the 28th week, which is the time when we do glucose estimations normally in our antenatal clinics. The result of our work shows that this ultrasonic method through the vagina gives us much more accurate measurements of the cervix than digital vaginal examination does. A mean difference of 12.3 mm in the length of the cervix was found in normal pregnancies (46.3 mm) and in cases where there was a question of cervical incompetence (34 mm). Furthermore the phenomena of shortening, dilatation of the cervix and a funnel-shape of the internal os are ultrasound signs of value in diagnosing incompetent cervix. Further advantages of the transvaginal approach are that there is no need for the bladder to be filled previously, and that the organs are nearer which improves the ultrasound picture. All the same, it is to be emphasized that transvaginal ultrasound can only be a factor to be added to clinical examination in the diagnosis of the incompetent cervix.

Cervix Uteri↗
PubMed1986

Maternal serum alpha-fetoprotein levels in a triplet pregnancy with 2 papyraceous fetuses.

Serum Alpha-Fetoprotein (AFP) was found to rise to exceedingly high levels in a case of triplet pregnancy after two fetuses died in the 21st week of gestation. The surviving infant was born in the 36th week accompanied by the two fetus papyracei. By the time the process of mummification of the two dead fetuses appeared to be complete on ultrasound, the maternal AFP level had returned to the normal range for singleton pregnancies. HCG, hPL, and estriol and the coagulation profile remained within the normal range throughout the pregnancy.

Adult↗
PubMed1985

Human mammary carcinomas in nude rats--a new approach for investigating oxygen transport and substrate utilization in tumor tissues.

A new model is presented for the study of oxygen supply and substrate utilization in human tumor tissue. In this approach human tumor material thrives in immune-deficient nude rats. The host chosen allows the continuous evaluation of all relevant parameters. From the data obtained so far it is concluded that this model is a valid tool in investigation of the metabolic status of human tumors.

Animals↗
PubMed1984

[Pregnancy and birth of monozygous female twins following in vitro fertilization and embryo transfer].

The rate of identical twins after in vitro fertilisation and embryo replacement is higher than after physiological conception. The present paper reports on the first delivery of twins (identical twins) after in vitro fertilisation and embryo replacement. The in vitro fertilisation and embryo replacement of one 4-cell stage resulted in a twin pregnancy. The difficult pregnancy, early delivery and intense perinatal care of the children deserves special consideration. In addition the paper gives a survey on the present number of deliveries after in vitro fertilisation and embryo replacement given at the 3rd World Congress on In Vitro Fertilisation and Embryo Transfer at Helsinki in May 1984.

Adult↗
PubMed1983

In vitro cultivation of xenotransplanted human breast-cancer cells.

Xenotransplanted human breast-cancer tissue growing in the second to the ninth passage in nu/nu mice and normal tissue from nu/nu mice was cultivated in vitro. Thirty-two of fifty-two (62%) cultures grew successfully and have been analyzed. The cells were observed using phase-contrast microscopy and May-Grünwald-Giemsa staining. The following cell types could be distinguished: normal fibroblast-like cells (FC), normal epithelial-like cells (EP I), abnormal epithelial-like cells (EP II), and degenerated or dying cells (DC). After staining, the nuclear area of all cell types was measured. The results were: FC = 280 micrometers 2, EP I = 320 micrometers 2, and EP II = 800 micrometers 2. They were compared with the nuclear area of human breast-cancer cells obtained by aspiration biopsy.

Animals↗
PubMed1983

The use of human fetal pancreas for transplantation: experimental and clinical results.

Human fetal pancreata (15-22 weeks of gestation) were precultivated in vitro for 7 to 25 days and transplanted subcutaneously to thymusaplastic nude mice and an insulin-dependent diabetic patient. In mice, precultivated xenotransplanted pancreata showed typical transplant growth in the recipients. Histology revealed typical islets formation. Whereas without precultivation human fetal pancreas older than 16 weeks showed no transplant growth, after precultivation human fetal pancreas even up to 22 weeks of gestation developed. Transplantation of a precultivated human fetal pancreas to a diabetic patient was without effect, e.g. the required insulin dose remained unchanged after transplantation and no C-peptide was detectable.

Adult↗
PubMed1980

Transplantation of human fetal pancreas: experience in thymusaplastic mice and rats and in a diabetic patient.

The aim of the present study was to investigate if thymusaplastic nude mice and rats are favorable as recipients for transplantation of human fetal pancreas. Twenty human fetal pancreases were transplanted subcutaneously to 20 nude mice, and six human fetal pancreases were transplanted to six rats. The xenografts showed histotypical development of islets of Langerhans. Insulin, glucagon, somatostatin, and pancreatic polypeptide immunoreactivities were also seen in very early stages of the transplant development within the monolayered ducts. With the described "epigastric pouch technique" in rats, we co-ld demonstrate a new in vivo method for selective stimulations and simultaneous blood sampling from tissue-isolated xenografts. Transplantation of human fetal pancreas to the brachioradial muscle of an insulin-dependent patient in combination with a kidney transplant revealed that rejection crises of the kidney led to necrosis of the pancreas transplant, whereas rejection of the kidney was overcome by steroid pulse therapy.

Animals↗