Enzyme immunoassay of albumin as an aid to the diagnosis of cystic fibrosis in the newborn human [proceedings].
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Biomedical subjects
Publications and source records attributed to G B Wisdom.
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The bases of the different enzyme-immunoassay techniques are described and their applications to the identification and quantification of antigens, haptens, and antibodies in biological fluids are discussed. Substances for which enzyme-immunoassays have been developed are listed. The enzymes used as labels, the methods of linking them to proteins, and the methods for separating free and bound labeled material are described. The reliability and practicality of the enzyme-immunoassay methods are reviewed.
The indirect immunofluorescent technique has been shown to be a more sensitive method than either immunoelectrophoresis or gel filtration for testing the efficacy of the gel filtration method in fractionating human immunoglobulins. It has been confirmed that IgM fractions of some sera from patients with multiple sclerosis, contain antibody which reacts with measles virus-infected tissue culture cells.
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