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Biomedical subjects

G B KOELLE

Publications and source records attributed to G B KOELLE.

At least 19 recordsLinked to original sources
PubMed1960

Acetylcholinesterase regeneration in peripheral nerve after irreversible inactivation.

The return of acetylcholinesterase activity was studied in several cholinergic structures in the cat after irreversible inactivation by diisopropylfluoro-phosphate. It was found that enzymic activity returned uniformly along the hypoglossal and cervical sympathetic nerve trunks. No evidence for somatoaxonal convection of enzyme was obtained.

Acetylcholinesterase↗
PubMed1959

The cytological localization of intracellular neuronal acetylcholinesterase.

Sections of cat ciliary ganglia were stained for acetylcholinesterase activity by several modifications of the acetylthiocholine method in order to achieve optimal accuracy of cytological localization of the enzyme. These were compared by ordinary light and phase contrast microscopy with similar sections stained by standard techniques for Nissl substance, the Golgi apparatus, and the neurofibrillae, and by intravital methylene blue. The pattern of cytoplasmic distribution of acetylcholinesterase corresponded most closely with that of the Nissl substance. Following total inactivation of the ganglionic acetylcholinesterase by intravenously administered di-isopropyl fluorophosphate, the reappearance of the enzyme in vivo occurred at the same cytoplasmic sites prior to its reappearance at the cell membrane or preganglionic axonal terminations. These observations, and reports cited from the literature, provide support for the hypothesis that acetylcholinesterase is synthesized within the endoplasmic reticulum, then transported via its canaliculi to the surface of the cell and its processes, where its functional sites are oriented externally to the lipoidal membrane.

Acetylcholinesterase↗